Dissecting the novel mechanism of reduning injection in treating Coronavirus Disease 2019 (COVID-19) based on network pharmacology and experimental verification

Jia, Shanshan; Luo, Hongyu; Liu, Xinkui; Fan, Xiaotian; Huang, Zhihong; Lu, Shan; Shen, Liangliang; Guo, Siyu; Liu, Yingying; Wang, Zhenzhong; Cao, Liang; Cao, Zhipeng; Zhang, Xinzhuang; Zhou, Wei; Zhang, Jingyuan; Li, Jialin; Wu, Jiarui; Xiao, Wei

doi:10.1016/j.jep.2021.113871

Cited by 25 publications

(23 citation statements)

References 77 publications

(67 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The efficacy of RDN as an anti-pneumonia agent has been demonstrated. A large number of studies have shown that RDN can treat different types of lung injury by inhibiting MAPK, NF-κB and other signaling pathways ( Huang et al, 2019 ; Jia et al, 2021 ; Jiang et al, 2019 ; Tang et al, 2014 ). In this study, we verified that RDN injection can ameliorate LPS-induced acute lung injury and the RDN group extend survival time.…”

Section: Discussionmentioning

confidence: 99%

Re-Du-Ning injection ameliorates LPS-induced lung injury through inhibiting neutrophil extracellular traps formation

et al. 2021

Self Cite

View full text Add to dashboard Cite

Background Acute lung injury (ALI) and acute respiratory distress syndrome (ARDS) are life-threatening diseases and could occur in severe COVID-19 patients. Re-Du-Ning injection (RDN) is a tradition Chinese medicine preparation which has been clinically used for treatment of respiratory diseases including COVID-19. Purpose To elucidate the potential mechanisms of RDN for the treatment of ALI. Methods Female C57BL/6J mice were used to establish ALI model by intraperitoneal injection 10 mg/kg LPS, and RDN injection was intraperitoneally administered with the dose of 5 and 10 mL/kg. The cytokines were measured by ELISA and qPCR. The data related to NETs were analyzed by ELISA, immunofluorescence, Western blotting and network pharmacological approach. Results RDN robustly alleviated LPS-induced ALI. Meanwhile, RDN downregulated the expression of pro-inflammatory cytokines, such as IL-1β, IL-6 and TNF-α. Specifically, RDN treatment inhibited the formation of neutrophil extracellular traps (NETs) and remarkably suppressed the protein of PAD4. The active compound from RDN decreased the phosphorylation of ERK1/2. Conclusion These findings demonstrate that RDN ameliorates LPS-induced ALI through suppressing MAPK pathway to inhibit the formation of NETs.

show abstract

Section: Discussionmentioning

confidence: 99%

Re-Du-Ning injection ameliorates LPS-induced lung injury through inhibiting neutrophil extracellular traps formation

et al. 2021

Self Cite

View full text Add to dashboard Cite

show abstract

“…According to sequence alignment and homology modeling, the critical targets of spike, 3C-like protease (3CLpro), papain-like protease (PLpro), and RNA-dependent RNA polymerase (RdRp) protease share 76%, 96%, 83%, 96% sequence similarity between SARS-CoV and SARS-CoV-2, respectively 85 , 86 , 87 . We collected and summarized TCMs and their ingredients to reveal the specific mechanisms of TCM for the three phases of distinct disease stages of COVID-19 42 , 88 , 89 , 90 , 91 , 92 , 93 , 94 , 95 , 96 , 97 , 98 , 99 , 100 , 101 , 102 , 103 , 104 , 105 , 106 , 107 , 108 , 109 , 110 , 111 , 112 , 113 , 114 , 115 , 116 , 117 , 118 , 119 , 120 , 121 , 122 , 123 , 124 , 125 , 126 , 127 , 128 , 129 , 130 , 131 , 132 , 133 , 134 , 135 , 136 , 137 , 138 , 139 , 140 , 141 , 142 , 143 , 144 , 145 , 146 , 147 , …”

Section: Potential Mechanisms Of Tcm For Covid-19mentioning

confidence: 99%

Traditional Chinese medicine in COVID-19

Lyu

Fan

Xiao

et al. 2021

Acta Pharmaceutica Sinica B

151

118

View full text Add to dashboard Cite

COVID-19 pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has spread across the globe, posing an enormous threat to public health and safety. Traditional Chinese medicine (TCM), in combination with Western medicine (WM), has made important and lasting contributions in the battle against COVID-19. In this review, updated clinical effects and potential mechanisms of TCM, presented in newly recognized three distinct phases of the disease, are summarized and discussed. By integrating the available clinical and preclinical evidence, the efficacies and underlying mechanisms of TCM on COVID-19, including the highly recommended three Chinese patent medicines and three Chinese medicine formulas, are described in a panorama. We hope that this comprehensive review not only provides a reference for health care professionals and the public to recognize the significant contributions of TCM for COVID-19, but also serves as an evidence-based in-depth summary and analysis to facilitate understanding the true scientific value of TCM.

show abstract

“…Chinese herbal medicine aimed at the surface proteins or host receptors of these viruses can effectively “keep the enemy out of the country”. For example, RDNI acts on ACE2 to inhibit SARS-CoV-2 invasion, and effectively blocks viral replication in cells by inhibiting the main protease, resulting in a dual-target protective effect ( Jia et al, 2021 ). The active component of LHQWC shared 189 common proteins with ACE2 co-expression proteins, which interact with each other ( Zheng et al, 2020 ) and exert a multi-target synergistic effect that may prevent drug resistance caused by using a single ACE2 inhibitor ( Runfeng et al, 2020 ; Yang et al, 2020 ; Chen et al, 2021a ).…”

Section: Research Status Of Antiviral Tcmsmentioning

confidence: 99%

Progress in Traditional Chinese Medicine Against Respiratory Viruses: A Review

Liu

et al. 2021

Front. Pharmacol.

View full text Add to dashboard Cite

Respiratory viruses, such as severe acute respiratory syndrome coronavirus (SARS-CoV)-1, SARS-CoV-2, influenza A viruses, and respiratory syncytial virus, pose a serious threat to society. Based on the guiding principles of “holism” and “syndrome differentiation and treatment”, traditional Chinese medicine (TCM) has unique advantages in the treatment of respiratory virus diseases owing to the synergistic effect of multiple components and targets, which prevents drug resistance from arising. According to TCM theory, there are two main strategies in antiviral treatments, namely “dispelling evil” and “fu zheng”. Dispelling evil corresponds to the direct inhibition of virus growth and fu zheng corresponds to immune regulation, inflammation control, and tissue protection in the host. In this review, current progress in using TCMs against respiratory viruses is summarized according to modern biological theories. The prospects for developing TCMs against respiratory viruses is discussed to provide a reference for the research and development of innovative TCMs with multiple components, multiple targets, and low toxicity.

show abstract

Dissecting the novel mechanism of reduning injection in treating Coronavirus Disease 2019 (COVID-19) based on network pharmacology and experimental verification

Cited by 25 publications

References 77 publications

Re-Du-Ning injection ameliorates LPS-induced lung injury through inhibiting neutrophil extracellular traps formation

Re-Du-Ning injection ameliorates LPS-induced lung injury through inhibiting neutrophil extracellular traps formation

Traditional Chinese medicine in COVID-19

Progress in Traditional Chinese Medicine Against Respiratory Viruses: A Review

Contact Info

Product

Resources

About