Dissecting sex pheromone communication of Mythimna separata (Walker) in North China from receptor molecules and antennal lobes to behavior

Jiang, Nan-Ji; Tang, Rui; Wu, Han; Xu, Meng; Ning, Chao; Huang, Ling-Qiao; Wang, Chen‐Zhu

doi:10.1016/j.ibmb.2019.103176

Cited by 28 publications

(58 citation statements)

References 64 publications

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“…In this study, we selected four sex pheromone components Z11–16:Ald, Z11–16:OAc, Z11–16:OH, and 16:Ald, consistent with the sex pheromone blend of M. separata identified by Jiang et al (2019) and four pheromone analogs Z9–14:OAc, Z9–16:Ald, Z9–14:Ald, and Z9–14:OH, to focus on the sex pheromone recognition mechanism in the peripheral neuron system of M. separata . Firstly, we measured electroantennography (EAG) responses of male and female M. separata antennae to sex pheromone components.…”

Section: Introductionmentioning

confidence: 99%

“…Different geographical populations of M. separata have different compositions and proportions in the sex pheromone gland ( Takahashi et al, 1979 ; Zhu et al, 1987 ; Kou et al, 1992 ; Wang and Liu, 1997 ; Lebedeva et al, 2000 ; Fónagy et al, 2011 ; Song et al, 2017 ). The sex pheromone component of female M. separata in north China is a blend of ( Z )-11-hexadecen-1-ol (Z11–16:Ald), ( Z )-11-hexadecen-1-ol (Z11–16:OH), ( Z )-11-hexadecenyl acetate (Z11–16:OAc), and hexadecenal (16:Ald; Jiang et al, 2019 ). Field trapping experiments showed that Z11–16:Ald alone resulted in high male moth captures ( Wei and Pan, 1985 ; Zhu et al, 1987 ; Jung et al, 2013 ; Chen et al, 2018 ).…”

Section: Introductionmentioning

confidence: 99%

“…Field trapping experiments showed that Z11–16:Ald alone resulted in high male moth captures ( Wei and Pan, 1985 ; Zhu et al, 1987 ; Jung et al, 2013 ; Chen et al, 2018 ). A subsequent wind tunnel experiment indicated that single component Z11–16:Ald could sufficiently induce male sexual behaviors and elicit electrophysiological activity of male antennae in the gas chromatography-electroantennographic detection analyses ( Jiang et al, 2019 ). This result revealed that Z11–16:Ald was the major component while the other three were minor components of sex pheromones in geographical populations of M. separata in north China.…”

Section: Introductionmentioning

confidence: 99%

“…And three dendrites were observed in the TS by the transmission electron microscopy (TEM), indicated three neurons housed in TS ( Chang X. Q. et al, 2015 ). Recently, several studies have identified multiple chemosensory genes in the M. separata antennal transcriptome ( Chang et al, 2017 ; He et al, 2017 ; Du et al, 2018 ; Jiang et al, 2019 ). In the geographic population of Kyoto, two PRs were deorphanized and one of them, MsOR1, was mainly tuned to sex pheromone component Z11–16:OAc ( Mitsuno et al, 2008 ).…”

Section: Introductionmentioning

confidence: 99%

“…These genes were named MsepPRs , of which MsepPR1 , MsepPR3 , and MsepPR4 appear to be specifically expressed in male antennae, while MsepPR5 was highly expressed in female antennae ( Du et al, 2018 ). Recent work published by Jiang et al (2019) indicated that the major sex pheromone component Z11–16:Ald activated MsepOR3 and the cumulus of the macroglomerular complex (MGC) in the central nervous system, also studied olfactory coding of sex pheromones in the males M. separata . However, the function of peripheral neurons in discriminating minor sex pheromone components and pheromone analogs is still unclear.…”

Section: Introductionmentioning

confidence: 99%

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Functional Characterization of Sex Pheromone Neurons and Receptors in the Armyworm, Mythimna separata (Walker)

Wang

2021

Front. Neuroanat.

