Dissecting drug and vehicle metabolic effects in rats by a metabonomic approach

Colafranceschi, Mauro; Capuani, Giorgio; Miccheli, Alfredo; Campo, Silvia; Valerio, Mariacristina; Tomassini, Alberta; Giuliani, Alessandro; Arseni, Brunilde; Rossi, Stefania; Santis, Rita De; Carminati, Paolo; Ruggiero, Vito; Conti, Filippo

doi:10.1016/j.jbbm.2006.08.007

Cited by 4 publications

(5 citation statements)

References 16 publications

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“…The combination of pattern recognition methods with assigned signal analysis (ASA) has been reported as a very useful strategy for improving the interpretation of metabonomic data (Colafranceschi et al, 2007;Giuliani, Zbilut, Conti, Manetti, & Miccheli, 2004;Manetti et al, 2006). Thus, the next step was to achieve a semiquantitative analysis through the integration of a characteristic NMR signal for a series of selected metabolites in the original spectrum.…”

Section: Metabolic Analysis Of Tomato Fruit By Means Of 1 H Hrmas Nmrmentioning

confidence: 99%

HRMAS-nuclear magnetic resonance spectroscopy characterization of tomato “flavor varieties” from Almería (Spain)

Pérez

López

Iglesias

et al. 2011

Food Research International

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Section: Metabolic Analysis Of Tomato Fruit By Means Of 1 H Hrmas Nmrmentioning

confidence: 99%

HRMAS-nuclear magnetic resonance spectroscopy characterization of tomato “flavor varieties” from Almería (Spain)

Pérez

López

Iglesias

et al. 2011

Food Research International

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“…Thus, sesame oil may affect social behavior by increasing overall arousal (Pfaff et al, 2008). Alternatively, it is possible that the effects found in the present study are due to metabolic effects of the sesame oil, which have been observed with SC injections in rats (Colafranceschi et al, 2007), although why they would be seen 72 hours after administration is unclear. For the same reason, it is unlikely that these effects are due to the stress of the injection.…”

Section: Note On Vehicle Effectsmentioning

confidence: 74%

“…In addition, in order to assess potential effects of sesame oil on behavior (Anton et al, 1974; Curtis and Wang, 2005; Colafranceschi et al, 2007), an uninjected group was included for each gonadal condition. This also allowed us to compare gonadex and gonadally intact mice in untreated animals.…”

Section: Methodsmentioning

confidence: 99%

“…In the few studies in which an uninjected control group was used, or the effects of sesame oil were directly examined, vehicle or sesame oil effects were found in various species and with different modes of administration (oral, IP, SC; Anton et al, 1974; Curtis and Wang, 2005; Colafranceschi et al, 2007). Given the implications this may have for endocrinological studies, further investigations into these effects are warranted.…”

Section: Note On Vehicle Effectsmentioning

confidence: 99%

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Agonistic behavior in males and females: Effects of an estrogen receptor beta agonist in gonadectomized and gonadally intact mice

Allen

Cragg

Wood

et al. 2010

Psychoneuroendocrinology

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Summary Affiliative and agonistic social interactions are mediated by gonadal hormones. Research with estrogen receptor alpha (ERα) or beta (ERβ) knockout (KO) mice show that long-term inactivation of ERα decreases, while inactivation of ERβ increases, male aggression. Opposite effects were found in female αERKO and βERKO mice. The role of acute activation of ERα or ERβ in the agonistic responses of adult non-KO mice is unknown. We report here the effects of the ERβ selective agonist WAY-200070 on agonistic and social behavior in gonadally intact and gonadectomized (gonadex) male and female CD-1 mice towards a gonadex, same-sex intruder. All 15 min resident-intruder tests were videotaped for comprehensive behavioral analysis. Separate analyses assessed: 1) effects of WAY-200070 on each sex and gonadal condition; 2) differences between sexes, and between gonadally intact and gonadex mice, in untreated animals. Results show that in gonadally intact male and female mice WAY-200070 increased agonistic behaviors such as pushing down and aggressive grooming, while leaving attacks unaffected. In untreated mice, males attacked more than females, and gonadex animals showed less agonistic behavior than same-sex, gonadally intact mice. Overall, our detailed behavioral analysis suggested that in gonadally intact male and female mice, ERβ mediates patterns of agonistic behavior that are not directly involved in attacks. This suggests that specific aspects of aggressive behavior are acutely mediated by ERβ in adult mice. Our results also showed that, in resident-intruder tests, female mice spend as much time in intrasexual agonistic interactions as males, but use agonistic behaviors that involve extremely low levels of direct attacks. This non-attack aggression in females is increased by acute activation of ERβ. Thus, acute activation of ERβ similarly mediates agonistic behavior in adult male and female CD-1 mice.

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“…Metabolomics may provide the missing link between genotype and phenotype (Fiehn 2002), yielding information that is complementary to genomic, transcriptomic and proteomic analysis and that would advance our understanding of pathogenic mechanisms and metabolic phenotype , improve diagnosis (Yang et al 2004;Odunsi et al 2005), help monitor treatment regimens (Lehtimaki et al 2003) and serve as a screening tool for new targeted drugs (Cascante et al 2002;Miccheli et al 2003;Colafranceschi et al 2007). Metabolomics provides a straightforward investigative approach which, as stressed by Bailey, we urgently need to be able to tackle the cell as a 'complex system', identify novel drug targets and, ultimately, revert the current stagnation in the rate of pharmaceutical discovery (Bailey 2001).…”

Section: System Thinkingmentioning

confidence: 99%

Beyond the Oncogene Paradigm: Understanding Complexity in Cancerogenesis

et al. 2008

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In the past decades, an enormous amount of precious information has been collected about molecular and genetic characteristics of cancer. This knowledge is mainly based on a reductionistic approach, meanwhile cancer is widely recognized to be a 'system biology disease'. The behavior of complex physiological processes cannot be understood simply by knowing how the parts work in isolation. There is not solely a matter how to integrate all available knowledge in such a way that we can still deal with complexity, but we must be aware that a deeply transformation of the currently accepted oncologic paradigm is urgently needed. We have to think in terms of biological networks: understanding of complex functions may in fact be impossible without taking into consideration influences (rules and constraints) outside of the genome. Systems Biology involves connecting experimental unsupervised multivariate data to mathematical and computational approach than can simulate biologic systems for hypothesis testing or that can account for what it is not known from high-throughput data sets. Metabolomics could establish the requested link between genotype and phenotype, providing informations that ensure an integrated understanding of pathogenic mechanisms and metabolic phenotypes and provide a screening tool for new targeted drug.

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Dissecting drug and vehicle metabolic effects in rats by a metabonomic approach

Cited by 4 publications

References 16 publications

HRMAS-nuclear magnetic resonance spectroscopy characterization of tomato “flavor varieties” from Almería (Spain)

HRMAS-nuclear magnetic resonance spectroscopy characterization of tomato “flavor varieties” from Almería (Spain)

Agonistic behavior in males and females: Effects of an estrogen receptor beta agonist in gonadectomized and gonadally intact mice

Beyond the Oncogene Paradigm: Understanding Complexity in Cancerogenesis

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