Disruption of the Sugar Transporters AtSWEET11 and AtSWEET12 Affects Vascular Development and Freezing Tolerance in Arabidopsis

Hir, Rozenn Le; Spinner, Lara; Klemens, Patrick A.W.; Chakraborti, Dipankar; Marco, Federica De; Vilaine, Françoise; Wolff, Nelly; Lemoine, Rémi; Porcheron, Benoît; Géry, Carine; Téoulé, Evelyne; Chabout, Salem; Mouille, Grégory; Neuhaus, H. Ekkehard; Dinant, Sylvie; Bellini, Catherine

doi:10.1016/j.molp.2015.08.007

Cited by 123 publications

(127 citation statements)

References 10 publications

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“…Furthermore, the double Arabidopsis mutants sweet11-sweet12 exhibited greater freezing tolerance than the wild type and both single mutants by modifi cation of the chemical composition of the xylem (Le Hir et al 2015 ). These fi ndings suggested that AtSWEET11 and AtSWEET12 have a role as sugar exporters delivering carbon-containing skeletons to developing xylem cells to support secondary cell wall formation besides their role in contributing phloem loading in source leaves (Le Hir et al 2015 ). SWEET9 is involved in the secretion of nectar (Lin et al 2014 ) that is essential as food for pollinating insects (Sargent 2004 ).…”

Section: Sweet Proteinsmentioning

confidence: 99%

“…1 ). Le Hir et al ( 2015 ) very recently reported that AtSWEET11 and AtSWEET12 to transport glucose and fructose in addition to sucrose and their expression localized not only in phloem parenchyma cells but also in xylem cells close to the cambium region. Furthermore, the double Arabidopsis mutants sweet11-sweet12 exhibited greater freezing tolerance than the wild type and both single mutants by modifi cation of the chemical composition of the xylem (Le Hir et al 2015 ).…”

Section: Sweet Proteinsmentioning

confidence: 99%

“…Le Hir et al ( 2015 ) very recently reported that AtSWEET11 and AtSWEET12 to transport glucose and fructose in addition to sucrose and their expression localized not only in phloem parenchyma cells but also in xylem cells close to the cambium region. Furthermore, the double Arabidopsis mutants sweet11-sweet12 exhibited greater freezing tolerance than the wild type and both single mutants by modifi cation of the chemical composition of the xylem (Le Hir et al 2015 ). These fi ndings suggested that AtSWEET11 and AtSWEET12 have a role as sugar exporters delivering carbon-containing skeletons to developing xylem cells to support secondary cell wall formation besides their role in contributing phloem loading in source leaves (Le Hir et al 2015 ).…”

Section: Sweet Proteinsmentioning

confidence: 99%

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In Silico Functional Analyses of SWEETs Reveal Cues for Their Role in AMF Symbiosis

Sameeullah

Demiral

Aslam

et al. 2016

Plant, Soil and Microbes

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SWEETs are novel class of sugar effl uxers, which have unique functional role in plant biology. Besides nectar production, freezing tolerance, and transport of hexoses across tonoplast and growth-supporting role of pathogens, these SWEETs could have potential role in establishing powerful symbiotic relationship at the root interface and also in feeding to arbuscular mycorrhizal fungi (AMF) symbionts. The microarray or transcriptome expression of SWEET genes from colonized roots revealed that out of 28 Medicago SWEETs, three genes ( MtSWEET1b , MtSWEET3c , and MtSWEET12 ) were induced specifi cally due to AMF symbiosis. The root type specifi c expression of these three genes was also enhanced by AMF colonization in rice. The degree of expression of OsSWEET1b , OsSWEET3b , and OsSWEET12 was increased in colonized large lateral roots (LLRM) and crown roots (CRM), while OsSWEET3b and OsSWEET12 were induced in fi ne lateral roots (FLRM) and CRM, respectively. Promoter regions of these SWEETs represent critical motif elements (MYCS, PB1S, and PHR1) which play critical role in establishment of AMF symbiosis and phosphate starvation-induced responses, respectively. Taken together, these SWEETs have potential to be explored via functional genomics tools to understand feeding mechanisms to symbionts.

