Dissecting genomic hotspots underlying seed protein, oil, and sucrose content in an interspecific mapping population of soybean using high‐density linkage mapping

Abstract

SummaryThe cultivated [Glycine max (L) Merr.] and wild [Glycine soja Siebold & Zucc.] soybean species comprise wide variation in seed composition traits. Compared to wild soybean, cultivated soybean contains low protein, high oil, and high sucrose. In this study, an interspecific population was derived from a cross between G. max (Williams 82) and G. soja (PI 483460B). This recombinant inbred line (RIL) population of 188 lines was sequenced at 0.3× depth. Based on 91 342 single nucleotide polymorphisms (SNPs), recombination events in RILs were defined, and a high‐resolution bin map was developed (4070 bins). In addition to bin mapping, quantitative trait loci (QTL) analysis for protein, oil, and sucrose was performed using 3343 polymorphic SNPs (3K‐SNP), derived from Illumina Infinium BeadChip sequencing platform. The QTL regions from both platforms were compared, and a significant concordance was observed between bin and 3K‐SNP markers. Importantly, the bin map derived from next‐generation sequencing technology enhanced mapping resolution (from 1325 to 50 Kb). A total of five, nine, and four QTLs were identified for protein, oil, and sucrose content, respectively, and some of the QTLs coincided with soybean domestication‐related genomic loci. The major QTL for protein and oil were mapped on Chr. 20 (qPro_20) and suggested negative correlation between oil and protein. In terms of sucrose content, a novel and major QTL were identified on Chr. 8 (qSuc_08) and harbours putative genes involved in sugar transport. In addition, genome‐wide association using 91 342 SNPs confirmed the genomic loci derived from QTL mapping. A QTL‐based haplotype using whole‐genome resequencing of 106 diverse soybean lines identified unique allelic variation in wild soybean that could be utilized to widen the genetic base in cultivated soybean.

Quantitative trait loci analysis of seed oil content and composition of wild and cultivated soybean

“…35-35.95 Mb on Chr. Twenty for seed oil content [14,[21][22][23][24][25] and the QTL region of 44.58-48.58 Mb on Chr. Fourteen for seed linolenic acid [11,13,24,26].…”

“…With the completion of the genome sequencing of soybean cv. Williams 82 [29] and the rapid development of next-generation sequencing (NGS) technology, singlenucleotide polymorphism (SNP) markers have been used to construct high-density linkage maps to identify QTL intervals [25,30]. Based on a high-density genetic map consisting of 2062 SNP markers, Cao et al (2017) identified one QTL, qOil-5, for seed oil, which was mapped to Chr.…”

“…05, with a physical distance of 2.5 Mb [30]. Using the Illumina Infinium BeadChip sequencing platform, Patil et al (2018) reported stable QTLs for oil content on Chr. 02 (qOil_02), Chr.…”

“…15 (qOil_ 15), and Chr. 20 (qOil_20) using 3343 polymorphic SNPs (3 K-SNP) [25]. Specific-locus amplified fragment sequencing (SLAF-seq) technology has been used to construct high-density genetic maps, constituting an efficient method for large-scale de novo SNP discovery and genotyping in soybean [31,32].…”

“…Cultivated soybean seeds have an oil content of approximately 18-22%, whereas wild soybean seeds contain about 8-10% oil [25]. In an attempt to identify genes controlling seed oil content and composition, QTL analyses for oil content between cultivated and wild soybean need to be conducted.…”