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Pheromone receptors (PRs) of moths are expressed on the dendritic membrane of odorant receptor neurons (ORNs) housed in the long trichoid sensilla (TS) of antennae and are essential to sex pheromone reception. The function of peripheral neurons of Mythimna separata in recognizing sex pheromones is still unclear. In this study, electroantennogram recordings were performed from male and female antennae of M. separata, and showed that the major component of sex pheromones, (Z)-11-hexadecenal (Z11–16:Ald), evoked the strongest response of male antennae with significant differences between sexes. Single sensillum recording was used to record responses of neurons housed in TS of male M. separata. The results revealed four types of TS with three neurons housed in each type, based on profiles of responses to sex pheromone components and pheromone analogs. ORN-B of type-I TS was specifically tuned to the major sex pheromone component Z11–16:Ald; ORN-Bs in type-III and type-IV TSs were, respectively, activated by minor components (Z)-11-hexadecen-1-yl acetate (Z11–16:OAc) and hexadecenal (16:Ald); and ORNs in type-II TS were mainly activated by the sex pheromone analogs. We further cloned full-length sequences of six putative PR genes and an Orco gene. Functional characterization of PRs in the Xenopus oocyte system demonstrated that male antennae-biased MsepPR1 responded strongly to (Z)-9-tetradecenal (Z9-14:Ald), suggesting that MsepPR1 may be expressed in type-II TS. MsepPR6 was exclusively tuned to (Z)-9-tetradecen-1-yl acetate (Z9–14:OAc). MsepPR2 and MsepPR4 showed no responses to any tested components. Female antennae-biased MespPR5 was broadly tuned to Z9–14:Ald, Z9–14:OAc, Z11–16:Ald, and (Z)-11-hexadecen-1-ol (Z11–16:OH). Our results further enriched the sex pheromone recognition mechanism in the peripheral nervous system of moth M. separata.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Functional Characterization of Sex Pheromone Neurons and Receptors in the Armyworm, Mythimna separata (Walker)

Wang

2021

Front. Neuroanat.

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Functional analysis of pheromone receptor repertoire in the fall armyworm, Spodoptera frugiperda

Guo

Gong

et al. 2022

Pest Management Science

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BACKGROUND The fall armyworm, Spodoptera frugiperda (J. E. Smith), is a polyphagous moth species that is spreading all around the globe. It uses (Z)‐9‐tetradecenyl acetate (Z9‐14:Ac) and (Z)‐7‐dodecenyl acetate (Z7‐12:Ac) (100:3.9) as essential sex pheromone components. However, our understanding of the molecular basis of pheromone detection of S. frugiperda is still incomplete. RESULTS Herein, we identified six PRs, i.e. SfruOR6, 11, 13, 16, 56, and 62, by transcriptome sequencing. Subsequently, we heterologously expressed them in Drosophila OR67d neurons and determined their response spectra with a large panel of sex pheromones and analogs. Among them, SfruOR13‐expressing neurons strongly respond to the major sex pheromone component Z9‐14:Ac, but also comparably to (Z,E)‐9,12‐tetradecadienyl acetate (Z9,E12‐14:Ac) and weakly to (Z)‐9‐dodecenyl acetate (Z9‐12:Ac). Both SfruOR56 and SfruOR62 are specifically tuned to the minor sex pheromone component Z7‐12:Ac with varying intensities and sensitivities. In addition, SfruOR6 is activated only by Z9,E12‐14:Ac, and SfruOR16 by both (Z)‐9‐tetradecenol (Z9‐14:OH) and (Z)‐9‐tetradecenal (Z9‐14:Ald). However, the OR67d neurons expressing SfruOR11 remain silent to all compounds tested, a phenomenon commonly found in the OR11 clade of Noctuidae species. Next, using single sensillum recording, we characterized four sensilla types on the antennae of males, namely A, B, C and D types that are tuned to the ligands of PRs, thereby confirming that S. frugiperda uses both SfruOR56 and SfruOR62 to detect Z7‐12:Ac. Finally, using wind tunnel assay, we demonstrate that both Z9,E12‐14:Ac and Z9‐14:OH act as antagonists to the sex pheromone. CONCLUSION We have deorphanized five PRs and characterized four types of sensilla responsible for the detection of pheromone compounds, providing insights into the peripheral encoding of sex pheromones in S. frugiperda.

