Disruption of virus movement confers broad-spectrum resistance against systemic infection by plant viruses with a triple gene block.

Beck, David L.; Dolleweerd, Craig J. van; Lough, Tony J.; Balmori, Ezequiel; Voot, Davin M.; Andersen, Mark; O'Brien, Iona E.W.; Forster, R. L. S.

doi:10.1073/pnas.91.22.10310

Cited by 90 publications

(28 citation statements)

References 39 publications

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“…It is known that a nonfunctional MP expressed in transgenic plants confers resistance to multiple viruses by inhibiting their cell-to-cell movement (2,11,25,29). The inhibitory effect on virus movement has been considered due to antagonism between competent and incompetent MPs.…”

Section: Discussionmentioning

confidence: 99%

Conversion in the Requirement of Coat Protein in Cell-to-Cell Movement Mediated by the Cucumber Mosaic Virus Movement Protein

Nagano

Mise

Furusawa

et al. 2001

J Virol

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Plant viruses have movement protein (MP) gene(s) essential for cell-to-cell movement in hosts.Cucumber mosaic virus (CMV) requires its own coat protein (CP) in addition to the MP for intercellular movement. Our present results using variants of both CMV and a chimeric Brome mosaic virus with the CMV MP gene revealed that CMV MP truncated in its C-terminal 33 amino acids has the ability to mediate viral movement independently of CP. Coexpression of the intact and truncated CMV MPs extremely reduced movement of the chimeric viruses, suggesting that these heterogeneous CMV MPs function antagonistically. Sequential deletion analyses of the CMV MP revealed that the dispensability of CP occurred when the C-terminal deletion ranged between 31 and 36 amino acids and that shorter deletion impaired the ability of the MP to promote viral movement. This is the first report that a region of MP determines the requirement of CP in cell-to-cell movement of a plant virus.Plant viruses encode proteins that control their movement from cell to cell. These proteins are called movement proteins (MPs) and interact with the normal symplastic connections between plant cells, the plasmodesmata, by modifying the plasmodesmal structure and function. Consequently, the highly regulated passage of small molecules through plasmodesmata is altered to allow the passage of large nucleoprotein complexes containing the viral genome (8, 28).Some viruses, including Tobacco mosaic virus (TMV) and Red clover necrotic mosaic virus, do not require the viral coat protein (CP) for cell-to-cell movement. The MPs of these viruses have a nucleic acid binding activity (10, 37), and an MP-RNA nucleoprotein complex is thought to pass through the modified plasmodesmata to adjacent uninfected cells (8,26). Other viruses do not move from cell to cell in the absence of viral CP. Cauliflower mosaic virus and Cowpea mosaic virus are known to move as virus-like particles through tubules that pass through plasmodesmata into neighboring cells. These tubules are composed of MP (40, 49). Nepo-, Tospo-, and Fabaviruses are also thought to move similarly with the tubulemediated mechanism. There are viruses that are considered to move as a nucleoprotein complex different from virus particles despite their requirement of viral CP for cell-to-cell movement. Cucumber mosaic virus (CMV) is competent to induce tubules protruding from infected protoplasts like the viruses that move as virus-like particles (6). Nevertheless, no such tubules have been found in planta by electron microscopy (3), and mutants incapable of virion formation move successfully from cell to cell (22,44,46). CMV is the type member of the genus Cucumovirus and is one of the most common plant viruses of substantial agricultural significance. CMV infects more than 1,000 species of plants, shrubs, and trees and both monocots and dicots (41). The genomic RNAs of CMV are designated as RNAs 1, 2, and 3, by diminishing size (39). All the RNAs have a cap structure at the 5Ј terminus. The 3Ј portion of all the RNAs is ...

