Disruption of the copper efflux pump (CopA) of Serratia marcescens ATCC 274 pleiotropically affects copper sensitivity and production of the tripyrrole secondary metabolite, prodigiosin

Williamson, Neil R.; Simonsen, Henrik Toft; Harris, Abigail K. P.; Leeper, Finian J.; Salmond, George P. C.

doi:10.1007/s10295-005-0040-9

Cited by 25 publications

(15 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…One exception was Serratia marcescens, in which cueR and copA are separated by a cluster of genes encoding for the biosynthesis of the antibiotic prodigiosin (pigA-O). Expression of pig genes is repressed by Cu but the physiological relevance of this observation is unclear (11). In agreement with a previous report, ybaS is also present in nine additional genera from the Enterobacteriacae family, including Edwardsiella and Yersinia.…”

Section: Resultssupporting

confidence: 90%

Interplay between tolerance mechanisms to copper and acid stress inEscherichia coli

Djoko

Phan

Peters

et al. 2017

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

Significance Copper (Cu) is an essential trace metal nutrient in health and is increasingly recognized for its role in the control of infection. The pathogen Escherichia coli encounters host niches with mild to high acidity and elevated copper levels. Our study shows that this bacterium can alter its metabolism and harness the amino acid glutamine to suppress the effects of acid stress and copper toxicity. Given the abundance of glutamine in systemic circulation and its importance in the host immune system, our work provides a new insight into the ways in which bacterial pathogens can adapt and survive host-imposed antibacterial strategies.

show abstract

Section: Resultssupporting

confidence: 90%

Interplay between tolerance mechanisms to copper and acid stress inEscherichia coli

Djoko

Phan

Peters

et al. 2017

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

show abstract

“…The prodigiosin biosynthetic gene cluster family member Pigb encodes phosphatidylinositol glycan class B. This protein has a variety of functions in a number of species [56], [57] from conferring mercury and copper resistance [58], [59] to facilitating signaling in cells [60], [61]. Therefore, the epigenetic alterations observed in vinclozolin lineage E13 PGCs were distinct from those in E16 prospermatogonia.…”

Section: Discussionmentioning

confidence: 99%

Environmentally Induced Transgenerational Epigenetic Reprogramming of Primordial Germ Cells and the Subsequent Germ Line

et al. 2013

View full text Add to dashboard Cite

A number of environmental factors (e.g. toxicants) have been shown to promote the epigenetic transgenerational inheritance of disease and phenotypic variation. Transgenerational inheritance requires the germline transmission of altered epigenetic information between generations in the absence of direct environmental exposures. The primary periods for epigenetic programming of the germ line are those associated with primordial germ cell development and subsequent fetal germline development. The current study examined the actions of an agricultural fungicide vinclozolin on gestating female (F0 generation) progeny in regards to the primordial germ cell (PGC) epigenetic reprogramming of the F3 generation (i.e. great-grandchildren). The F3 generation germline transcriptome and epigenome (DNA methylation) were altered transgenerationally. Interestingly, disruptions in DNA methylation patterns and altered transcriptomes were distinct between germ cells at the onset of gonadal sex determination at embryonic day 13 (E13) and after cord formation in the testis at embryonic day 16 (E16). A larger number of DNA methylation abnormalities (epimutations) and transcriptional alterations were observed in the E13 germ cells than in the E16 germ cells. These observations indicate that altered transgenerational epigenetic reprogramming and function of the male germline is a component of vinclozolin induced epigenetic transgenerational inheritance of disease. Insights into the molecular control of germline transmitted epigenetic inheritance are provided.

show abstract

“…A copA mutant, with a chloramphenicol resistance (Cm R ) cassette inserted into the copA gene, was constructed in Sma 274 and showed normal prodigiosin production in unsupplemented LB medium (Williamson et al, 2006). Since copA is adjacent to the pig cluster, this mutation provided a selectable marker (Cm R ) closely linked with the pig genes.…”

