Dissemination of antibiotic resistance genes, mobile genetic elements, and efflux genes in anthropogenically impacted riverine environments

Chaturvedi, Preeti; Chowdhary, Pankaj; Singh, Anuradha; Chaurasia, Deepshi; Pandey, Ashok; Chandra, Ram; Gupta, Pratima

doi:10.1016/j.chemosphere.2021.129693

Cited by 28 publications

(10 citation statements)

References 44 publications

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“…These results suggested that environmental factors such as tides or latitude did not the drive the occurrence of ARGs in these estuarine environments. Human activities is frequent in this area and people can either acquire ARGs through direct exposure when swimming there and also introduce new ARGs, so promoting their horizontal transfer of ARGs (Chaturvedi et al, 2021). In order to minimize the impact of human activities on the ARGs detected in RCWs, water samples were collected in winter to minimize the anthropogenic variation in the data.…”

Section: Args Patterns Was Strongly Affected By Bacterial Phylogeneti...mentioning

confidence: 99%

Occurrence and driving mechanism of antibiotic resistance genes in marine recreational water around Qinhuangdao, China

Han

Wen

et al. 2022

Front. Mar. Sci.

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Rapid urbanization and other human activities have exacerbated the global human health risks associated with antibiotic resistance genes (ARGs). Swimming in contaminated waters is one of important pathways by which humans can be exposed to ARGs. However, little is currently known about the overall levels of ARGs in recreational waters and the forces driving their occurrence. In this study, we analyzed the ARGs and bacterial communities in water samples taken from five recreational coastal waters (RCWs) and their adjacent estuaries in the Qinhuangdao area of Bohai Bay, China, using highthroughput quantitative PCR and 16S rRNA gene amplicon sequencing technique. The relative abundances of ARGs in RCWs varied greatly, and occurred at levels close to, or even higher than, those in the neighboring estuaries, ranging from 9.4×10 -2 to 2.5 copies/16S rRNA gene. A total of 159 different ARGs able to express resistance to almost all antibiotics were detected in all of the water samples. b-lactamase resistance genes (blaTEM gene) were the most abundance of all ARGs. Patterns of ARG occurrence were significantly correlated with those of bacterial communities (Mantel test, P < 0.05). A partial redundancy analysis showed that differences in bacterial communities accounted for 50.3% of the total ARG variations, which was much higher than the sum of the contribution of mobile genetic elements (18.1%) and the other selected environmental factors (8.3%). Principal coordinate analysis based on Bray-Curtis distance revealed the similarities between ARG profiles in the RCWs and in their adjacent estuaries. To our knowledge, this is the first study to report the ARG profiles in RCWs using high throughput qPCR technology. These results implied that it is necessary to develop proper regulations and monitoring strategies of RCWs for reducing risks of ARGs on human health. KEYWORDS antibiotic resistance genes (ARGs), mobile genetic elements (MGEs), bacterial community, recreational coastal water, human public health Frontiers in Marine Science frontiersin.org 01

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Section: Args Patterns Was Strongly Affected By Bacterial Phylogeneti...mentioning

confidence: 99%

Occurrence and driving mechanism of antibiotic resistance genes in marine recreational water around Qinhuangdao, China

Han

Wen

et al. 2022

Front. Mar. Sci.

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“…Gram-negative bacteria were isolated from wastewater samples on Eosin Methylene Blue agar (HiMedia, India). About 100 μL of wastewater sample was spread on selective agar plates in triplicate and incubated at 35–37°C for 24 h as per the methods described earlier ( Chaturvedi et al, 2021 ; Sivaraman et al, 2021 ). Morphologically distinct colonies based on appearance, color size, margins, texture, etc.…”

Section: Methodsmentioning

confidence: 99%

“…Escherichia coli and other Gram-negative bacteria resistant to β-lactams, flouroquinolones, tetracyclines and other classes of antibiotics have been reported from wastewaters, river waters and wastewater treatment plants from different states of India along with co-prevalence of ESBL genotypes and other Class A β-lactamases including bla CTX-M-15 , bla CTX-M-152 , bla CTX-M-205, bla SHV and bla TEM ( Bajaj et al, 2015 ; Hanna et al, 2020 ). Environmental monitoring revealed the presence of bacteria resistant to multiple antibiotics as well as bla CTX-M, bla TEM , bla SHV and other β-lactamase genotypes in water of river Ganga, Gomti, Yamuna and Hindon ( Chaturvedi et al, 2020 , 2021 ). The scientific data on the prevalence of antibiotic-resistant Gram-negative bacteria from irrigation-purpose wastewaters are either limited or lacking from Himachal Pradesh.…”

Section: Introductionmentioning

confidence: 99%

Prevalence of antibiotic-resistant Gram-negative bacteria having extended-spectrum β-lactamase phenotypes in polluted irrigation-purpose wastewaters from Indian agro-ecosystems

Avatsingh,

Sharma,

Kour

et al. 2023

Front. Microbiol.

