Dissecting the Isoform-Specific Roles of FTZ-F1 in the Larval–Larval and Larval–Pupal Ecdyses in Henosepilachna vigintioctopunctata

Wu, Jian‐Jian; Cheng, Min-Di; Ze, Long-Ji; Shen, Chen-Hui; Jin, Lin; Li, Guoqing

doi:10.3390/insects13030228

Cited by 7 publications

(9 citation statements)

References 33 publications

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“…In the present study, we observed that knockdown of Hvcyp18a1 upregulated the expression of the 20‐hydroxyecdysone (20E) receptor gene ( HvEcR ), as well as four 20E response transcripts ( HvE75, HvHR3, HvBrC , and HvαFTZ‐F1 ), in H. vigintioctopunctata . Previous studies have identified these transcripts as 20E response genes in H. vigintioctopunctata (Wu et al, 2021; Wu, Chen, et al, 2022; Wu, Cheng, et al, 2022), Based on our findings, it can be inferred that CYP18A1 potentially plays a role in 20E elimination. Knockdown of Hvcyp18a1 represses the inactivation of 20E during the end of prepupal stage, resulting in elevated levels of 20E response transcripts compared to the control group.…”

Section: Discussionsupporting

confidence: 71%

“…Upon analyzing the RNAi phenotypes of Hvcyp18a1, we observed a striking resemblance to those observed in HvβFTZ-F1 knockdown beetles (Wu, Cheng, et al, 2022). Similar to HvβFTZ-F1 RNAi animals, the larvae depleted of Hvcyp18a1 developed normally until the prepupal stage but displayed pupation defects in H. vigintioctopunctata.…”

Section: Cyp18a1 Deficiency Inhibits Transcription and Release Of βFt...mentioning

confidence: 61%

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Molecular characterization of the cytochrome P450 enzyme CYP18A1 in Henosepilachna vigintioctopunctata

Zhang,

Tan,

Jin

et al. 2024

Arch Insect Biochem Physiol

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In insects, the expression of 20E response genes that initiate metamorphosis is triggered by a pulse of 20‐hydroxyecdysone (20E). The 20E pulse is generated through two processes: synthesis, which increases its level, and inactivation, which decreases its titer. CYP18A1 functions as an ecdysteroid 26‐hydroxylase and plays a role in 20E removal in several representative insects. However, applying 20E degradation activity of CYP18A1 to other insects remains a significant challenge. In this study, we discovered high levels of Hvcyp18a1 during the larval and late pupal stages, particularly in the larval epidermis and fat body of Henosepilachna vigintioctopunctata, a damaging Coleopteran pest of potatoes. RNA interference (RNAi) targeting Hvcyp18a1 disrupted the pupation. Approximately 75% of the Hvcyp18a1 RNAi larvae experienced developmental arrest and remained as stunted prepupae. Subsequently, they gradually turned black and eventually died. Among the Hvcyp18a1‐depleted animals that successfully pupated, around half became malformed pupae with swollen elytra and hindwings. The emerged adults from these deformed pupae appeared misshapen, with shriveled elytra and hindwings, and were wrapped in the pupal exuviae. Furthermore, RNAi of Hvcyp18a1 increased the expression of a 20E receptor gene (HvEcR) and four 20E response transcripts (HvE75, HvHR3, HvBrC, and HvαFTZ‐F1), while decreased the transcription of HvβFTZ‐F1. Our findings confirm the vital role of CYP18A1 in the pupation, potentially involved in the degradation of 20E in H. vigintioctopunctata.

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Section: Discussionsupporting

confidence: 71%

Section: Cyp18a1 Deficiency Inhibits Transcription and Release Of βFt...mentioning

confidence: 61%

Molecular characterization of the cytochrome P450 enzyme CYP18A1 in Henosepilachna vigintioctopunctata

Zhang,

Tan,

Jin

et al. 2024

Arch Insect Biochem Physiol

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“…The H. vigintioctopunctata beetles were reared using fresh potato foliage at the vegetative growth or young tuber stages [ 43 , 44 ]. At this feeding protocol, the ladybirds progressed through four distinct larval instars (3, 2, 2 and 3 days, respectively), prepupal (2 days), pupal (4 days) and adult stages.…”

Section: Methodsmentioning

confidence: 99%

“…The eggplant lady beetle ( Henosepilachna vigintioctopunctata ), a defoliating pest attacking Solanaceae and Cucurbitaceae crops in many Asian countries [ 42 ], only has four instars. Disruption of the 20E signal fails to bring about supernumerary instars or second pupae in H. vigintioctopunctata [ 43 , 44 ]. We accordingly hypothesized that the larval instars in the beetle were fixed.…”

