Dissecting the miR-451a-Mif Pathway in Endometriosis Pathophysiology Using a Syngeneic Mouse Model: Temporal Expression of Lesion Mif Receptors, Cd74 and Cxcr4
Abstract:Endometriosis is an enigmatic disease characterized by pain and infertility in which endometrial tissue grows in ectopic locations, predominantly the pelvic cavity. The pathogenesis and pathophysiology of endometriosis is complex and postulated to involve alterations in inflammatory, cell proliferation and post-transcriptional regulatory pathways among others. Our understanding on the pathogenesis and pathophysiology of endometriosis is further complicated by the fact that endometriosis can only be diagnosed b… Show more
“…Laparoscopic surgery, a signi cant advancement in modern science, has emerged as a new option for curative resection of colorectal cancer [67, 99,100]. It allows for precise observation of the surrounding tissue of the lesion, thus minimizing damage [101].…”
Background
Postoperative incision infections are a significant concern in colorectal cancer surgery, impacting patient recovery and well-being. Identification of key risk factors for infection following colorectal cancer surgery is crucial for improving patient outcomes.
Methods
A meta-analytical approach was employed to analyze studies published from January 2015 to December 2022, focusing on variables such as body mass index, diabetes, albumin levels, malnutrition, and surgical duration to assess their association with postoperative infection incidence in colorectal cancer patients.
Results
Analysis of eleven high-quality studies revealed that elevated body mass index, diabetes, low albumin levels, malnutrition, and longer surgical durations were linked to an increased risk of postoperative incision infections. Conversely, laparoscopic procedures demonstrated potential for reducing infection risks.
Conclusions
Effective preoperative risk assessment and management are vital in preventing postoperative incision infections in colorectal cancer patients. These findings offer actionable insights for clinicians to optimize patient prognoses and enhance overall quality of life outcomes.
“…Laparoscopic surgery, a signi cant advancement in modern science, has emerged as a new option for curative resection of colorectal cancer [67, 99,100]. It allows for precise observation of the surrounding tissue of the lesion, thus minimizing damage [101].…”
Background
Postoperative incision infections are a significant concern in colorectal cancer surgery, impacting patient recovery and well-being. Identification of key risk factors for infection following colorectal cancer surgery is crucial for improving patient outcomes.
Methods
A meta-analytical approach was employed to analyze studies published from January 2015 to December 2022, focusing on variables such as body mass index, diabetes, albumin levels, malnutrition, and surgical duration to assess their association with postoperative infection incidence in colorectal cancer patients.
Results
Analysis of eleven high-quality studies revealed that elevated body mass index, diabetes, low albumin levels, malnutrition, and longer surgical durations were linked to an increased risk of postoperative incision infections. Conversely, laparoscopic procedures demonstrated potential for reducing infection risks.
Conclusions
Effective preoperative risk assessment and management are vital in preventing postoperative incision infections in colorectal cancer patients. These findings offer actionable insights for clinicians to optimize patient prognoses and enhance overall quality of life outcomes.
“…miRNAs have highly stable structures and play a significant role in regulating various pathological processes during the formation of EMS, they are involved in controlling the adhesion, invasion, apoptosis, angiogenesis, and proliferation of endometriotic cells [185,189,190]. Within the cells, miRNAs directly participate in the post-transcriptional regulation of target genes, while outside the cells, they can bind to proteins or extracellular vesicles, thus avoiding degradation by ribonucleases [165,191].…”
Section: A Possibility Of Using Mirna As a Diagnostic Marker For Endo...mentioning
Association studies investigating miRNA in relation to diseases have consistently shown significant alterations in miRNA expression, particularly within inflammatory pathways, where they regulate inflammatory cytokines, transcription factors (such as NF-κB, STAT3, HIF1α), and inflammatory proteins (including COX-2 and iNOS). Given that endometriosis (EMS) is characterized as an inflammatory disease, albeit one influenced by estrogen levels, it is natural to speculate about the connection between EMS and miRNA. Recent research has indeed confirmed alterations in the expression levels of numerous microRNAs (miRNAs) in both endometriotic lesions and the eutopic endometrium of women with EMS, when compared to healthy controls. The undeniable association of miRNAs with EMS hints at the emergence of a new era in the study of miRNA in the context of EMS. This article reviews the advancements made in understanding the pathological role of miRNA in EMS and its association with EMS-associated infertility. These findings contribute to the ongoing pursuit of developing miRNA-based therapeutics and diagnostic markers for EMS.
“…To date, various rodent models of endometriosis have been established to elucidate its pathophysiology [ 5 , 6 , 7 , 8 ] and to test the efficacy of the treatment [ 9 , 10 , 11 ]. On the other hand, only a few rodent models have been reported to evaluate endometriosis-related adverse perinatal outcomes [ 12 , 13 , 14 , 15 , 16 ].…”
The purpose of this study was to establish a new mouse model of endometriosis that mimics real-world women’s health problems, in which women continue to be affected by endometriosis long before they wish to become pregnant, and to evaluate the impact of “chronic exposure to endometriosis” on perinatal outcome. Endometriosis was established by the intraperitoneal injection of homologous minced mouse uteri. Vehicle was injected for the control. Mating was initiated either 1 or 43 days after disease establishment (Young or Aged studies, respectively). Mice were sacrificed on 18 dpc. The number pups and resorptions were counted and pups’ body weights (BW) were measured, and the endometriosis lesion was identified and weighted. In the Young study, the number of resorptions and BW were comparable between the groups. In the Aged study, the number of resorptions was significantly higher and BW was significantly lower in endometriosis than that in control. The total weight of endometriosis lesion per dam was significantly lower in the Aged compared to the Young endometriosis group; however, not a single mouse was found to have any lesions at all. These results suggest that in addition to the presence of endometriosis per se, “chronic exposure to endometriosis” prior to pregnancy affect perinatal outcomes.
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