Section: Resultsmentioning

confidence: 99%

“…A random transposon mutagenesis of Sma 12 was performed. E. coli S17-1 lpir carrying pUTminiTn5Km1 was used to introduce the transposon into Sma 12 by conjugation as described previously (Williamson et al, 2006). A total of 2700 Km R transconjugants were picked onto lawns of the aHSL bioassay strain, Chromobacterium violaceum CV026.…”

mentioning

confidence: 99%

Metabolic and regulatory engineering of Serratia marcescens: mimicking phage-mediated horizontal acquisition of antibiotic biosynthesis and quorum-sensing capacities

et al. 2006

Self Cite

View full text Add to dashboard Cite

Serratia marcescens is an important cause of opportunistic human infections. Many, but not all, strains produce prodigiosin, a secondary metabolic, red-pigment antibiotic, the biosynthesis of which is directed by the pig gene cluster. Quorum sensing (QS) involves the production and detection of chemical signal molecules as a means to regulate gene expression in response to population cell density. Several strains of S. marcescens have previously been shown to possess an N-acyl-L-homoserine lactone (aHSL) QS system. This study aimed to determine the impact of introducing, by phage-mediated horizontal gene transfer, a biosynthetic gene cluster (pig) and a regulatory locus (aHSL QS) into strains lacking the respective trait. The pig cluster from S. marcescens ATCC 274 (Sma 274) was transferred to the non-pigmented strain, S. marcescens strain 12 (Sma 12). In the engineered strain, pigment was expressed and brought under the control of the recipient's native regulatory systems (aHSL QS and luxS). Moreover, transfer of the aHSL locus from Sma 12 to the non-QS Sma 274 resulted in the imposition of aHSL control onto a variety of native traits, including pigment production. In addition, during this study, the QS regulon of the clinical strain, Sma 12, was characterized, and some novel QS-regulated traits in S. marcescens were identified. The results have implications for the evolution and dissemination of biosynthetic and QS loci, illustrating the genetic modularity and ease of acquisition of these traits and the capacity of phages to act as vectors for horizontal gene transfer. INTRODUCTIONSerratia marcescens is a Gram-negative, enteric bacterium that is able to inhabit a wide variety of ecological niches and cause disease in plant, vertebrate and invertebrate hosts (Grimont & Grimont, 1978). It is an opportunistic human pathogen and is responsible for an increasing number of serious nosocomial infections, a problem exacerbated by the resistance of many strains to multiple antibiotics (Hejazi & Falkiner, 1997;Aucken & Pitt, 1998). S. marcescens strains produce a range of secreted products, including proteases, nuclease, lipase, chitinases and haemolysin, many of which are likely to represent virulence factors in human infection (Aucken & Pitt, 1998;Braun et al., 1993;Hines et al., 1988;Hejazi & Falkiner, 1997). A characteristic feature of many S. marcescens strains, particularly those of environmental origin, is production of the red tripyrrole antibiotic prodigiosin (2-methyl-3-pentyl-6-methoxyprodigiosin). Prodigiosins are currently of great interest because they have been shown to possess antimicrobial, antiprotozoal, immunosuppressive and anti-oncogenic properties (Williamson et al., 2005 and references therein). However, the physiological role of prodigiosin in the producing organism remains elusive. In this study we used two strains of S. marcescens: S. marcescens ATCC 274 (Sma 274) is a pigmented, environmental strain, whereas S. marcescens strain 12 (Sma 12) is a non-pigmented, clinical isolate.Previously, we h...

show abstract

Disruption of the copper efflux pump (CopA) of Serratia marcescens ATCC 274 pleiotropically affects copper sensitivity and production of the tripyrrole secondary metabolite, prodigiosin

Cited by 25 publications

References 32 publications

Interplay between tolerance mechanisms to copper and acid stress inEscherichia coli

Interplay between tolerance mechanisms to copper and acid stress inEscherichia coli

Environmentally Induced Transgenerational Epigenetic Reprogramming of Primordial Germ Cells and the Subsequent Germ Line

Metabolic and regulatory engineering of Serratia marcescens: mimicking phage-mediated horizontal acquisition of antibiotic biosynthesis and quorum-sensing capacities

Contact Info

Product

Resources

About