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Optimization of a pheromone lure by analyzing the peripheral coding of sex pheromones of Spodoptera frugiperda in China

Wang

Zhang

Guo

et al. 2022

Pest Management Science

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BACKGOUND Sex pheromones of the fall armyworm, Spodoptera frugiperda, show differences in composition and proportions in different geographical populations, but always contain Z9‐14:OAc as the major component. Odorant receptor neurons (ORNs) housed in the long trichoid sensilla (TS) of male antennae are essential to detect female‐released sex pheromones in moths. RESULTS In this study, we identified seven components from pheromone gland extracts of female S. frugiperda in the Yunnan population from China, including (Z)‐7‐dodecen‐1‐yl acetate (Z7‐12:OAc), (Z)‐9‐tetradecenal (Z9‐14:Ald), (Z)‐9‐dodecen‐1‐yl acetate (Z9‐12:OAc), (Z)‐9‐tetradecen‐1‐yl acetate (Z9‐14:OAc), (E)‐11‐tetradecen‐1‐yl acetate (E11‐14:OAc), (Z)‐11‐tetradecen‐1‐yl acetate (Z11‐14:OAc) and (Z)‐11‐hexadecen‐1‐yl acetate (Z11‐16:OAc) at a ratio of 1.2:4:0.8:79.1:1.6:1.6:11.7 by gas chromatography coupled with mass spectrometry. Gas chromatography‐electroantennographic detection showed that Z9‐14:OAc, Z7‐12:OAc and Z11‐16:OAc are the male antennal active components. Peripheral coding of pheromones in males was investigated by single sensillum recording. Five functional neurons housed in three types of TS were identified based on profiles of neuronal responses, which are responsible for attractive component Z9‐14:OAc, synergistic components Z7‐12:OAc, Z11‐16:OAc, interspecific pheromones (Z)‐9‐tetradecen‐1‐ol (Z9‐14:OH) and (Z,E)‐9,12‐tetradecadien‐1‐yl acetate (Z9,E12‐14:OAc), respectively. Wind tunnel and field tests demonstrated that a ternary combination of Z9‐14:OAc, Z7‐12:OAc and Z11‐16:OAc at a ratio of 88:1:11 shows the strongest attractiveness to males. Conclusion An optimized pheromone blend of Z9‐14:OAc, Z7‐12:OAc and Z11‐16:OAc in an 88:1:11 ratio was identified for monitoring the invasive pest S. frugiperda in China. Five functional ORNs encoding intra‐ and interspecific pheromones were identified in male antennae, of which three neurons encode attractive component Z9‐14:OAc, synergistic components Z7‐12:OAc and Z11‐16:OAc, respectively, and the other two neurons encode interspecific pheromones Z9‐14:OH and Z9,E12‐14:OAc, separately. © 2022 Society of Chemical Industry.

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Dissecting sex pheromone communication of Mythimna separata (Walker) in North China from receptor molecules and antennal lobes to behavior

Cited by 28 publications

References 64 publications

Functional Characterization of Sex Pheromone Neurons and Receptors in the Armyworm, Mythimna separata (Walker)

Functional Characterization of Sex Pheromone Neurons and Receptors in the Armyworm, Mythimna separata (Walker)

Functional analysis of pheromone receptor repertoire in the fall armyworm, Spodoptera frugiperda

Optimization of a pheromone lure by analyzing the peripheral coding of sex pheromones of Spodoptera frugiperda in China

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