Disruption of the PI3K/AKT/mTOR signaling cascade and induction of apoptosis in HL-60 cells by an essential oil from Monarda citriodora

Pathania, Anup Singh; Guru, Santosh Kumar; Verma, Mahendra K.; Sharma, Charu; Abdullah, Sheikh Tasduq; Malik, Fayaz; Chandra, Suresh; Katoch, Meenu; Bhushan, Shashi

doi:10.1016/j.fct.2013.08.037

Cited by 62 publications

(39 citation statements)

References 25 publications

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“…Four human cancer cell lines: HCT-116 (colorectal carcinoma), A-549 (lung), MCF-7 (breast), PC-3 (prostate) were procured from National Centre for Cell Sciences (NCCS), Pune, India for the present study. The MTT assay was performed as described by Pathania et al [24]. Cells were cultured in RPMI-1640 medium with 10% fetal calf serum (FCS), and 100U penicillin/100 μg mL −1 streptomycin.…”

Section: Bioactivity Evaluationmentioning

confidence: 99%

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Diversity, Phylogeny, anticancer and antimicrobial potential of fungal endophytes associated with Monarda citriodora L.

et al. 2017

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BackgroundPresent study focuses on diversity and distribution analysis of endophytic fungi associated with different tissues of the Monarda citriodora Cerv. ex Lag. (Lamiaceae/Labiatae). Anticancer and antimicrobial potential of isolated endophytes have also been investigated.ResultsA total of twenty eight fungal endophytes belonging to 11 different genera were isolated from this plant. All the endophytic fungi belonged to the Ascomycota phylum. The leaves were immensely rich in fungal species, while roots showed the highest tissue specific fungal dominance. Out of 28 fungal species, 72% endophytic extracts were found cytotoxic against one or more human cancer cell lines. The most prominent anticancer activity (IC50 value <10 μg/mL) was shown by MC-14 L (Fusarium oxysporum), MC-14 F (F. oxysporum), MC-18 L (Aspergillus fumigatus), MC-24 L (Cladosporium tenuissimum), MC-25 L (Fusarium sp.), MC-26 F (F. oxysporum) extracts. 75% of the extracts showed antimicrobial activities in agar disc-diffusion assay and 27% in the tube dilution method (MIC <100 μg/mL) respectively against the tested pathogens. Extracts of MC-14 L (F. oxysporum) and MC-18 L (A. fumigatus) displayed broad spectrum antimicrobial activity.ConclusionsThese results indicated that M. citriodora harbors a rich fungal endophytic community with anticancer and antimicrobial activities. The isolated endophyte MC-24 L (C. tenuissimum) has the potential to be a source of novel cytotoxic/antimicrobial compounds. This is the first report of diversity of fungal endophytes isolated from M. citriodora.Electronic supplementary materialThe online version of this article (doi:10.1186/s12866-017-0961-2) contains supplementary material, which is available to authorized users.

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Section: Bioactivity Evaluationmentioning

confidence: 99%

“…Major component of its essential oil, thymol, is now-a-days used in modern commercial mouthwash formulations [20, 22, 23]. Recently its essential oil is found to have anticancer property targeting PI3K pathway [24]. …”

Section: Introductionmentioning

confidence: 99%

Diversity, Phylogeny, anticancer and antimicrobial potential of fungal endophytes associated with Monarda citriodora L.

et al. 2017

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“…Previously, linalool has been reported to inhibit VEGF and TGF-β1 expression in different experimental models. [35][36][37] Linalool possessed the strongest inhibitory activity against various carcinoma cells. 18,38 Apoptosis is a controlled mechanism which limits the survival of cells with irreparable cellular damage and avoids malignant transformation.…”

Section: Discussionmentioning

confidence: 99%

The preventive effect of linalool on acute and chronic UVB-mediated skin carcinogenesis in Swiss albino mice

Gunaseelan

Balupillai

Kanimozhi

et al. 2016

Photochem Photobiol Sci

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In this study, we evaluated the role of linalool in acute ultraviolet-B (UVB; 280-320 nm) radiation-induced inflammation and chronic UVB-mediated photocarcinogenesis in mouse skin. Acute UVB-irradiation (180 mJ cm(-2)) causes hyperplasia, edema formation, lipid peroxidation, antioxidant depletion, and overexpression of cyclooxygenase-2 (COX-2) and ornithine decarboxylase (ODC) in mouse skin. Topical or intraperitoneal (i.p.) treatment of linalool prevented acute UVB-induced hyperplasia, edema formation, lipid peroxidation, and antioxidant depletion in mouse skin. Further, linalool treatment prevented UVB-induced overexpression of COX-2 and ODC in mouse skin. In the chronic study, mice were subjected to UVB-exposure thrice weekly for 30 weeks. Chronic UVB-exposure induced tumor incidence and expression of proliferative markers such as NF-κB, TNF-α, IL-6, COX-2, VEGF, TGF-β1, Bcl-2 and mutated p53 in mouse skin. Treatment with linalool before each UVB-exposure significantly prevented the expression of these proliferative markers and subsequently decreased the tumor incidence in mice skin. Histopathological studies confirmed the development of dysplasia and squamous cell carcinoma (SCC) in the chronic UVB-exposed mouse skin; and this was prevented by both topical and i.p. linalool treatment. Therefore, linalool may be considered as a photochemopreventive agent against UVB radiation induced skin carcinogenesis.

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“…Methodologies also revealed notably enhanced mitochondrial membrane permeability and increase expression of caspase-9. Intriguingly, PI3K/AKT/mTOR pathway was also inhibited significantly in treated HL-60 cells (Pathania et al, 2013). It is interesting to note that bioactive components in essential oil extracted from Dracocephalum multicaule protected K562 cells from oxidative stress (Esmaeili et al, 2014).…”

Section: In-vitro Analysis Of Different Essential Oilsmentioning

confidence: 99%

Anticancer Activity of Essential Oils: Targeting of Protein Networks in Cancer Cells

Aras

Iqbal²,

Naqvi³

et al. 2014

Asian Pacific Journal of Cancer Prevention

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Disruption of the PI3K/AKT/mTOR signaling cascade and induction of apoptosis in HL-60 cells by an essential oil from Monarda citriodora

Cited by 62 publications

References 25 publications

Diversity, Phylogeny, anticancer and antimicrobial potential of fungal endophytes associated with Monarda citriodora L.

Diversity, Phylogeny, anticancer and antimicrobial potential of fungal endophytes associated with Monarda citriodora L.

The preventive effect of linalool on acute and chronic UVB-mediated skin carcinogenesis in Swiss albino mice

Anticancer Activity of Essential Oils: Targeting of Protein Networks in Cancer Cells

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