Dissimilar effects of curcumin on human granulosa cells: Beyond its anti-oxidative role

Moreira-Pinto, Beatriz; Costa, Liliana M.; Fonseca, Bruno M.; Rebelo, Irene

doi:10.1016/j.reprotox.2020.04.069

Cited by 10 publications

(5 citation statements)

References 34 publications

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“…In order to evaluate morphological changes at the cellular level resulting from RES exposure, Giemsa staining was performed [21]. Moreover, to evaluate nuclear changes Höechst staining was performed [29].…”

Section: Morphological Analysismentioning

confidence: 99%

“…After 24 h, for the mitochondrial mem-brane potential (∆Ψ m ) study, RES was added (5-20 µM) and the cells incubated for another 24 or 48 h. The mitochondrial membrane-depolarizing agent CCCP (10 µM) was used as a positive control [30]. Cells were incubated with CCCP for 15 min before adding DiOC 6 probe for 30 min at 37 • C [21]. Fluorescence was then measured in a Microplate Fluorimeter (BioTek Instruments, Winooski, VT, USA) (excitation: 488 nm; emission: 525 nm).…”

Section: Morphological Analysismentioning

confidence: 99%

“…OS plays an important role both in cases of pathology-associated infertility and in cases of idiopathic infertility [19]. Since it is known that a sufficient intake of antioxidants can decrease the risk of ovulatory infertility, women with fertility complications tend to self-medicate with antioxidant supplements [20,21].…”

Section: Introductionmentioning

confidence: 99%

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Low Doses of Resveratrol Protect Human Granulosa Cells from Induced-Oxidative Stress

et al. 2021

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Resveratrol is a phytoalexin present in plant-derived foods, including grape’s skin, cocoa, and peanuts. Evidence suggests that it has beneficial effects on human health because of its antioxidant properties. However, there is limited knowledge about the part played by resveratrol in ovarian function. In this paper, the influence of resveratrol on granulosa cells (GC) was evaluated. In addition to being the main estradiol producers, GC are in direct contact with the oocyte, playing a fundamental role in its growth and development. The cell line COV434 and human granulosa cells (hGC), obtained from women undergoing assisted reproductive technology (ART), were used. GC were treated with resveratrol (0.001–20 μM) at different times (24–72 h). Low concentrations of this compound suggest a protective role, as they tend to reduce ROS/RNS formation after inducement of stress. On the contrary, high concentrations of resveratrol affect GC viability and steroidogenic function. As it may act as a direct modulator of GC oxidative balance, this work may help to clarify the impact of resveratrol on GC and the usefulness of this antioxidant as adjunct to infertility treatments.

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Section: Morphological Analysismentioning

confidence: 99%

Section: Morphological Analysismentioning

confidence: 99%

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Low Doses of Resveratrol Protect Human Granulosa Cells from Induced-Oxidative Stress

et al. 2021

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“…An antioxidant compound that is receiving particular attention is curcumin (CMN), which has substantial anti-inflammatory, immunomodulatory, antitumoral, antipsoriatic, and wound-healing properties in addition to its antioxidant activity [28][29][30][31][32][33][34][35]. CMN is a delicious yellow-orange-colored spice with a wide range of pharmacological and biological activities.…”

Section: Introductionmentioning

confidence: 99%

Protective effects of curcumin against methotrexate-induced testicular damage in rats by suppression of the p38-MAPK and nuclear factor-kappa B pathways

Kilinc

2021

Clin Exp Reprod Med

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Objective: Methotrexate (MTX), is a commonly used chemotherapeutic agent. This study aimed to investigate the possibility that curcumin (CMN) protects against MTX-induced testicular damage by affecting the phospho (p)-p38 mitogen-activated protein kinase (MAPK) and nuclear factor-kappa B (NF-kB) signaling pathways. Methods: Male Wistar albino rats were subdivided into three groups. The control group was given an intragastric (ig) administration of dimethyl sulfoxide (DMSO) daily for 14 days, the MTX group was given a single intraperitoneal (ip) dose of MTX (20 mg/kg) on the 11th day, and the MTX+CMN group was given ig CMN (100 mg/kg/day, dissolved in DMSO) for 14 days and a single ip dose of MTX (20 mg/kg) on the 11th day. Results: The animal weights, the seminiferous tubule diameter, and germinal epithelium height significantly decreased in the MTX group compared to the control group. The testes weight and the ratio of the testes to body weight did not change, whereas the number of seminiferous tubules and the interstitial space width increased significantly in the MTX group. The number of phospho-p38 (p-p38) MAPK immunopositive cells and the immunoreactivity of NF-kB also increased in the MTX group compared to the control group. Conclusion: CMN prevented the MTX-induced decreases in the body weight, seminiferous tubule diameter, and the germinal epithelium height, while significantly reducing the number of histologically damaged seminiferous tubules and the interstitial space width changes due to MTX. CMN also reduced the number of p-p38 MAPK immunopositive cells and the NF-kB immunoreactivity.

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“…Vashist et al (2018), on the other hand, found that adding 50 µM curcumin to buffalo granulosa cells reduced cell viability. Curcumin @50 µM dosages (24 h and 48 h) and 10 µM doses (72 h) were found to be cytotoxic in the human granulosa cells by Moreira-Pinto et al (2019). Shi et al (2006), on the other hand, found that this action was beneficial in human ovarian cancer cells, as curcumin in the regular diet reduced the proliferation of human ovarian cancer cells.…”

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confidence: 99%

Influence of curcumin and carbazole on ovine ovarian preantral follicle and granulosa cell functions

Nandi

Gupta

et al. 2021

Indian J of Anim Sci

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The present study was undertaken to study the effect of plant bioactive compounds curcumin and carbazole on sheep ovarian functions. In the present study, both the bioactive compounds were tested at different levels (Control, T1-1 μM, T2-5 μM, T3-10 μM, T4- 25 μM, T5- 50μM, T6-100 μM) on preantral follicle (PF) growth rate, survival rate (6 days culture), granulosa cell (GC) number increment (2 days culture) and estradiol production (5 days GC culture spent media). Curcumin had shown a significantly higher PF survival rate (%), i.e. 74.3±1.5, 76.3±1.4 at 10 and 25 μM levels respectively. Similarly, higher PF growth rates (μm per day), i.e. 16.1±0.9 was observed at 50 μM levels. Similarly, curcumin was effective @ 50 μM level to increase the granulosa cell number as well as estradiol production with a mean granulosa cell number (×105) and estradiol production (pg) values of 1.55±0.04 and 85.3±3.3 respectively. Likewise, carbazole was effective at the level of 25 μM to increase the PF growth rate (μm per day), survival rate (%)with mean values of 74.3±1.3, 12.1±0.9. Similarly, carbazole was effective at 50 μM dose levels in the granulosa cell number increment (×105) with a mean value of 1.57±0.02. No significant change in estradiol production was observed in carbazole treated group.

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Dissimilar effects of curcumin on human granulosa cells: Beyond its anti-oxidative role

Cited by 10 publications

References 34 publications

Low Doses of Resveratrol Protect Human Granulosa Cells from Induced-Oxidative Stress

Low Doses of Resveratrol Protect Human Granulosa Cells from Induced-Oxidative Stress

Protective effects of curcumin against methotrexate-induced testicular damage in rats by suppression of the p38-MAPK and nuclear factor-kappa B pathways

Influence of curcumin and carbazole on ovine ovarian preantral follicle and granulosa cell functions

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