Dissemination of the rmtB gene carried on IncF and IncN plasmids among Enterobacteriaceae in a pig farm and its environment

Yao, Qiongfen; Zeng, Zhenling; Hou, Jianxia; Deng, Yuting; He, Liang-Ying; Tian, Wei; Zheng, Hongqing; Zhang-liu, Chen; Liu, Jianhua

doi:10.1093/jac/dkr328

Cited by 32 publications

(22 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Apart from the Bolivian region investigated in our work, consistent dissemination of CTX-M-65 has so far been limited to China and other Asiatic countries (i.e., Japan and Korea), where it is among the dominant CTX-M variants in enterobacteria of animal origin (36,(41)(42)(43)(44). The reasons accounting for the peculiar CTX-M-65 epidemiology, characterized by the distribution in two distant geographical areas, remain unknown.…”

Section: Discussionmentioning

confidence: 80%

Characterization of IncI1 Sequence Type 71 Epidemic Plasmid Lineage Responsible for the Recent Dissemination of CTX-M-65 Extended-Spectrum β-Lactamase in the Bolivian Chaco Region

Riccobono

Pilato

Maggio

et al. 2015

Antimicrob Agents Chemother

View full text Add to dashboard Cite

f During the last decade, a significant diffusion of CTX-M-type extended-spectrum ␤-lactamases (ESBLs) was observed in commensal Escherichia coli from healthy children in the Bolivian Chaco region, with initial dissemination of CTX-M-2, which was then replaced by CTX-M-15 and CTX-M-65. In this work, we demonstrate that the widespread dissemination of CTX-M-65 observed in this context was related to the polyclonal spreading of an IncI1 sequence type 71 (ST71) epidemic plasmid lineage. The structure of the epidemic plasmid population was characterized by complete sequencing of four representatives and PCR mapping of the remainder (n ‫؍‬ 16). Sequence analysis showed identical plasmid backbones (similar to that of the reference IncI1 plasmid, R64) and a multiresistance region (MRR), which underwent local microevolution. The MRR harbored genes responsible for resistance to ␤-lactams, aminoglycosides, florfenicol, and fosfomycin (with microevolution mainly consisting of deletion events of resistance modules). The bla CTX-M-65 module harbored by the IncI1 ST71 epidemic plasmid was apparently derived from IncN-type plasmids, likely via IS26-mediated mobilization. The plasmid could be transferred by conjugation to several different enterobacterial species (Escherichia coli, Cronobacter sakazakii, Enterobacter cloacae, Klebsiella oxytoca, Klebsiella pneumoniae, and Salmonella enterica) and was stably maintained without selective pressure in these species, with the exception of K. oxytoca and S. enterica. Fitness assays performed in E. coli recipients demonstrated that the presence of the epidemic plasmid was apparently not associated with a significant biological cost. C TX-M-type ␤-lactamases, first described in the late 1980s, have become the most common extended-spectrum ␤-lactamases (ESBLs) among Enterobacteriaceae worldwide (1, 2). Multiple sublineages (groups) of CTX-M-type ESBLs exist, and their dissemination has followed complex trajectories, depending on the spreading ability of the epidemic plasmids and bacterial clones with which bla CTX-M genes have become associated (1). Paradigmatic examples are the dissemination of bla CTX-M-15 , mediated by IncFII and IncX plasmids associated with the Escherichia coli sequence type 131 (ST131) clone (3, 4); the dissemination of bla CTX-M-14 in Spain and the United Kingdom, mediated by IncK plasmids (5-8); and the dissemination of bla CTX-M-65 in China, mediated by F33:A-:B-plasmids (9).In South America, members of the CTX-M-2 group emerged first and underwent wide dissemination during the 1990s (1, 10-12). Subsequently, members of the CTX-M-1 group (mostly CTX-M-15) and of the CTX-M-9 group have also disseminated in the area, outnumbering and replacing CTX-M-2 (13-18).A similar epidemiological evolution was also observed in the Bolivian Chaco region, where we have been monitoring the evolution of antibiotic resistance among commensal E. coli isolates during the past 2 decades (from 1992 to 2011) (19)(20)(21). In that area, CTX-M-type ESBLs were first detected in 2002 and ...

show abstract

Section: Discussionmentioning

confidence: 80%

Characterization of IncI1 Sequence Type 71 Epidemic Plasmid Lineage Responsible for the Recent Dissemination of CTX-M-65 Extended-Spectrum β-Lactamase in the Bolivian Chaco Region

Riccobono

Pilato

Maggio

et al. 2015

Antimicrob Agents Chemother

View full text Add to dashboard Cite

show abstract

“…Furthermore, there is potential for horizontal transmission of plasmid-borne genes contributing to their distribution and abundance in soil (11). A number of previous studies have unambiguously shown that soils receiving animal manures or biosolids are enriched in antibiotic resistance genes (13,(16)(17)(18)(19)(20). A few studies have characterized gene abundance over time following application under field conditions.…”

Section: Discussionmentioning

confidence: 99%

Safely Coupling Livestock and Crop Production Systems: How Rapidly Do Antibiotic Resistance Genes Dissipate in Soil following a Commercial Application of Swine or Dairy Manure?

