Background:-Conjunctivitis (red eye) is one of common eye disorders presented to Emergency Department. It is an infectious disease in most cases and can be transmitted within the emergency department between nurses and patients. Awareness and knowledge of any disease especially infectious type play important role in prevention of occurrence such this disease. Objectives:-To assesses awareness and knowledge of causes, signs and symptoms of red eyes (conjunctivitis) and its associated factors among emergency nurses. Subjects and methods: This cross-sectional study included all nurses representing different nationalities and both genders working at Emergency departments of major hospitals of Ministry of Health in Taif-city. The study was carried out at the largest two general hospitals in Taifcity, belonging to Ministry of health namely King Faisal and King Abdul-Aziz specialized hospitals. The study was carried out throughout the period October-November, 2016. Data were collected through a valid self-report questionnaire. Results:-One hundred and fifty-one nurses out of 201, responded by completing the questionnaire with a response rate of 75.1%.The age of them ranged between 22 and 50 years with a mean±SD of 32.1±5.0 years. Most of them (84.1%) were females. Almost two thirds of nurses were non-Saudis (70.9%). As regard etiology of conjunctivitis, themost known were microbial organisms such asbacteria/viruses (88.7%) while the least known were ocular trauma (41.7%). Regarding signs and symptoms, the most correctly recognized was eye turn reddish (92.7%) while the least recognized was blurred vision (49.7%). Collectively, 37.7%, 27.3% and 23.8% of nurses had insufficient knowledge regarding conjunctivitis causes, signs/symptoms and overall, respectively Conclusion:-Knowledge of red eye (conjunctivitis) among emergency nurses is insufficient particularly regarding its etiology. Therefore, inorder to control infection in emergency room, their education in this regard is highly recommended.