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Section: Sweet Proteinsmentioning

confidence: 99%

Section: Sweet Proteinsmentioning

confidence: 99%

Section: Sweet Proteinsmentioning

confidence: 99%

See 1 more Smart Citation

In Silico Functional Analyses of SWEETs Reveal Cues for Their Role in AMF Symbiosis

Sameeullah

Demiral

Aslam

et al. 2016

Plant, Soil and Microbes

View full text Add to dashboard Cite

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“…Furthermore, the recently identified sucrose efflux carriers, SWEETs, are components of the apoplasmic phloem loading in Arabidopsis leaves (Chen et al ). Interestingly, a sweet11sweet12 double mutant in Arabidopsis showed defects in the vascular development and xylem secondary cell wall formation of the inflorescence stem (Le Hir et al ). This study suggested that SWEET11 and 12 are involved in delivering carbon to developing xylem to support SCW biosynthesis.…”

Section: Sucrose Transport Into Woodmentioning

confidence: 99%

“…This study suggested that SWEET11 and 12 are involved in delivering carbon to developing xylem to support SCW biosynthesis. However, the Arabidopsis SWEET11 and SWEET12 are expressed in both phloem and the xylem tissues (Chen et al , Le Hir et al ), and it remains to be established whether the xylem phenotype is due to sucrose efflux defect in the phloem and/or in the xylem tissues. The recently published aspen transcriptome data supports a SWEET function in wood formation; six of the 23 orthologues of Arabidopsis SWEETs are expressed in the developing wood of aspen (Sundell et al ) (Fig.…”

Section: Sucrose Transport Into Woodmentioning

confidence: 99%

Sucrose transport and carbon fluxes during wood formation

Mahboubi

Niittylä

2018

Physiologia Plantarum

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Wood biosynthesis defines the chemical and structural properties of wood. The metabolic pathways that produce the precursors of wood cell wall polymers have a central role in defining wood properties. To make rational design of wood properties feasible, we need not only to understand the cell wall biosynthetic machinery, but also how sucrose transport and metabolism in developing wood connect to cell wall biosynthesis and how they respond to genetic and environmental cues. Here, we review the current understanding of the sucrose transport and primary metabolism pathways leading to the precursors of cell wall biosynthesis in woody plant tissues. We present both old, persistent questions and new emerging themes with a focus on wood formation in trees and draw upon evidence from the xylem tissues of herbaceous plants when it is relevant.

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AtSWEET11 and AtSWEET12 transporters function in tandem to modulate sugar flux in plants

et al. 2023

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The sugar will eventually be exported transporter (SWEET) members in Arabidopsis, AtSWEET11 and AtSWEET12 are the important sucrose efflux transporters that act synergistically to perform distinct physiological roles. These two transporters are involved in apoplasmic phloem loading, seed filling, and sugar level alteration at the site of pathogen infection. Here, we performed the structural analysis of the sucrose binding pocket of AtSWEET11 and AtSWEET12 using molecular docking followed by rigorous molecular dynamics (MD) simulations. We observed that the sucrose molecule binds inside the central cavity and in the middle of the transmembrane (TM) region of AtSWEET11 and AtSWEET12, that allows the alternate access to the sucrose molecule from either side of the membrane during transport. Both AtSWEET11 and AtSWEET12, shares the similar amino acid residues that interact with sucrose molecule. Further, to achieve more insights on the role of these two transporters in other plant species, we did the phylogenetic and the in-silico analyses of AtSWEET11 and AtSWEET12 orthologs from 39 economically important plants.We reported the extensive information on the gene structure, protein domain and cis-acting regulatory elements of AtSWEET11 and AtSWEET12 orthologs from different plants. The cis-elements analysis indicates the involvement of AtSWEET11 and AtSWEET12 orthologs in plant development and also during abiotic and biotic stresses. Both in silico and in planta expression analysis indicated AtSWEET11 and AtSWEET12 are well-expressed in the Arabidopsis leaf tissues. However, the orthologs of AtSWEET11 and AtSWEET12 showed the differential expression pattern with high or no transcript expression in the leaf tissues of different plants. Overall, these results offer the new insights into the functions and regulation of AtSWEET11 and AtSWEET12 orthologs from different plant species. This might be helpful in conducting the future studies to understand the role of these two crucial transporters in Arabidopsis and other crop plants.

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Disruption of the Sugar Transporters AtSWEET11 and AtSWEET12 Affects Vascular Development and Freezing Tolerance in Arabidopsis

Cited by 123 publications

References 10 publications

In Silico Functional Analyses of SWEETs Reveal Cues for Their Role in AMF Symbiosis

In Silico Functional Analyses of SWEETs Reveal Cues for Their Role in AMF Symbiosis

Sucrose transport and carbon fluxes during wood formation

AtSWEET11 and AtSWEET12 transporters function in tandem to modulate sugar flux in plants

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