Dissecting postharvest chilling injury through biotechnology

Albornoz, Karin; Zhou, Jiaqi; Yu, Jingwei; Beckles, Diane M.

doi:10.1016/j.copbio.2022.102790

Cited by 35 publications

(21 citation statements)

References 103 publications

(91 reference statements)

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“…All these abnormalities can be linked to specific cellular dysfunctions as summarized by Albornoz et al. (2022) .…”

Section: Introductionmentioning

confidence: 99%

“…Although molecular mechanisms activated during CI have been investigated, there is no recent review that covers the latest multi-omics studies for fleshy fruit of Rosaceae species. The present work aims to review and organize the multi-omics data, focusing on the pome ( Maloideae ) and stone ( Prunoideae ) fruits, as they represent a largely significant part of cultivated fruits that suffer from CI during CS, using as a blueprint the roadmap developed by Albornoz and co-workers (2022) . To effectively implement the model, we have also incorporated information derived from chemical treatments aimed at alleviating CI.…”

Section: Introductionmentioning

confidence: 99%

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Dissecting postharvest chilling injuries in pome and stone fruit through integrated omics

Rodrigues,

Ordoñez-Trejo,

Rasori

et al. 2024

Front. Plant Sci.

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Lowering the storage temperature is an effective method to extend the postharvest and shelf life of fruits. Nevertheless, this technique often leads to physiological disorders, commonly known as chilling injuries. Apples and pears are susceptible to chilling injuries, among which superficial scald is the most economically relevant. Superficial scald is due to necrotic lesions of the first layers of hypodermis manifested through skin browning. In peaches and nectarines, chilling injuries are characterized by internal symptoms, such as mealiness. Fruits with these aesthetic or compositional/structural defects are not suitable for fresh consumption. Genetic variation is a key factor in determining fruit susceptibility to chilling injuries; however, physiological, or technical aspects such as harvest maturity and storage conditions also play a role. Multi-omics approaches have been used to provide an integrated explanation of chilling injury development. Metabolomics in pome fruits specifically targets the identification of ethylene, phenols, lipids, and oxidation products. Genomics and transcriptomics have revealed interesting connections with metabolomic datasets, pinpointing specific genes linked to cold stress, wax synthesis, farnesene metabolism, and the metabolic pathways of ascorbate and glutathione. When applied to Prunus species, these cutting-edge approaches have uncovered that the development of mealiness symptoms is linked to ethylene signaling, cell wall synthesis, lipid metabolism, cold stress genes, and increased DNA methylation levels. Emphasizing the findings from multi-omics studies, this review reports how the integration of omics datasets can provide new insights into understanding of chilling injury development. This new information is essential for successfully creating more resilient fruit varieties and developing novel postharvest strategies.

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“…All these abnormalities can be linked to specific cellular dysfunctions as summarized by Albornoz et al. (2022) .…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Dissecting postharvest chilling injuries in pome and stone fruit through integrated omics

Rodrigues,

Ordoñez-Trejo,

Rasori

et al. 2024

Front. Plant Sci.

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“…Different forms of CI are gradually manifested through the occurrence of cell membrane peroxidation and membrane phase transition. When the permeability of the cell membrane increases, the ion balance of the cell is destroyed, causing the physiological metabolism disorder and the accumulation of harmful substances, which leads to CI symptoms in postharvest crops (Albornoz et al 2022). Phytohormones play an important role in the adaptation of plants to cold environments.…”

Section: Introductionmentioning

confidence: 99%

Transcriptome analysis of harvested okra (Abelmoschus esculentus L.) in response to chilling stress

