Dissemination of a multidrug resistant CTX-M-65 producer Salmonella enterica serovar Infantis clone between marketed chicken meat and children

Martínez-Puchol, Sandra; Riveros, Maribel; Ruidias, Kenny; Granda, Ana Ernestina; Ruiz-Roldán, Lidia; Zapata-Cachay, Cristhian; Ochoa, Theresa J.; Pons, María J.; Ruiz, Joaquı́m

doi:10.1016/j.ijfoodmicro.2021.109109

Cited by 26 publications

(21 citation statements)

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“…Studies regarding the identification and characterization of integrons in Salmonella enterica in this region are scarce. In Peru and Colombia, class 1 and 2 integrons have been detected in multidrug-resistant isolates of Salmonella enterica [18][19][20]. No reports were found from Ecuador and Bolivia.…”

Section: Discussionmentioning

confidence: 99%

“…Thus, the identification and characterization of integrons seem crucial for assessing the epidemiology of antibiotic resistance genes, especially in areas where information is scarce. Class 1 and 2 integrons have been detected in multidrug-resistant isolates of Salmonella enterica reported in Peru and Colombia [18][19][20], while no reports were found from Ecuador and Bolivia. The lack of information regarding integrons limits the capacity to study the dynamics of these elements and their association with antibiotic resistant genes.…”

Section: Introductionmentioning

confidence: 96%

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In Silico Detection of Antimicrobial Resistance Integrons in Salmonella enterica Isolates from Countries of the Andean Community

et al. 2021

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Antimicrobial resistance genes are often associated with integrons, which promote their movement between and within DNA molecules. IntFinder 1.0 and I-VIP v1.2 were used for the detection of integrons and their associated resistance genes in assembled sequences and raw reads. A dataset comprising 1688 sequenced Salmonella enterica isolates from countries of the Andean Community was developed. A total of 749 and 680 integrons were identified by IntFinder 1.0 and I-VIP v1.2, respectively; class 2 integrons were the most abundant followed by class 1, whereas no class 3 integrons were detected. These elements were mainly associated with isolates from animal sources. S. Infantis ST32 contained the majority of integrons. Trimethoprim resistance genes (dfrA) were found in greater numbers than others, including aadA and bla genes. The presence of these resistance integrons may come as a response to antibiotic misuse, especially of co-trimoxazole. This represents a public health risk as novel resistant strains might appear due to gene dissemination. The information gathered from in silico studies not only contributes to our understanding of integron dynamics in pathogenic Salmonella, but also helps identify potential emergent patterns of resistance in the region, which is fundamental for developing pertinent antibiotic surveillance programs.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 96%

In Silico Detection of Antimicrobial Resistance Integrons in Salmonella enterica Isolates from Countries of the Andean Community

et al. 2021

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“…Interestingly, recent genomic studies revealed that the population of S. Infantis is heterogeneous and consists of different clones and clusters (10,11,12). These differences are also evident in the antibiotic resistance profiles being mainly represented by either pan-susceptible or multi-drug resistant strains (4,10,13,14). Multi-drug resistant S.…”

Section: Introductionmentioning

confidence: 99%

Infection dynamics of Salmonella Infantis strains displaying different genetic backgrounds – with or without pESI-like plasmid – vary considerably

Drauch

Kornschober

Palmieri

et al. 2021

Emerging Microbes & Infections

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Food-borne infections with Salmonella are among the most common causes of human diseases worldwide, and infections with the serovar Infantis are becoming increasingly important. So far, diverse phenotypes and genotypes of S . Infantis have been reported. Therefore, the present study aimed to investigate the infection dynamics of two different S . Infantis strains in broilers. For this purpose, 15 birds were infected on day 2 of life with 10 8 CFU/ml of a pESI+ or a pESI– S . Infantis strain, respectively. Ten uninfected birds served as in-contact birds to monitor transmission. In both groups, an increase of infection was observed from 7 days of age onwards, reaching its peak at 28 days. However, the pESI+ strain proved significantly more virulent being re-isolated from most cloacal swabs and organs by direct plating. In contrast, the pESI– strain could be re-isolated from cloacal swabs and caeca only when enrichment was applied. Although the excretion of this strain was limited, the transmission level to in-contact birds was similar to the pESI+ strain. Differences in infection dynamics were also reflected in the antibody response: whereas the pESI+ strain provoked a significant increase in antibodies, antibody levels following infection with the pESI– strain remained in the range of negative control birds. The actual findings provide for the first time evidence of S . Infantis strain-specific infectivity in broilers and confirm previous observations in the field regarding differences in persistence on farms and resistance against disinfectants.

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“…In recent years there has been a rapid increase in bacterial resistance in Brazil and worldwide, the occurrence of this phenomenon in regions such as the Brazilian Amazon is still poorly known, but they are already observed in populations in northern South America in hospital cases and in localized outbreaks [1,2]. The production of Extended Spectrum Β-Lactamase (ESBLs) is an important mechanism of resistance in Enterobacteria.…”

Section: Introductionmentioning

confidence: 99%

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2021

GMR

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In recent years there has been a rapid increase in bacterial resistance in Brazil and worldwide. Among the mechanisms described, Extended-Spectrum ß-Lactamases (ESBL) are of great importance. The continuous assessment of bacterial resistance have fundamental importance in preventing the occurrence of outbreaks and the spread of resistance, especially in the Amazon due to the relative isolation of cities and difficulties in epidemiological surveillance in the region. In the present work we approach the use of phenotypic and genotypic characterization in Enterobacterial isolates by determining the resistance profile to β-lactam antimicrobials by antibiogram and the search for blaCTX-M, blaTEM, blaSHV genes associated with β-lactam resistance. Thus, 88 samples from outpatients from 14 regions in northern Brazil, belonging to the Brazilian Amazon, were analyzed from 2012 to 2014. Of these, Salmonella Typhi (57/64.77%), Salmonella spp. (26/29.54%), Shigella flexneri (2/2.27%), Shigella dysenterie (1/1.14%), Shigella sonnei (1/1.14%) and diarrhogenic Escherichia coli (1/1.14%). As for the resistance profile, only Escherichia coli showed resistance to first, second, third and fourth generation penicillins and cephalosporins and the aztreonam monobactamide, the other isolates did not show resistance profile according to the Clinical and Laboratory Standards (CLSI) standards. The evaluation of the blaCTX-M, blaTEM and blaSHV genes showed positive results only for the blaCTX-M gene in the Escherichia coli sample, with no amplifications being observed in the other analyzed samples. Although resistance was observed in only one sample, the data suggest the circulation of β-lactam resistance genes in Enterobacteria and, therefore, we suggest that continuous resistance evaluation can prevent the spread of β-lactamase-producing Enterobacteria, evidencing the importance of continuous monitoring and efficient strategies to reduce the spread of these agents by preventing outbreaks by resistant strains.

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Dissemination of a multidrug resistant CTX-M-65 producer Salmonella enterica serovar Infantis clone between marketed chicken meat and children

Cited by 26 publications

References 53 publications

In Silico Detection of Antimicrobial Resistance Integrons in Salmonella enterica Isolates from Countries of the Andean Community

In Silico Detection of Antimicrobial Resistance Integrons in Salmonella enterica Isolates from Countries of the Andean Community

Infection dynamics of Salmonella Infantis strains displaying different genetic backgrounds – with or without pESI-like plasmid – vary considerably

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