Marti

Tien

Murray

et al. 2014

Appl Environ Microbiol

115

View full text Add to dashboard Cite

Animal manures recycled onto crop production land carry antibiotic-resistant bacteria. The present study evaluated the fate in soil of selected genes associated with antibiotic resistance or genetic mobility in field plots cropped to vegetables and managed according to normal farming practice. Referenced to unmanured soil, fertilization with swine or dairy manure increased the relative abundance of the gene targets sul1, erm(B), str(B), int1, and IncW repA. Following manure application in the spring of 2012, gene copy number decayed exponentially, reaching background levels by the fall of 2012. In contrast, gene copy number following manure application in the fall of 2012 or spring of 2013 increased significantly in the weeks following application and then declined. In both cases, the relative abundance of gene copy numbers had not returned to background levels by the fall of 2013. Overall, these results suggest that under conditions characteristic of agriculture in a humid continental climate, a 1-year period following a commercial application of raw manure is sufficient to ensure that an additional soil burden of antibiotic resistance genes approaches background. The relative abundance of several gene targets exceeded background during the growing season following a spring application or an application done the previous fall. Results from the present study reinforce the advisability of treating manure prior to use in crop production systems.

show abstract

“…Moreover, the majority of reports on dissemination of armA and rmtB to various Enterobacteriaceae species were focused on E. coli isolated from chickens and pigs (Yao et al, 2011; Yang Y. et al, 2015). Previous research has reported the occurrence of the ArmA methyltransferase in an ST11 clone of K. pneumoniae isolated from cats and dogs in Spain (Hidalgo et al, 2013a).…”

Section: Introductionmentioning

confidence: 99%

Clonal Spread of 16S rRNA Methyltransferase-Producing Klebsiella pneumoniae ST37 with High Prevalence of ESBLs from Companion Animals in China

et al. 2017

View full text Add to dashboard Cite

We screened 30 Klebsiella pneumoniae isolates from dogs and cats at a single animal hospital in Guangdong Province, China. Among them, 12 K. pneumoniae strains possessed high-level resistance to amikacin and gentamicin and these were screened for 16S rRNA methyltransferase (16S-RMTase) genes. And then the genes positive isolates were detected for ESBLs (extended spectrum β-lactamases) and analyzed by pulsed-field gel electrophoresis, multilocus sequence typing, PCR-based replicon typing and plasmid analysis. The genetic profiles of rmtB were also determined by PCR mapping. The twelve 16S-RMTase gene-positive isolates were rmtB (11/30) and armA (2/30) with one isolate carrying both genes. Extended spectrum β-lactamases genes were represented by blaCTX-M-55 (9/12), blaCTX-M-27 (2/12) and blaCTX-M-14 (1/12). The twelve 16S-RMTase containing strains were grouped into five clonal patterns and ST37 was the most prevalent sequence type. Ten rmtB-bearing plasmids conjugated successfully and all belonged to IncN and IncF (F33:A-:B-) incompatibility groups. Nine of the transconjugants carried a 97 kb plasmid and the other harbored both ∼60 and ∼200 kb plasmids. rmtB and blaCTX-M-55 were present on the same plasmid and indicated the co-transfer of these two genes, with the rmtB gene showing highly relevant relationships with IS26 and Tn3. Our findings suggested a high prevalence of 16S-RMTase genes in K. pneumonia ST37 from dogs and cats. Additional studies are needed to trace the evolutionary path of this type of resistance among the K. pneumonia isolates, and to determine whether they have been transferred to humans.

show abstract

Dissemination of the rmtB gene carried on IncF and IncN plasmids among Enterobacteriaceae in a pig farm and its environment

Cited by 32 publications

References 25 publications

Characterization of IncI1 Sequence Type 71 Epidemic Plasmid Lineage Responsible for the Recent Dissemination of CTX-M-65 Extended-Spectrum β-Lactamase in the Bolivian Chaco Region

Characterization of IncI1 Sequence Type 71 Epidemic Plasmid Lineage Responsible for the Recent Dissemination of CTX-M-65 Extended-Spectrum β-Lactamase in the Bolivian Chaco Region

Safely Coupling Livestock and Crop Production Systems: How Rapidly Do Antibiotic Resistance Genes Dissipate in Soil following a Commercial Application of Swine or Dairy Manure?

Clonal Spread of 16S rRNA Methyltransferase-Producing Klebsiella pneumoniae ST37 with High Prevalence of ESBLs from Companion Animals in China

Contact Info

Product

Resources

About