Disruption of the interaction between TFIIAαβ and TFIIA recognition element inhibits RNA polymerase II gene transcription in a promoter context-dependent manner

Wang, Juan; Shi, Kaituo; Wu, Zihui; Zhang, Cheng; Li, Yuan; Deng, Huan; Zhao, Shasha; Deng, Wensheng

doi:10.1016/j.bbagrm.2020.194611

Cited by 3 publications

(2 citation statements)

References 37 publications

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“…Pdp1 (HLF) is a widely expressed transcription factor critical for proper development and circadian rhythm(35,36). TfIIA-L (GTF2A1) is a member of the preinitiation complex required for RNA polymerase II initiation(37). The precise mechanism of how Pdp1 (HLF) and TfIIA-L (GTF2A1) impact preconditioning is unclear, but their known roles suggest the possible importance of transcriptional regulation in preconditioning.…”

Section: Resultsmentioning

confidence: 99%

ADrosophilascreen identifies a role for histone methylation in ER stress preconditioning

Owings

Chow

2023

Preprint

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Stress preconditioning occurs when transient, sublethal stress events impact an organism's ability to counter future stresses. Although preconditioning effects are often noted in the literature, very little is known about the underlying mechanisms. To model preconditioning, we exposed a panel of genetically diverse Drosophila melanogaster to a sublethal heat shock and measured how well the flies survived subsequent exposure to endoplasmic reticulum (ER) stress. The impact of preconditioning varied with genetic background, ranging from dying half as fast to four and a half times faster with preconditioning compared to no preconditioning. Subsequent association and transcriptional analyses revealed that histone methylation, transcriptional regulation, and immune status are all candidate preconditioning modifier pathways. Strikingly, almost all subunits (7/8) in the Set1/COMPASS complex were identified as candidate modifiers of preconditioning. Functional analysis of Set1 knockdown flies demonstrated that loss of Set1 led to the transcriptional dysregulation of canonical ER stress genes during preconditioning. Based on these analyses, we propose a model of preconditioning in which Set1 helps to establish an interim transcriptional 'memory' of previous stress events, resulting in a preconditioned response to subsequent stress.

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Section: Resultsmentioning

confidence: 99%

ADrosophilascreen identifies a role for histone methylation in ER stress preconditioning

Owings

Chow

2023

Preprint

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“…A second general transcription factor was also identified, TfIIA-L ( GTF2A1 ). TfIIA-L ( GTF2A1 ) is a member of the preinitiation complex required for RNA polymerase II initiation ( Wang et al 2020 ). The precise mechanism of how Pdp1 ( HLF ) and TfIIA-L ( GTF2A1 ) impact preconditioning is unclear, but their known roles suggest the possible importance of transcriptional regulation in preconditioning.…”

Section: Resultsmentioning

confidence: 99%

A Drosophila screen identifies a role for histone methylation in ER stress preconditioning

Owings,

Chow

2023

G3: Genes, Genomes, Genetics

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Stress preconditioning occurs when transient, sublethal stress events impact an organism's ability to counter future stresses. Although preconditioning effects are often noted in the literature, very little is known about the underlying mechanisms. To model preconditioning, we exposed a panel of genetically diverse Drosophila melanogaster to a sublethal heat shock and measured how well the flies survived subsequent exposure to endoplasmic reticulum (ER) stress. The impact of preconditioning varied with genetic background, ranging from dying half as fast to 4 and a half times faster with preconditioning compared to no preconditioning. Subsequent association and transcriptional analyses revealed that histone methylation, and transcriptional regulation are both candidate preconditioning modifier pathways. Strikingly, almost all subunits (7/8) in the Set1/COMPASS complex were identified as candidate modifiers of preconditioning. Functional analysis of Set1 knockdown flies demonstrated that loss of Set1 led to the transcriptional dysregulation of canonical ER stress genes during preconditioning. Based on these analyses, we propose a preconditioning model in which Set1 helps to establish an interim transcriptional “memory” of previous stress events, resulting in a preconditioned response to subsequent stress.

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The Core Promoter Is a Regulatory Hub for Developmental Gene Expression

Sloutskin

Shir-Shapira

Freiman

et al. 2021

Front. Cell Dev. Biol.

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The development of multicellular organisms and the uniqueness of each cell are achieved by distinct transcriptional programs. Multiple processes that regulate gene expression converge at the core promoter region, an 80 bp region that directs accurate transcription initiation by RNA polymerase II (Pol II). In recent years, it has become apparent that the core promoter region is not a passive DNA component, but rather an active regulatory module of transcriptional programs. Distinct core promoter compositions were demonstrated to result in different transcriptional outputs. In this mini-review, we focus on the role of the core promoter, particularly its downstream region, as the regulatory hub for developmental genes. The downstream core promoter element (DPE) was implicated in the control of evolutionarily conserved developmental gene regulatory networks (GRNs) governing body plan in both the anterior-posterior and dorsal-ventral axes. Notably, the composition of the basal transcription machinery is not universal, but rather promoter-dependent, highlighting the importance of specialized transcription complexes and their core promoter target sequences as key hubs that drive embryonic development, differentiation and morphogenesis across metazoan species. The extent of transcriptional activation by a specific enhancer is dependent on its compatibility with the relevant core promoter. The core promoter content also regulates transcription burst size. Overall, while for many years it was thought that the specificity of gene expression is primarily determined by enhancers, it is now clear that the core promoter region comprises an important regulatory module in the intricate networks of developmental gene expression.

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Disruption of the interaction between TFIIAαβ and TFIIA recognition element inhibits RNA polymerase II gene transcription in a promoter context-dependent manner

Cited by 3 publications

References 37 publications

ADrosophilascreen identifies a role for histone methylation in ER stress preconditioning

ADrosophilascreen identifies a role for histone methylation in ER stress preconditioning

A Drosophila screen identifies a role for histone methylation in ER stress preconditioning

The Core Promoter Is a Regulatory Hub for Developmental Gene Expression

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