Dissection of RNAi-based antiviral immunity in plants

Yang, Zhirui; Li, Yang

doi:10.1016/j.coviro.2018.08.003

Cited by 117 publications

(104 citation statements)

References 90 publications

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“…dsRNA is a common pattern formed during replication of RNA and DNA viruses (Paudel and Sanfaçon, 2018;Pumplin and Voinnet, 2013;Ramesh et al, 2017). Antiviral RNA silencing is initiated by cytosolic dicer-like proteins, which bind and cleave the dsRNA into short-interfering RNAs (siRNA) (Guo et al, 2019;Yang and Li, 2018). Subsequently, the siRNAs are loaded into ARGONAUTE (AGO)-containing RNA-induced silencing complexes (RISC), which then target the same or other viral RNA molecules for sequence-specific slicing and/or translational repression.…”

Section: Introductionmentioning

confidence: 99%

Perception of double‐stranded RNA in plant antiviral immunity

Niehl

Heinlein

2019

Molecular Plant Pathology

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Summary RNA silencing and antiviral pattern‐triggered immunity (PTI) both rely on recognition of double‐stranded (ds)RNAs as defence‐inducing signals. While dsRNA recognition by dicer‐like proteins during antiviral RNA silencing is thoroughly investigated, the molecular mechanisms involved in dsRNA perception leading to antiviral PTI are just about to be untangled. Parallels to antimicrobial PTI thereby only partially facilitate our view on antiviral PTI. PTI against microbial pathogens involves plasma membrane bound receptors; however, dsRNAs produced during virus infection occur intracellularly. Hence, how dsRNA may be perceived during this immune response is still an open question. In this short review, we describe recent discoveries in PTI signalling upon sensing of microbial patterns and endogenous ‘danger’ molecules with emphasis on immune signalling‐associated subcellular trafficking processes in plants. Based on these studies, we develop different scenarios how dsRNAs could be sensed during antiviral PTI.

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Section: Introductionmentioning

confidence: 99%

Perception of double‐stranded RNA in plant antiviral immunity

Niehl

Heinlein

2019

Molecular Plant Pathology

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“…In the context of RNA viruses doublestranded (ds) RNA molecules formed during virus replication are recognized and cleaved by Dicer-like proteins (DCL) into small interfering RNAs (siRNAs). siRNAs are loaded into the RNA-induced silencing complex containing members of the ARGONAUTE (AGO) protein family, which directs it to specifically degrade complementary viral RNAs (Baulcombe, 2005;Mlotshwa et al, 2008;Alvarado & Scholthof, 2009;Ding, 2010;Yang & Li, 2018;Guo et al, 2019). Host RNA-dependent RNA polymerases (RDRs) also play an important role in antiviral RNA silencing by amplifying the synthesis of virus-derived dsRNA molecules (Garcia-Ruiz et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Interaction of a plant virus protein with the signature Cajal body protein coilin facilitates salicylic acid‐mediated plant defence responses

et al. 2019

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Summary In addition to well‐known roles in RNA metabolism, the nucleolus and Cajal bodies (CBs), both located within the nucleus, are involved in plant responses to biotic and abiotic stress. Previously we showed that plants in which expression of the CB protein coilin is downregulated are more susceptible to certain viruses including tobacco rattle virus (TRV), suggesting a role of coilin in antiviral defence. Experiments with coilin‐deficient plants and the deletion mutant of the TRV 16K protein showed that both 16K and coilin are required for restriction of systemic TRV infection. The potential mechanisms of coilin‐mediated antiviral defence were elucidated via experiments involving co‐immunoprecipitation, use of NahG transgenic plants deficient in salicylic acid (SA) accumulation, measurement of endogenous SA concentrations and assessment of SA‐responsive gene expression. Here we show that TRV 16K interacts with and relocalizes coilin to the nucleolus. In wild‐type plants these events are accompanied by activation of SA‐responsive gene expression and restriction of TRV systemic infection. By contrast, viral systemic spread was enhanced in NahG plants, implicating SA in these processes. Our findings suggest that coilin is involved in plant defence, responding to TRV infection by recognition of the TRV‐encoded 16K protein and activating SA‐dependent defence pathways.

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“…The C4 protein from Beet severe curly top virus (BSCTV), on the other hand, can interact with SlCLV1, and this has been suggested to potentially underpin symptom development (Li et al, 2018); however, the functional relevance of this interaction is still poorly understood. Interestingly, C4 from yet another geminivirus, Beet curly top virus (BCTV), was previously found to bind the Arabidopsis RLK RGF INSENSITIVE 1 (AtRGI1) (Piroux et al, 2007), although this interaction was not characterized further.…”

Section: Receptor-like Kinases Targeted By Viral Proteinsmentioning

confidence: 99%

“…1). Interestingly, recent works have unveiled that a number of RLKs indeed influence plant susceptibility to viruses, in some cases being direct targets of viral proteins (Korner et al, 2013;Zorzatto et al, 2015;Gouveia et al, 2016;Niehl et al, 2016;Calil and Fontes, 2017;Carluccio et al, 2018;Li et al, 2018;Rosas-Diaz et al, 2018); some of these RLKs are well-known players in plant immunity, while others may exert novel functions in antiviral defence (Fig. 2).…”

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confidence: 99%

Molecular dialogues between viruses and receptor‐like kinases in plants

Macho

Lozano‐Durán

2019

Molecular Plant Pathology

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Summary Receptor‐like kinases (RLKs) play a prominent role in the interaction between plants and extracellular pathogens. Intriguingly, in the past few years several studies have demonstrated that a number of RLKs influence plant susceptibility to viruses and, in some cases, interact with viral proteins. In this review, we will summarize and discuss recent advances suggesting a direct role for RLKs in plant–virus interactions.

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Dissection of RNAi-based antiviral immunity in plants

Cited by 117 publications

References 90 publications

Perception of double‐stranded RNA in plant antiviral immunity

Perception of double‐stranded RNA in plant antiviral immunity

Interaction of a plant virus protein with the signature Cajal body protein coilin facilitates salicylic acid‐mediated plant defence responses

Molecular dialogues between viruses and receptor‐like kinases in plants

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