Disruption of putative short-chain acyl-CoA dehydrogenases compromised free radical scavenging, conidiogenesis, and pathogenesis of Magnaporthe oryzae

Aliyu, Sami Rukaiya; Lin, Lili; Chen, Xiaomin; Waheed, Abdul; Lin, Yunzhi; Otieno, Frankine Jagero; Shabbir, Abdul; Batool, Wajjiha; Zhang, Yiqun; Tang, Wei; Wang, Zonghua; Norvienyeku, Justice

doi:10.1016/j.fgb.2019.02.010

Cited by 18 publications

(32 citation statements)

References 46 publications

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“…To evaluate the influence of MoeIF4E3 on the infectious development of the rice blast fungus during host-pathogen interaction, hyphae and spores obtained from Δ Moeif4e3 , wild-type, and the complementation strain, were used to inoculate 8-days-old barley leaves as previously described ( Li X. et al, 2017 ; Aliyu et al, 2019 ). Assessment of infection characteristics of the individual strains at 7-days post-inoculation (dpi) showed that the inactivation of MoeIF4E3 severely compromised the penetration and virulence of the Δ Moeif4e3 strains on intact and injured barley leaves ( Figure 5A ).…”

Section: Resultsmentioning

confidence: 99%

“…For it, 0.8 kb upstream and 0.9 kb downstream regions were amplified using MoeIFAF/AR and MoeIFBF/BR primers pairs, respectively ( Supplementary Table 3 ). The resulting PCR products were ligated with a hph cassette fragment amplified with primers HYG/F+HY/R and YG/R+HYG/R ( Aliyu et al, 2019 ) by overlapping PCR. For transformation, targeted gene deletion using a homologous recombination approach was used as described ( Zhang et al, 2016 ).…”

Section: Methodsmentioning

confidence: 99%

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Translation Initiation Factor eIF4E Positively Modulates Conidiogenesis, Appressorium Formation, Host Invasion and Stress Homeostasis in the Filamentous Fungi Magnaporthe oryzae

et al. 2021

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Translation initiation factor eIF4E generally mediates the recognition of the 5’cap structure of mRNA during the recruitment of the ribosomes to capped mRNA. Although the eIF4E has been shown to regulate stress response in Schizosaccharomyces pombe positively, there is no direct experimental evidence for the contributions of eIF4E to both physiological and pathogenic development of filamentous fungi. We generated Magnaporthe oryzae eIF4E (MoeIF4E3) gene deletion strains using homologous recombination strategies. Phenotypic and biochemical analyses of MoeIF4E3 defective strains showed that the deletion of MoeIF4E3 triggered a significant reduction in growth and conidiogenesis. We also showed that disruption of MoeIF4E3 partially impaired conidia germination, appressorium integrity and attenuated the pathogenicity of ΔMoeif4e3 strains. In summary, this study provides experimental insights into the contributions of the eIF4E3 to the development of filamentous fungi. Additionally, these observations underscored the need for a comprehensive evaluation of the translational regulatory machinery in phytopathogenic fungi during pathogen-host interaction progression.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Translation Initiation Factor eIF4E Positively Modulates Conidiogenesis, Appressorium Formation, Host Invasion and Stress Homeostasis in the Filamentous Fungi Magnaporthe oryzae

et al. 2021

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“…The availability of mitochondrial genome sequences provides valuable information about genome organization and enables evaluating structural rearrangements using comparative studies [27]. The high rate of polymorphism frequently found within introns or intergenic regions of well-conserved mitochondrial genes makes these sequences useful for genetic diversity studies, both among and within populations [28][29][30][31].…”

Section: Introductionmentioning

confidence: 99%

“…oxidation plays an essential role in vegetative growth, conidiation, appressorial morphogenesis, and pathogenesis progression in M. oryzae [31]. The methylation of the mitochondrial genome is an epigenetic mechanism affecting the adaptation and pathogenicity of Candida albicans [32].…”