Zhu

Jian-xiang

Tang

et al. 2023

Preprint

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Okra (Abelmoschus esculentus L.) is a novel vegetable with high nutritional and medicinal values. However, as a cold-sensitive crop, okra pods are susceptible to chilling injury (CI) during cold chain packaging and transportation, and the molecular mechanism of postharvest okra pods in response to chilling stress has not been elucidated. In this study, after storage at 4 °C for 15 d, okra pods from ‘Hokkaido’ exhibited progressively worseningCI symptoms, as well as continuously elevated CI index and CI incidence. Transcriptomic analysis showed that during low-temperature storage, many unigenes were activated by chilling stress and were mainly enriched in ‘Signal transduction’, ‘Amino acid metabolism’, and ‘Carbohydrate metabolism’. Further studies showed that the biosynthesis and signaling mechanism of ethylene (ETH) and abscisic acids (ABA) was activated by chilling stress, which induced reactive oxygen species (ROS) over-accumulation and up-regulated genes related to membrane lipid peroxidation. Chilling stress also activated genes involved in chlorophyll degradation and reduced chlorophyll a, chlorophyll b, and total chlorophyll content. In addition, 156 transcription factors (TFs) belonging to 12 families were identified from transcript databases. This study gained insight into the chilling transcriptional response mechanism of postharvest okra pods, which will contribute to cold chain management and molecular breeding of okra.

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“…Although a longer shelf-life reduces the rate of fruit deterioration and postharvest losses while making produce suitable for commercial sale, the unintended negative effects on quality can lead to rejection by the consumers, creating postharvest waste 1 . Understanding the mechanisms of postharvest-induced changes in tomato through fruit physiology and molecular biology is a first step towards finding a solution for postharvest loss and waste 2 .…”

Section: Introductionmentioning

confidence: 99%

“…Cold storage is used to slow down senescence and preserve quality in harvested fruit by reducing respiration, biochemical reactions, the rate of fungal infestation and water loss 2 . However, tomato and other tropical and subtropical crops are sensitive to low temperatures.…”

Section: Introductionmentioning

confidence: 99%

Integrative analysis of the methylome and transcriptome of tomato fruit (Solanum lycopersicumL.) induced by postharvest handling

Zhou,

Chen

et al. 2023

Preprint

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Tomato fruit ripening is triggered by the demethylation of key genes, which alters their transcriptional levels thereby initiating and propagating a cascade of physiological events. What is unknown, is how these processes are altered when fruit are ripened using postharvest practices to extend shelf-life, as these practices often reduce fruit quality. To address this, postharvest handling-induced changes in the fruit DNA methylome and transcriptome, and how they correlated with ripening speed, and ripening indicators such as ethylene, ABA, and carotenoids, were assessed. This study comprehensively connected changes in physiological events with dynamic molecular changes. Ripening fruit that reached 'Turning' (T) after storage under dark at 20°C, 12.5°C, or 5°C chilling (followed by 20°C rewarming), were compared to fresh-harvest fruit 'FHT'. Fruit stored at 12.5°C, had the biggest epigenetic marks and alterations in gene expression, exceeding changes induced by postharvest chilling. Fruit physiological and chronological age were uncoupled at 12.5°C, as the time-to-ripening was longest. Fruit ripening at 12.5°C was not climacteric; there was no respiratory or ethylene burst, rather, fruit were high in ABA. Clear differentiation between postharvest-ripened and 'FHT' was evident in the methylome and transcriptome. Higher expression of photosynthetic genes and chlorophyll levels in 'FHT' fruit, pointing to light as influencing the molecular changes in fruit ripening. Finally, correlative analyses of the -omics data putatively identified genes regulated by DNA methylation. Collectively these data improve our interpretation of how tomato fruit ripening patterns are altered by postharvest practices, and long-term are expected to help improve fruit quality.

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Dissecting postharvest chilling injury through biotechnology

Cited by 35 publications

References 103 publications

Dissecting postharvest chilling injuries in pome and stone fruit through integrated omics

Dissecting postharvest chilling injuries in pome and stone fruit through integrated omics

Transcriptome analysis of harvested okra (Abelmoschus esculentus L.) in response to chilling stress

Integrative analysis of the methylome and transcriptome of tomato fruit (Solanum lycopersicumL.) induced by postharvest handling

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