Disruption of HPV16-E7 by CRISPR/Cas System Induces Apoptosis and Growth Inhibition in HPV16 Positive Human Cervical Cancer Cells

Hu, Zheng; Yu, Lan; Zhu, Da; Ding, Wencheng; Wang, Xiaoli; Zhang, Changlin; Wang, Liming; Jiang, Xiaohui; Shen, Hui; He, Dan; Li, Kezhen; Liu, Xi; Wang, Hui

doi:10.1155/2014/612823

Cited by 162 publications

(132 citation statements)

References 19 publications

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“…The therapeutic mechanism of CRISPR/Cas9-mediated silencing of both E6 and E7 mainly depends on the reactivation of TP53 and pRB to induce apoptosis and cellular senescence. Yu et al [74] have reported a CRISPR/Cas9 sequence targeting the E6 mRNA reduces the level of full-length E6 mRNA and increases the level of TP53 protein. Hu et al [75] also suggested that CRISPR/Cas9 inhibition of E7 as a potential therapeutic intervention for the treatment of cervical cancer.…”

Section: Therapeutics Against Hpvsmentioning

confidence: 99%

Oncogenic Human Papillomavirus: Application of CRISPR/Cas9 Therapeutic Strategies for Cervical Cancer

Zhen

2017

Cell Physiol Biochem

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Oncogenic human papillomaviruses (HPVs) cause different types of cancer especially cervical cancer. HPV-associated carcinogenesis provides a classical model system for clustered regularly interspaced short palindromic repeats (CRISPR/Cas9) based cancer therapies since the viral oncogenes E6 and E7 are exclusively expressed in cancerous cells. Sequence-specific gene knockdown/knockout using CRISPR/Cas9 shows promise as a novel therapeutic approach for the treatment of a variety of diseases that currently lack effective treatments. However, CRISPR/Cas9-based targeting therapy requires further validation of its efficacy in vitro and in vivo to eliminate the potential off-target effects, necessitates verification of the delivery vehicles and the combinatory use of conventional therapies with CRISPR/Cas9 to ensure the feasibility and safety. In this review we discuss the potential of combining CRISPR/Cas9 with other treatment options as therapies for oncogenic HPVs-associated carcinogenesis. and present our assessment of the promising path to the development of CRISPR/Cas9 therapeutic strategies for clinical settings.

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Section: Therapeutics Against Hpvsmentioning

confidence: 99%

Oncogenic Human Papillomavirus: Application of CRISPR/Cas9 Therapeutic Strategies for Cervical Cancer

Zhen

2017

Cell Physiol Biochem

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“…ZFNs, TALENs and CRISPR/Cas9 [64][65][66][67][68][69]. In 2014, Hu et al [70] reported that CRISPR-Cas9 mediated disruption of viral gene expression and replication in various cell lines suggest that HIV could be cure by using CRISPR/Cas9 editing tool.…”

Section: Other Genetic Diseasesmentioning

confidence: 99%

Application of CRISPR/Cas9 Genome Editing in Genetic Disorders: A Systematic Review Up to Date

Pandey¹,

Tripathi²,

Bhushan³

et al. 2017

J Genet Syndr Gene Ther

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Genetic diseases in human are associated with congenital disorders and phenotypic traits. A single mutation in a gene can cause physical or mental problems, and sometimes both. Some diseases can be lethal, and there are still no cures for many of them. Socioeconomic burden of rare genetic diseases are increasing worldwide that have been tried to cure using various methods. However, they were not very successful till now. Genome editing technologies over the past few years is providing fast and effective tool to precisely manipulate the genome at specific locations. Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) associated Cas9 (CRISPR/Cas9) system has been using from last few years in the field of biomedical research. CRISPR/Cas9 has advantages in terms of clinical applicability to treat genetic diseases like DMD, Hemophilia, β-Thalassemia and cystic fibrosis etc. and even in some cases this tool has already been successfully applied. Nevertheless, developed technologies for addition or deletion of genes have made notable progress in last few years and demonstrate some promising clinical results. However, several challenges still remain. Here, the latest applications of CRISPR-Cas9 technology in genetic disorders, current challenges and future directions are reviewed and discussed.

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“…Progressive genetic and epigenetic alterations will eventually develop cancer in a living organism and the CRISPR-Cas9 system has variable components with applications in treating and modelling this illness. Currently, there are trials focused on modelling oncogenic mutation in cell lines [29], adult animals [30] disabling oncogenic viruses [31] or manipulating the cancer genome [32].…”

Section: Medicinementioning

confidence: 99%

Review. Development, Applications, Benefits, Challenges and Limitations of the New Genome Engineering Technique. An Update Study

Crauciuc

Tripon

Gheorghiu

et al. 2017

Acta Medica Marisiensis

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We assume that the CRISPR Cas9 theory must be delimited by applicability, because the consequences of long term DNA manipulation remain unknown. Moreover, the irreversibility of this procedure should instigate researchers to reserved opinions. Usefulness as well as benefits of CRISPR Cas9 made it one of the most popular and used genome editing technique. But with its huge potential, ethical and safety concerns emerge. Therefore, before continuing research in this direction we should have a well organized system that is able to make that differentiation between research and reproduction. However we truly believe in the future of genetic engineering and with the CRISPR-Cas9 system we expect that the opportunity of treating now so called incurable diseases arises. Time is all we need.

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Disruption of HPV16-E7 by CRISPR/Cas System Induces Apoptosis and Growth Inhibition in HPV16 Positive Human Cervical Cancer Cells

Cited by 162 publications

References 19 publications

Oncogenic Human Papillomavirus: Application of CRISPR/Cas9 Therapeutic Strategies for Cervical Cancer

Oncogenic Human Papillomavirus: Application of CRISPR/Cas9 Therapeutic Strategies for Cervical Cancer

Application of CRISPR/Cas9 Genome Editing in Genetic Disorders: A Systematic Review Up to Date

Review. Development, Applications, Benefits, Challenges and Limitations of the New Genome Engineering Technique. An Update Study

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