“…Y chromosomal STRs are widely used for sexual assault cases, paternity testing, reconstruction of human population history (Adnan et al, 2018), and missing person investigations (Adnan, Ralf, Rakha, Kousouri, & Kayser, 2016) because of its male inheritance (Rakha et al, 2018). Some previous studies have confirmed the resolution of male lineage differentiations can be improved by carefully adding Y-STRs to currently available Y-STRs set, such as Y-filer (Hanson & Ballantyne, 2004, 2007Hedman, Neuvonen, Sajantila, & Palo, 2011;Rodig et al, 2008). A recently developed Goldeneye ® 20Y kit amplification kit (Goldeneye technology Ltd.) can be used to co-amplify 20 Y-STR loci (DYS19, DYS348, DYS385a/b, DYS388, DYS389I, DYS389II, DYS390, DYS392, DYS393, DYS437, DYS439, DYS447, DYS448, DYS458, DYS460, DYS635, Y-GATA-H4, DYS391, and DYS456) with five dyes (Gao et al, 2017).…”