Dissecting root proteome of transgenic rice cultivars unravels metabolic alterations and accumulation of novel stress responsive proteins under drought stress

Paul, Soumitra; Gayen, Dipak; Datta, Swapan K.; Datta, Karabi

doi:10.1016/j.plantsci.2015.02.006

Cited by 43 publications

(32 citation statements)

References 36 publications

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“…Total protein of rice seed (2.0 g) was isolated by phenol extraction method according to Paul et al (2015). The total protein concentration was measured using Bradford reagent (Sigma, USA) and quality of protein was checked by running in 1D SDS-PAGE.…”

Section: Methodsmentioning

confidence: 99%

Metabolic Regulation of Carotenoid-Enriched Golden Rice Line

et al. 2016

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Vitamin A deficiency (VAD) is the leading cause of blindness among children and is associated with high risk of maternal mortality. In order to enhance the bioavailability of vitamin A, high carotenoid transgenic golden rice has been developed by manipulating enzymes, such as phytoene synthase (psy) and phytoene desaturase (crtI). In this study, proteome and metabolite analyses were carried out to comprehend metabolic regulation and adaptation of transgenic golden rice after the manipulation of endosperm specific carotenoid pathways. The main alteration was observed in carbohydrate metabolism pathways of the transgenic seeds. The 2D based proteomic studies demonstrated that carbohydrate metabolism-related enzymes, such as pullulanase, UDP-glucose pyrophosphorylase, and glucose-1-phosphate adenylyltransferase, were primarily up-regulated in transgenic rice seeds. In addition, the enzyme PPDK was also elevated in transgenic seeds thus enhancing pyruvate biosynthesis, which is the precursor in the carotenoids biosynthetic pathway. GC-MS based metabolite profiling demonstrated an increase in the levels of glyceric acid, fructo-furanose, and galactose, while decrease in galactonic acid and gentiobiose in the transgenic rice compared to WT. It is noteworthy to mention that the carotenoid content, especially β-carotene level in transgenic rice (4.3 μg/g) was significantly enhanced. The present study highlights the metabolic adaptation process of a transgenic golden rice line (homozygous T4 progeny of SKBR-244) after enhancing carotenoid biosynthesis. The presented information would be helpful in the development of crops enriched in carotenoids by expressing metabolic flux of pyruvate biosynthesis.

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Section: Methodsmentioning

confidence: 99%

Metabolic Regulation of Carotenoid-Enriched Golden Rice Line

et al. 2016

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“…The extra production of ROS in cells leads to a disruption of the balance between production and removal of ROS which eventually leads to oxidative stress [13]. Paul et al [14] stated that the major class of identified proteins resulting from salinity stress response belongs to carbohydrate and energy metabolism category while stress and defense related proteins are especially up-accumulated under drought stress and a novel protein, “R40C1”, was reported to be up-accumulated in roots of transgenic plants which may play an important role in generation of drought tolerant plants.…”

Section: Introductionmentioning

confidence: 99%

Proteome Analysis of Date Palm (Phoenix dactyliferaL.) under Severe Drought and Salt Stress

Rabey

Al-Malki

Abulnaja

2016

International Journal of Genomics

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Date palm cultivars differently tolerate salinity and drought stress. This study was carried out to study the response of date palm to severe salinity and drought based on leaf proteome analysis. Eighteen-month-old date palm plants were subjected to severe salt (48 g/L NaCl) and drought (82.5 g/L PEG or no irrigation) conditions for one month. Using a protein 2D electrophoresis method, 55 protein spots were analyzed using mass spectrometry. ATP synthase CF1 alpha chains were significantly upregulated under all three stress conditions. Changes in the abundance of RubisCO activase and one of the RubisCO fragments were significant in the same spots only for salt stress and drought stress with no irrigation, and oxygen-evolving enhancer protein 2 was changed in different spots. Transketolase was significantly changed only in drought stress with PEG. The expression of salt and drought stress genes of the chosen protein spots was either overexpressed or downexpressed as revealed by the high or low protein abundance, respectively. In addition, all drought tolerance genes due to no irrigation were downregulated. In conclusion, the proteome analysis of date palm under salinity and drought conditions indicated that both salinity and drought tolerance genes were differentially expressed resulting in high or low protein abundance of the chosen protein spots as a result of exposure to drought and salinity stress condition.