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Section: Discussionmentioning

confidence: 99%

Conversion in the Requirement of Coat Protein in Cell-to-Cell Movement Mediated by the Cucumber Mosaic Virus Movement Protein

Nagano

Mise

Furusawa

et al. 2001

J Virol

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“…Ideally, this resistance should also include protection against alfalfa mosaic virus and clover yellow vein potyvirus which are already established in New Zealand, and other serious viral pathogens of white clover such as clover yellow mosaic potexvirus and peanut stunt cucumovirus, which have not yet become established in New Zealand. The broad spectrum resistance provided by a dysfunctional movement gene of WC1MV (Beck et al 1994) is one example of such resistance.…”

Section: Discussionmentioning

confidence: 99%

“…Therefore, our approach involves genetic engineering for resistance . To date, we have demonstrated resistance to WC1MV in Nicotiana benthamiana plants engineered to express the WC1MV coat protein gene (Beck et al 1993), a dysfunctional WC1MV movement protein gene (Beck et al 1994), and the WC1MV replicase gene (Forster et al 1997). Furthermore, the coat protein gene has been introduced into white clover cv.…”

Section: Introductionmentioning

confidence: 99%

Estimating the agronomic impact of white clover mosaic virus on white clover performance in the North Island of New Zealand

Dudas

Woodfield

Tong

et al. 1998

New Zealand Journal of Agricultural Research

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The agronomic impact of white clover mosaic potexvirus (WC1MV) was determined using yield trials and a survey of virus incidence. The virus was shown to occur at high levels in white clover plants in pastures throughcut the North Island. The virus was detected in 69% of white clover plants from 42 mature pastures examined (>4 years old) and in 52% of white clover plants examined on roadsides. The virus was also detected in 15 pastures less than 4 years old, but at a lower level (18% infection). The effect of WC1MV on plant growth was determined using clonal and non-clonal lines of the modern white clover cv. Grasslands Tahora in a replicated spaced plant field trial. Uninoculated plants were shown to out-produce inoculated plants at each of three harvest dates over one year. Infected plants produced, in total, 36.5% less dry matter than uninoculated plants. The major effect of WC1MV A 97056Received 11 August 1997; accepted 4 December 1997 infection was on stolon elongation, with lesser effects on leaf length and petiole height. These results indicate that substantial gains can be expected from cultivars resistant to this pathogen.

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“…3 and 10; review ref. 4), but cases of proteindependent pathogen-derived resistance due to the expression of viral movement proteins or replicases are also known (9,11,12). In some instances, resistance is based on the expression of intact, functional proteins; in others the expression of the intact protein leads only to weak resistance or even to enhanced susceptibility.…”

mentioning

confidence: 99%

Peptide-mediated broad-spectrum plant resistance to tospoviruses

Rudolph

Schreier

Uhrig

2003

Proc. Natl. Acad. Sci. U.S.A.

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Plant viruses have a significant impact on agronomic losses worldwide. A new strategy for engineering virus-resistant plants by transgenic expression of a dominant interfering peptide is presented here. This peptide of 29 aa strongly interacts with the nucleocapsid proteins (N) of different tospoviruses. Transgenic Nicotiana benthamiana lines expressing the peptide fused to a carrier protein were challenged with five different tospoviruses that have a nucleocapsid protein interacting with the peptide. In the transgenic plants, strong resistance to tomato spotted wilt virus, tomato chlorotic spot virus, groundnut ring spot virus, and chrysanthemum stem necrosis virus was observed. This therefore demonstrates the feasibility of using peptide ''aptamers'' as an in vivo tool to control viral infection in higher plants.

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Disruption of virus movement confers broad-spectrum resistance against systemic infection by plant viruses with a triple gene block.

Cited by 90 publications

References 39 publications

Conversion in the Requirement of Coat Protein in Cell-to-Cell Movement Mediated by the Cucumber Mosaic Virus Movement Protein

Conversion in the Requirement of Coat Protein in Cell-to-Cell Movement Mediated by the Cucumber Mosaic Virus Movement Protein

Estimating the agronomic impact of white clover mosaic virus on white clover performance in the North Island of New Zealand

Peptide-mediated broad-spectrum plant resistance to tospoviruses

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