Section: Introductionmentioning

confidence: 99%

Knockdown of Ecdysone-Induced Protein 93F Causes Abnormal Pupae and Adults in the Eggplant Lady Beetle

Chen

Yang

et al. 2022

Biology

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Ecdysone-induced protein 93F (E93) plays triple roles during post-embryonic development in insects whose juvenile instars are more than four. However, it only acts as a specifier of adult structures in Drosophila flies whose larval instars are fixed at three. In this study, we determined the functions of E93 in the eggplant lady beetle (Henosepilachna vigintioctopunctata), which has four larval instars. We uncovered that E93 was abundantly expressed at the prepupal and pupal stages. A precocious inhibition of the juvenile hormone signal by RNA interference (RNAi) of HvKr-h1 or HvHairy, two vital downstream developmental effectors, at the penultimate instar larval stage increased the expression of E93, Conversely, ingestion of JH by the third-instar larvae stimulated the expression of HvKr-h1 but repressed the transcription of either HvE93X1 or HvE93X2. However, disturbance of the JH signal neither drove premature metamorphosis nor caused supernumerary instars. In contrast, depletion of E93 at the third- and fourth-instar larval and prepupal stages severely impaired pupation and caused a larval-pupal mixed phenotype: pupal spines and larval scoli were simultaneously presented on the cuticle. RNAi of E93 at the pupal stage affected adult eclosion. When the beetles had suffered from a dsE93 injection at the fourth-instar larval and pupal stages, a few resultant adults emerged, with separated elytra, abnormally folded hindwings, a small body size and short appendages. Taken together, our results suggest the larval instars are fixed in H. vigintioctopunctata; E93 serves as a repressor of larval characters and a specifier of adult structures during the larval–pupal–adult transition.

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“…Two isoforms of Ftz‐f1 have been described in dipterans like D. melanogaster (Lavorgna et al, 1993) and A. aegypti (Cruz et al, 2009), as well as in some coleopterans and lepidopterans (Wu et al, 2022). However, a single ftz‐f1 has been identified in the genome of the cockroach B. germanica (Harrison et al, 2018), which plays critical functions during the last nymphal moults (Cruz et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Dual function of the transcription factor Ftz‐f1 on oviposition in the cockroach Blattella germanica

Alborzi

Piulachs

2023

Insect Molecular Biology

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The transcription factor Ftz‐f1 has multiple functions in insect development in a spatial‐temporal line. One of these roles is in the insect ovaries, specifically in the regulation of steroidogenic enzymes production. We studied the function of F in Blattella germanica oogenesis, as it shows two moments of high expression in ovaries: before the imaginal moult, and just before ovulation in the adult. Injecting dsftz‐f1 into adult females, either just after the imaginal moult or just prior to choriogenesis, prevented oviposition, with differences between the two approaches. In 3‐day‐old adult females treated with dsftz‐f1 just after the emergence, the expression of ftz‐f1 was not modified, but the steroidogenic genes increased their expression. ftz‐f1 transcript levels in the ovaries of 5‐day‐old dsftz‐f1‐treated females were significantly depleted, and the expression levels of the same steroidogenic genes began to decrease. These results suggest that Ftz‐f1 regulates the expression of steroidogenic genes in B. germanica, with phm possibly being a key target. Ftz‐f1 has a different temporal function in the cytoskeleton of follicular cells of the basal ovarian follicles. Early in the gonadotrophic cycle, Ftz‐f1 promotes the expression of genes related to the cytoskeleton and muscle proteins, while at the end of the cycle it maintains the expression levels of these genes, thus ensuring correct ovulation.

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Dissecting the Isoform-Specific Roles of FTZ-F1 in the Larval–Larval and Larval–Pupal Ecdyses in Henosepilachna vigintioctopunctata

Cited by 7 publications

References 33 publications

Molecular characterization of the cytochrome P450 enzyme CYP18A1 in Henosepilachna vigintioctopunctata

Molecular characterization of the cytochrome P450 enzyme CYP18A1 in Henosepilachna vigintioctopunctata

Knockdown of Ecdysone-Induced Protein 93F Causes Abnormal Pupae and Adults in the Eggplant Lady Beetle

Dual function of the transcription factor Ftz‐f1 on oviposition in the cockroach Blattella germanica

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