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“…Recent proteomic studies on drought response of crops encompass different plant species, such as wheat [138,157], rice [158,159], barley [160], brassica [154], legumes [141,161]. Different types of tissues are studied: roots [141,154,159], root nodules [161], leaves [138,160], roots and leaves compared at seedling stage [157], roots, flag leaves and spikelets at reproductive stage [151].…”

Section: Proteomics In Search Of Molecular Markers For Assisted Selecmentioning

confidence: 99%

“…Different types of tissues are studied: roots [141,154,159], root nodules [161], leaves [138,160], roots and leaves compared at seedling stage [157], roots, flag leaves and spikelets at reproductive stage [151]. As the high temperature is particularly detrimental during the reproductive stage, besides the studies on leaf proteome under heat stress in rice [113,162], wheat [163] radish [142], alfalfa [164] and stromal proteins in agave [165], special attention is paid to the reproductive phase [166], source-sink interactions at grain filling [167], grain development and composition [155,160] in cereals, and protein composition of soybean seeds [168] formed under unfavorable temperature conditions.…”

Section: Proteomics In Search Of Molecular Markers For Assisted Selecmentioning

confidence: 99%

“…As signal transduction associated, 14-3-3 proteins and calreticulin-like proteins were upregulated in tolerant wheat cultivar under drought stress [157]. In roots of transgenic rice, overexpressing DREB1A, a novel protein containing ricin B lectin domain was found to be highly accumulated, probably with potential role in breeding for drought tolerance [159]. Proteomic findings support and further develop the concept about key mechanisms affected by drought and heat stress and point at some potential protein markers for assisted selection.…”

Section: Proteomics In Search Of Molecular Markers For Assisted Selecmentioning

confidence: 99%

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Selection and Breeding of Suitable Crop Genotypes for Drought and Heat Periods in a Changing Climate: Which Morphological and Physiological Properties Should Be Considered?

2016

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Selection and breeding of genotypes with improved drought/heat tolerance become key issues in the course of global change with predicted increased frequency of droughts or heat waves. Several morphological and physiological plant traits must be considered. Rooting depth, root branching, nutrient acquisition, mycorrhization, nodulation in legumes and the release of nutrients, assimilates or phytohormones to the shoot are relevant in root systems. Xylem embolism and its repair after a drought, development of axillary buds and solute channeling via xylem (acropetal) and phloem (basipetal and acropetal) are key processes in the stem. The photosynthetically active biomass depends on leaf expansion and senescence. Cuticle thickness and properties, epicuticular waxes, stomatal regulation including responses to phytohormones, stomatal plugs and mesophyll resistance are involved in optimizing leaf water relations. Aquaporins, dehydrins, enzymes involved in the metabolism of compatible solutes (e.g., proline) and Rubisco activase are examples for proteins involved in heat or drought susceptibility. Assimilate redistribution from leaves to maturing fruits via the phloem influences yield quantity and quality. Proteomic analyses allow a deeper insight into the network of stress responses and may serve as a basis to identify suitable genotypes, although improved stress tolerance will have its price (often lowered productivity under optimal conditions).

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Dissecting root proteome of transgenic rice cultivars unravels metabolic alterations and accumulation of novel stress responsive proteins under drought stress

Cited by 43 publications

References 36 publications

Metabolic Regulation of Carotenoid-Enriched Golden Rice Line

Metabolic Regulation of Carotenoid-Enriched Golden Rice Line

Proteome Analysis of Date Palm (Phoenix dactyliferaL.) under Severe Drought and Salt Stress

Selection and Breeding of Suitable Crop Genotypes for Drought and Heat Periods in a Changing Climate: Which Morphological and Physiological Properties Should Be Considered?

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