Dissection and slide mounting technique for male and female genitalia of <i>Leucinodes orbonalis</i>

Padwal, Kanchan G.; Sharma, Sujeet Kumar; Singh, Sameer Kumar

doi:10.5958/0974-4576.2018.00043.9

Cited by 3 publications

(2 citation statements)

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“…The setal arrangement on the prothoracic segment of larvae was studied following Ranjith and Chellappan (2015), and compared with the illustrations of setal arrangements of H. armigera by Passoa (2014). The method suggested by Padwal et al (2018) was followed with minor modifications for dissection of genitalia from both male and female moths and preparation of their slides. All the morphometric measurements are specified based on mean and standard deviation values from 50 randomly selected specimens, using a statistical package (SPSS, Version 16).…”

Section: Methodsmentioning

confidence: 99%

Morphology of Immature Stages and Adults of Helicoverpa Armigera

Chakravarty¹,

Ganguly²,

Padwal³

et al. 2022

ije

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Helicoverpa armigera (Hübner) is a highly polyphagous insect pest of worldwide occurrence, including India. In the present study, a detailed morphological assessment of H. armigera showed the following: typically dome-shaped egg with ribbed surface; larva having coriaceous skin, biordinal crochets and 11 primary setae on the prothoracic segment; pupa adecticous and obtect with prominent posterior tip cremaster; and adult forewings characterized by the presence of 7-8 black spots along the apical margin and a reniform shaped brown marking, more prominent on the underside. Adults exhibit sexual dimorphism in the colour of vestiture and forewings. The genitalic traits like the presence of usually 12 or less sets of cornuti in aedeagus, apically broadened harpe having length ranging between 4.5 to 4.9 mm and a single lobe at the base of everted vesica in males, and four distinct signa on bursa copulatrix in females, also distinguishes it from other congeneric species.

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Section: Methodsmentioning

confidence: 99%

Morphology of Immature Stages and Adults of Helicoverpa Armigera

Chakravarty¹,

Ganguly²,

Padwal³

et al. 2022

ije

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“…Specimens were photographed using camera (Cannon EOS 550D) using a white sheet of paper as a background. Genitalia dissection was carried out following Padwal et al (6) Genitalia were photographed using Moticam 1080 microscope. The photographs were then processed using Adobe Photoshop 2020.…”

Section: Collection and Preservationmentioning

confidence: 99%

Two New Record of the Genus Ampelophaga (Lepidoptera: Sphingidae) from Different Altitude in Mizoram , North East India

Hmar,

Lalnghahpuii,

Lalronunga

et al. 2023

IJST

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Background/Objectives: Sphingidae are a family of moths belonging to the order Lepidoptera, commonly known as hawk moths or Sphinx moth, named for their hovering, streamlined shape and narrow wings. The current study deals with a detailed description of two species of sphingid moth belonging to the genus Ampelophaga: A. rubiginosa and A. dolichoides from Mizoram, North-east India. Methods: Sphingid moths were collected using light trapping method. A white sheet of cloth was hung vertically between two poles and a mercury vapour lamp (160W) as a light source and Honda TM EP1000 portable generator serves as a power source. Moths attracted to light were hand sampled, and killed using petroleum ether. Collected specimens were stored in butter paper with wings folded vertically. Identification and genitalia dissection was done in the Systematics and Toxicology Laboratory, Department of Zoology, Mizoram University. Findings: From our study we observed that A. dolichoides were more prevalent than A. rubiginosa and were found to inhabit much lower elevation whereas A. rubiginosa were found only in the ranges above 600m asl. Though extensive literature survey showed that A. rubiginosa usually occurs between 1200m and 2200m altitude, the current study documented that A. rubiginosa were also found to occur in altitudinal range between 600m-900m asl thereby extending their previously known altitudinal range. Novelty: Extensive survey of literature yielded no reports on the genus Ampelophaga in Mizoram. The study reported two species of the genus Ampelophaga for the first time from Mizoram with notes on their morphological attributes. This study will add to the diversity of sphingid moth of Mizoram and India as well.

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New method of the preparation of male genitalia of tribe Phycitini (Lepidoptera: Pyralidae) using a slide dryer

Lovtsova

Kochiev²

2022

Int J Trop Insect Sci

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Dissection and slide mounting technique for male and female genitalia of Leucinodes orbonalis

Cited by 3 publications

References 0 publications

Morphology of Immature Stages and Adults of Helicoverpa Armigera

Morphology of Immature Stages and Adults of Helicoverpa Armigera

Two New Record of the Genus Ampelophaga (Lepidoptera: Sphingidae) from Different Altitude in Mizoram , North East India

New method of the preparation of male genitalia of tribe Phycitini (Lepidoptera: Pyralidae) using a slide dryer

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