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Antibiotic resistance in bacteria has emerged as a serious public health threat worldwide. Aquatic environments including irrigation-purpose wastewaters facilitate the emergence and transmission of antibiotic-resistant bacteria and antibiotic resistance genes leading to detrimental effects on human health and environment sustainability. Considering the paramount threat of ever-increasing antibiotic resistance to human health, there is an urgent need for continuous environmental monitoring of antibiotic-resistant bacteria and antibiotic resistance genes in wastewater being used for irrigation in Indian agro-ecosystems. In this study, the prevalence of antibiotic resistance in Gram-negative bacteria isolated from irrigation-purpose wastewater samples from Sirmaur and Solan districts of Himachal Pradesh was determined. Bacterial isolates of genera Escherichia, Enterobacter, Hafnia, Shigella, Citrobacter, and Klebsiella obtained from 11 different geographical locations were found to exhibit resistance against ampicillin, amoxyclav, cefotaxime, co-trimoxazole, tobramycin, cefpodoxime and ceftazidime. However, all the isolates were sensitive to aminoglycoside antibiotic gentamicin. Enterobacter spp. and Escherichia coli showed predominance among all the isolates. Multidrug-resistance phenotype was observed with isolate AUK-06 (Enterobacter sp.) which exhibited resistant to five antibiotics. Isolate AUK-02 and AUK-09, both E. coli strains showed resistant phenotypes to four antibiotics each. Phenotypic detection revealed that six isolates were positive for extended-spectrum β-lactamases which includes two isolates from Enterobacter spp. and E. coli each and one each from Shigella sp. and Citrobacter sp. Overall, the findings revealed the occurrence of antibiotic resistant and ESBL-positive bacterial isolates in wastewaters utilized for irrigation purpose in the study area and necessitate continuous monitoring and precautionary interventions. The outcomes of the study would be of significant clinical, epidemiological, and agro-environmental importance in designing effective wastewater management and environmental pollution control strategies.

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“…In different monitoring analyses regarding antibiotics in pristine waters, papers report in some cases the amount of antibiotic residues (in water and in sediments) and the resistance as gene quantification or as antibiotic susceptibility tests to some antibiotics also on bacterial isolates (Araújo Nogueira et al, in press;Chaturvedi et al, 2021;Mukherjee et al, 2021;Stoll et al, 2012). The studies presented in Table 1 present pristine river water monitoring data in which antibiotics and ARGs were detected (or their risk quotient was calculated).…”

Section: Main Antibiotics and Args In Riversmentioning

confidence: 99%

Antimicrobial Resistance in Rivers: A Review of the Genes Detected and New Challenges

Grenni

2022

Enviro Toxic and Chemistry

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River ecosystems are very important parts of the water cycle and an excellent habitat, food, and drinking water source for many organisms, including humans. Antibiotics are emerging contaminants which can enter rivers from various sources. Several antibiotics and their related antibiotic resistance genes (ARGs) have been detected in these ecosystems by various research programs and could constitute a substantial problem. The presence of antibiotics and other resistance cofactors can boost the development of ARGs in the chromosomes or mobile genetic elements of natural bacteria in rivers. The ARGs in environmental bacteria can also be transferred to clinically important pathogens. However, antibiotics and their resistance genes are both not currently monitored by national or international authorities responsible for controlling the quality of water bodies. For example, they are not included in the contaminant list in the European Water Framework Directive or in the US list of Water-Quality Benchmarks for Contaminants. Although ARGs are naturally present in the environment, very few studies have focused on non-impacted rivers to assess the background ARG levels in rivers, which could provide some useful indications for future environmental regulation and legislation. The present study reviews the antibiotics and associated ARGs most commonly measured and detected in rivers, including the primary analysis tools used for their assessment. In addition, other factors that could enhance antibiotic resistance, such as the effects of chemical mixtures, the effects of climate change, and the potential effects of the coronavirus disease 2019 pandemic, are discussed.

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Dissemination of antibiotic resistance genes, mobile genetic elements, and efflux genes in anthropogenically impacted riverine environments

Cited by 28 publications

References 44 publications

Occurrence and driving mechanism of antibiotic resistance genes in marine recreational water around Qinhuangdao, China

Occurrence and driving mechanism of antibiotic resistance genes in marine recreational water around Qinhuangdao, China

Prevalence of antibiotic-resistant Gram-negative bacteria having extended-spectrum β-lactamase phenotypes in polluted irrigation-purpose wastewaters from Indian agro-ecosystems

Antimicrobial Resistance in Rivers: A Review of the Genes Detected and New Challenges

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