Dissemination of IncF plasmids carrying beta-lactamase genes in  Gram-negative bacteria from Nigerian hospitals

Ogbolu, David Olusoga; Daini, OA; Ogunledun, A; Alli, O. A. Terry; Webber, Mark A.

doi:10.3855/jidc.2613

Cited by 24 publications

(19 citation statements)

References 34 publications

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“…Recent studies reported CTX-M-type ESBLs in E. coli clinical isolates from hospitals, the community and healthy foodproducing animals in Nigeria often associated with fluoroquinolone resistance [3,4,13]. In E. coli isolates from outpatients from Southeastern Nigeria, the association of bla CTX-M-1 ESBL genes with bla TEM , bla OXA-1 and aac(6 0 )-Ib-cr genes were observed [14].…”

Section: Introductionmentioning

confidence: 92%

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A novel plasmid carrying blaCTX-M-15 identified in commensal Escherichia coli from healthy pregnant women in Ibadan, Nigeria

Fortini

Fashae

Villa

et al. 2015

Journal of Global Antimicrobial Resistance

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The aim of this study was to investigate the molecular characteristics of commensal Escherichia coli producing extended-spectrum β-lactamases and showing fluoroquinolone resistance circulating in a healthy population in Ibadan, Nigeria. In total, 101 faecal samples from healthy pregnant women on the day of admission to hospital were collected and plated on eosin-methylene blue agar supplemented with cefotaxime. Genotyping demonstrated the presence of the bla gene in all of the cefotaxime-resistant isolates (n=32), and there was circulation of prevalent clones. The aac(6')-Ib-cr, qnrS1, qepA1 and qnrB1 genes were identified in several strains. A novel plasmid supporting the spread of the bla, bla and qnrS1 genes was identified in these isolates by complete DNA sequencing.

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Section: Introductionmentioning

confidence: 92%

“…The increasing incidence of community-acquired infection due to ESBL-producing bacteria represents a great concern [1][2][3][4][5][6][7][8].…”

Section: Introductionmentioning

confidence: 99%

A novel plasmid carrying blaCTX-M-15 identified in commensal Escherichia coli from healthy pregnant women in Ibadan, Nigeria

Fortini

Fashae

Villa

et al. 2015

Journal of Global Antimicrobial Resistance

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“…IncF plasmids are narrow‐host‐range plasmids that rely on both host‐encoded and self‐encoded factors for replication . They are usually large in size (>100 kb), but with low copy number and often carry an additional replicon type to initiate replication . This a strategy used by narrow‐host‐range plasmids to obtain broad‐host‐range replication.…”

Section: Plasmid Biology and Incompatibility Groupsmentioning

confidence: 99%

Plasmid evolution in carbapenemase‐producing Enterobacteriaceae: a review

Kopotsa

Sekyere

Mbelle

2019

Annals of the New York Academy of Sciences

170

178

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Carbapenem‐resistant Enterobacteriaceae (CRE) have been listed by the WHO as high‐priority pathogens owing to their high association with mortalities and morbidities. Resistance to multiple β‐lactams complicates effective clinical management of CRE infections. Using plasmid typing methods, a wide distribution of plasmid replicon groups has been reported in CREs around the world, including IncF, N, X, A/C, L/M, R, P, H, I, and W. We performed a literature search for English research papers, published between 2013 and 2018, reporting on plasmid‐mediated carbapenem resistance. A rise in both carbapenemase types and associated plasmid replicon groups was seen, with China, Canada, and the United States recording a higher increase than other countries. blaKPC was the most prevalent, except in Angola and the Czech Republic, where OXA‐181 (n = 50, 88%) and OXA‐48–like (n = 24, 44%) carbapenemases were most prevalent, respectively; blaKPC‐2/3 accounted for 70% (n = 956) of all reported carbapenemases. IncF plasmids were found to be responsible for disseminating different antibiotic resistance genes worldwide, accounting for almost 40% (n = 254) of plasmid‐borne carbapenemases. blaCTX‐M, blaTEM, blaSHV, blaOXA‐1/9, qnr, and aac‐(6′)‐lb were mostly detected concurrently with carbapenemases. Most reported plasmids were conjugative but not present in multiple countries or species, suggesting limited interspecies and interboundary transmission of a common plasmid. A major limitation to effective characterization of plasmid evolution was the use of PCR‐based instead of whole‐plasmid sequencing–based plasmid typing.

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“…The extended-spectrum β-lactamases (ESBLs), especially those of the CTX-M type, are often associated with plasmid-mediated quinolone resistance (PMQR) and aminoglycoside resistance genes. Plasmids belonging to the IncF group have been the primary hosts for these traits (Boyd et al, 2004 ; Yao et al, 2011 ; Matsumura et al, 2013 ; Ogbolu et al, 2013 ).…”

Section: Introductionmentioning

confidence: 99%

IncF plasmid diversity in multi-drug resistant Escherichia coli strains from animals in China

Qin

Sun

et al. 2015

Front. Microbiol.

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The purpose of this study was to characterize a collection of 103 multidrug resistance IncF plasmids recovered from Escherichia coli of food producing and companion animals between 2003 and 2012. A total of 103 incF plasmids were characterized using an established PCR-based IncF replicon sequence typing (RST) system to identify FII, FIA, and FIB (FAB) groups. Plasmids were also analyzed using-restriction fragment length polymorphism (RFLP). Antibiotic Resistance determinants blaCTX-M, plasmid-mediated quinolone resistance (PMQR) genes and rmtB and plasmid addiction systems (PAS) were identified by PCR screening. A total of 20 different RSTs from 103 IncF plasmids were identified. The groups F2 and F33 with the RST formulae A-: B- were the most frequently encountered types (63.1%). The antibiotic resistance genes (ARGs) blaCTX-M, rmtB, and oqxB were carried by 82, 37, and 34 IncF plasmids, respectively. Most of these plasmids carried more than one resistance gene (59.2%, 61/103). The IncF plasmids also had a high frequency of addiction systems (mean 2.54) and two antisense RNA-regulated systems (hok–sok and srnBC) and a protein antitoxin-regulated system (pemKI) were the most prevalent. Not surprisingly, RFLP profiles among the IncF plasmids were diverse even though some shared identical IncF-RSTs. This is the first extensive study of IncF plasmid-positive E. coli isolates from animals in China. Our results demonstrate that IncF is the most prevalent plasmid family in E. coli plasmids and they commonly carry multiple resistance determinants that render them resistant to different antibiotic classes simultaneously. IncF plasmids also harbor addiction systems, promoting their stability and maintenance in the bacterial host, under changing environmental conditions.

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Dissemination of IncF plasmids carrying beta-lactamase genes in Gram-negative bacteria from Nigerian hospitals

Cited by 24 publications

References 34 publications

A novel plasmid carrying blaCTX-M-15 identified in commensal Escherichia coli from healthy pregnant women in Ibadan, Nigeria

A novel plasmid carrying blaCTX-M-15 identified in commensal Escherichia coli from healthy pregnant women in Ibadan, Nigeria

Plasmid evolution in carbapenemase‐producing Enterobacteriaceae: a review

IncF plasmid diversity in multi-drug resistant Escherichia coli strains from animals in China

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