Dissemination of carbapenemase-producing Enterobacterales through wastewater and gulls at a wastewater treatment plant in Sweden

Woksepp, Hanna; Karlsson, Klara; Börjesson, Stefan; Lindsjö, Oskar Karlsson; Söderlund, Robert; Bonnedahl, Jonas

doi:10.1016/j.scitotenv.2023.163997

Cited by 4 publications

(1 citation statement)

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“…The presence of clinically relevant multidrug-resistant (MDR) bacteria in the environment is a growing concern, with wildlife, particularly wild birds, being viewed as important sentinels for AMR surveillance 5 , 6 . Although wild birds can acquire antimicrobial-resistant bacteria/genes, likely from foraging in anthropogenically impacted areas including both landfills and WWTPs 7 – 9 , the role of wild birds in the dissemination of clinically relevant AMR needs further investigation 10 . Several studies have suggested that wild birds could be competent vectors of AMR and potentially disperse AMR through their movements 8 , 11 , 12 .…”

Section: Introductionmentioning

confidence: 99%

Detection and genomic characterization of Klebsiella pneumoniae and Escherichia coli harboring tet(X4) in black kites (Milvus migrans) in Pakistan

Mansoor,

Lu,

Woksepp

et al. 2024

Sci Rep

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The emergence of plasmid-mediated tigecycline resistance gene tet(X4) among clinically relevant bacteria has promoted significant concerns, as tigecycline is considered a last-resort drug against serious infections caused by multidrug-resistant bacteria. We herein focused on the isolation and molecular characterization of tet(X4)-positive Klebsiella pneumoniae (K. pneumoniae) and Escherichia coli (E. coli) in wild bird populations with anthropogenic interaction in Faisalabad, Pakistan. A total of 150 birds including black kites (Milvus migrans) and house crows (Corvus splendens) were screened for the presence of tigecycline resistance K. pneumoniae and E. coli. We found two K. pneumoniae and one E. coli isolate carrying tet(X4) originating from black kites. A combination of short- and long-read sequencing strategies showed that tet(X4) was located on a broad host range IncFII plasmid family in K. pneumoniae isolates whereas on an IncFII-IncFIB hybrid plasmid in E. coli. We also found an integrative and conjugative element ICEKp2 in K. pneumoniae isolate KP8336. We demonstrate the first description of tet(X4) gene in the WHO critical-priority pathogen K. pneumoniae among wild birds. The convergence of tet(X4) and virulence associated ICEKp2 in a wild bird with known anthropogenic contact should be further investigated to evaluate the potential epidemiological implications. The potential risk of global transmission of tet(X4)-positive K. pneumoniae and E. coli warrant comprehensive evaluation and emphasizes the need for effective mitigation strategies to reduce anthropogenic-driven dissemination of AMR in the environment.

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