Section: Introductionmentioning

confidence: 99%

Revealing the high variability on nonconserved core and mobile elements of Austropuccinia psidii and other rust mitochondrial genomes

et al. 2021

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Mitochondrial genomes are highly conserved in many fungal groups, and they can help characterize the phylogenetic relationships and evolutionary biology of plant pathogenic fungi. Rust fungi are among the most devastating diseases for economically important crops around the world. Here, we report the complete sequence and annotation of the mitochondrial genome of Austropuccinia psidii (syn. Puccinia psidii), the causal agent of myrtle rust. We performed a phylogenomic analysis including the complete mitochondrial sequences from other rust fungi. The genome composed of 93.299 bp has 73 predicted genes, 33 of which encoded nonconserved proteins (ncORFs), representing almost 45% of all predicted genes. A. psidii mtDNA is one of the largest rust mtDNA sequenced to date, most likely due to the abundance of ncORFs. Among them, 33% were within intronic regions of diverse intron groups. Mobile genetic elements invading intron sequences may have played significant roles in size but not shaping of the rust mitochondrial genome structure. The mtDNAs from rust fungi are highly syntenic. Phylogenetic inferences with 14 concatenated mitochondrial proteins encoded by the core genes placed A. psidii according to phylogenetic analysis based on 18S rDNA. Interestingly, cox1, the gene with the greatest number of introns, provided phylogenies not congruent with the core set. For the first time, we identified the proteins encoded by three A. psidii ncORFs using proteomics analyses. Also, the orf208 encoded a transmembrane protein repressed during in vitro morphogenesis. To the best of our knowledge, we presented the first report of a complete mtDNA sequence of a member of the family Sphaerophragmiacea.

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“…The major biotic factors affecting rice farmers in Malaysia are bacterial leaf blight, rice blast and sheath blight. The rice blast disease of cultivated rice is a problem compounded by climate change and modern agricultural practices that makes this disease uncontrollable, resulting in major yield losses worldwide [ 2 , 3 , 4 ]. This disease threatens global food security, where it is estimated that enough rice to feed 60 million people is destroyed by rice blast disease yearly [ 5 , 6 , 7 , 8 ].…”

Section: Introductionmentioning

confidence: 99%

Fatty Acid Synthase Beta Dehydratase in the Lipid Biosynthesis Pathway Is Required for Conidiogenesis, Pigmentation and Appressorium Formation in Magnaporthe oryzae S6

Sangappillai

Nadarajah

2020

IJMS

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Lipid biosynthesis produces glycerol, which is important in fueling turgor pressure necessary for germination and penetration of plant host by fungi. As the relationship between pathogenicity and the lipid biosynthetic pathway is not fully understood, we have elucidated the role of the fatty acid synthase beta subunit dehydratase (FAS1) gene in lipid biosynthesis. The FAS1 gene was silenced through homologous double crossover in Magnaporthe oryzae strain S6 to study the effect on lipid biosynthesis. The vegetative growth of Δfas1 mutants show the highest drop on oleic acid (between 10 and 50%), while the mycelial dry weight of mutants dropped significantly on all media. Conidiation of FAS1 mutants show a ~10- and ~5-fold reduction on oatmeal and Potato Dextrose Agar (PDA), respectively. Mutants formed mycelium that were mildly pigmented, indicating that the deletion of FAS1 may have affected melanin biosynthesis. Biochemical and gene expression studies concluded that the fatty acid degradation pathway might have been interrupted by FAS1 deletion. FAS1 mutants showed no enzyme activity on glucose or olive oil, suggesting that the mutants may lack functional peroxisomes and be defective in β-oxidation of fatty acids, hence explaining the reduced lipid deposits in the spores.

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Disruption of putative short-chain acyl-CoA dehydrogenases compromised free radical scavenging, conidiogenesis, and pathogenesis of Magnaporthe oryzae

Cited by 18 publications

References 46 publications

Translation Initiation Factor eIF4E Positively Modulates Conidiogenesis, Appressorium Formation, Host Invasion and Stress Homeostasis in the Filamentous Fungi Magnaporthe oryzae

Translation Initiation Factor eIF4E Positively Modulates Conidiogenesis, Appressorium Formation, Host Invasion and Stress Homeostasis in the Filamentous Fungi Magnaporthe oryzae

Revealing the high variability on nonconserved core and mobile elements of Austropuccinia psidii and other rust mitochondrial genomes

Fatty Acid Synthase Beta Dehydratase in the Lipid Biosynthesis Pathway Is Required for Conidiogenesis, Pigmentation and Appressorium Formation in Magnaporthe oryzae S6

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