Dissecting promoter of &lt;i&gt;InMYB1&lt;/i&gt; gene showing petal-specific expression

Azuma, Mirai; Oshima, Yoshimi; Sakamoto, Shingo; Mitsuda, Nobutaka; Ohme‐Takagi, Masaru; Otagaki, Shungo; Matsumoto, Shogo; Shiratake, Katsuhiro

doi:10.5511/plantbiotechnology.18.0529a

Cited by 5 publications

(4 citation statements)

References 30 publications

(30 reference statements)

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“…To further evaluate the function of ANAC102 downstream of the JA pathway, we attempted to rescue the dad1 mutant with ANAC102 placed under the control of a heterologous petal-specific promoter from Japanese morning glory, InMYB1 ( InMYB1 pro :AtANAC102 ) 74 – 77 (Supplementary Fig. 24 ).…”

Section: Resultsmentioning

confidence: 99%

Petal abscission is promoted by jasmonic acid-induced autophagy at Arabidopsis petal bases

Furuta,

Yamamoto,

Hirakawa

et al. 2024

Nat Commun

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In angiosperms, the transition from floral-organ maintenance to abscission determines reproductive success and seed dispersion. For petal abscission, cell-fate decisions specifically at the petal-cell base are more important than organ-level senescence or cell death in petals. However, how this transition is regulated remains unclear. Here, we identify a jasmonic acid (JA)-regulated chromatin-state switch at the base of Arabidopsis petals that directs local cell-fate determination via autophagy. During petal maintenance, co-repressors of JA signaling accumulate at the base of petals to block MYC activity, leading to lower levels of ROS. JA acts as an airborne signaling molecule transmitted from stamens to petals, accumulating primarily in petal bases to trigger chromatin remodeling. This allows MYC transcription factors to promote chromatin accessibility for downstream targets, including NAC DOMAIN-CONTAINING PROTEIN102 (ANAC102). ANAC102 accumulates specifically at the petal base prior to abscission and triggers ROS accumulation and cell death via AUTOPHAGY-RELATED GENEs induction. Developmentally induced autophagy at the petal base causes maturation, vacuolar delivery, and breakdown of autophagosomes for terminal cell differentiation. Dynamic changes in vesicles and cytoplasmic components in the vacuole occur in many plants, suggesting JA–NAC-mediated local cell-fate determination by autophagy may be conserved in angiosperms.

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Section: Resultsmentioning

confidence: 99%

Petal abscission is promoted by jasmonic acid-induced autophagy at Arabidopsis petal bases

Furuta,

Yamamoto,

Hirakawa

et al. 2024

Nat Commun

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“…InMYB1 is expressed specifically in petal and is involved in the regulation of petal coloration (anthocyanin accumulation) in morning glory [42]. The promoter of InMYB1 can be used as a petal-specific promoter [73, 74, 75]. INIL00g10723 , which was not mapped to any pseudo-chromosome, but to a scaffold (Fig.…”

Section: Resultsmentioning

confidence: 99%

Genome-wide analysis of R2R3-MYB transcription factors in Japanese morning glory

Komatsuzaki

Hoshino

Shiratake

et al. 2022

Preprint

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The R2R3-MYB transcription factor is one of the largest transcription factor families in plants. R2R3-MYBs play a variety of functions in plants, such as cell fate determination, organ and tissue differentiations, primary and secondary metabolisms, stress and defense responses and other physiological processes. The Japanese morning glory (Ipomoea nil) has been widely used as a model plant for flowering and morphological studies. In the present study, 126 R2R3-MYB genes were identified in the Japanese morning glory genome. Information, including gene structure, protein motif, chromosomal location and gene expression, were assigned to the InR2R3-MYBs . Phylogenetic tree analysis revealed that the 126 InR2R3-MYBs were classified into 29 subfamilies (C1-C29). Herein, physiological functions of the InR2R3-MYBs are discussed based on the functions of their Arabidopsis orthologues. InR2R3-MYBs in C9, C15, C16 or C28 may regulate cell division, flavonol biosynthesis, anthocyanin biosynthesis or response to abiotic stress, respectively. C16 harbors the known anthocyanin biosynthesis regulator, InMYB1 (INIL00g10723), and putative anthocyanin biosynthesis regulators, InMYB2 (INIL05g09650) and InMYB3 (INIL05g09651). In addition, INIL05g09649, INIL11g40874 and INIL11g40875 in C16 were suggested as novel anthocyanin biosynthesis regulators. We organized the R2R3-MYB transcription factors in the morning glory genome and assigned information to gene and protein structures and presuming their functions. Our study is expected to facilitate future research on R2R3-MYB transcription factors in Japanese morning glory.

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“…To further evaluate the function of ANAC102 downstream of the JA pathway, we attempted to rescue the dad1 mutant with ANAC102 placed under the control of a petal-speci c promoter from Japanese morning glory, InMYB1 (pInMYB1::ANAC102) [73][74][75][76] (Extended Data Fig. 14).…”

Section: Ja Mediates Key Ja Homeostasis Targets During Petal Abscissionmentioning

confidence: 99%

Local and terminal cell differentiation mediated by the jasmonic acid-mediated autophagy evokes petal abscission in Arabidopsis thaliana

Yamamoto

Furuta

Hirakawa³

et al. 2023

Preprint

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In angiosperms, transition from floral organ maintenance to abscission determines plant reproductive success and seed dispersion. For petal abscission, local cell fate decision at petal cell base is more important than general senescence or cell death of petals at organ levels. However, how this decision is regulated in a spatiotemporal manner remains unclear. Here, we describe a jasmonic acid (JA)–regulated chromatin state change that directs the local cell fate determination via autophagy at the base of petals. During petal maintenance phase, transcriptional co-repressors of JA signaling accumulate at the base of petals to block MYC activity, leading to lower reactive oxygen species (ROS) levels. JA acts as an airborne signaling molecule transmitted from stamens to petals and accumulates primarily in petal bases to trigger epigenetic reprograming, allowing MYC transcription factors to interact with MEDIATOR25 and to increase chromatin accessibility for key downstream targets, such as Arabidopsis NAC DOMAIN-CONTAINING PROTEIN102 (ANAC102). ANAC102 specifically accumulates at the base of petals just prior to petal abscission and triggers local ROS accumulation and cell death via AUTOPHAGY-RELATED GENES induction. Notably, this ANAC102-triggered autophagy at the petal base causes maturation, vacuolar delivery, and breakdown of autophagosome for terminal cell differentiation. Because dynamic changes in the number of vesicles and cytoplasmic components in the vacuole are often seen in many plants, the local cell fate determination by autophagy may be conserved for petal abscission across angiosperms and may have been recruited under the JA/NAC module to pattern plant organ detachment systems.

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Dissecting promoter of <i>InMYB1</i> gene showing petal-specific expression

Cited by 5 publications

References 30 publications

Petal abscission is promoted by jasmonic acid-induced autophagy at Arabidopsis petal bases

Petal abscission is promoted by jasmonic acid-induced autophagy at Arabidopsis petal bases

Genome-wide analysis of R2R3-MYB transcription factors in Japanese morning glory

Local and terminal cell differentiation mediated by the jasmonic acid-mediated autophagy evokes petal abscission in Arabidopsis thaliana

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