Disruption of Chlorella vulgaris Cells for the Release of Biodiesel-Producing Lipids: A Comparison of Grinding, Ultrasonication, Bead Milling, Enzymatic Lysis, and Microwaves

Zheng, Hongli; Yin, Jinhua; Gao, Zhen; Huang, He; Ji, Xiao‐Jun; Dou, Chang

doi:10.1007/s12010-011-9207-1

Cited by 303 publications

(145 citation statements)

References 22 publications

(22 reference statements)

Supporting

Mentioning

132

Contrasting

Unclassified

Order By: Relevance

“…For example, bead milling is a common way to grind materials into fine powder by shear force, which is caused by the mechanical force. Zheng et al (2011) used bead milling as a pretreatment for microalgae, obtaining an approximately 10% lipid yield. However, a greater lipid yield could be obtained with manual grinding in liquid nitrogen.…”

Section: Mechanical Methodsmentioning

confidence: 99%

A Review: Microalgae and Their Applications in CO2 Capture and Renewable Energy

Klinthong

Yang

Huang

et al. 2015

Aerosol Air Qual. Res.

201

View full text Add to dashboard Cite

Fossil fuels, which are recognized as unsustainable sources of energy, are continuously consumed and decreased with increasing fuel demands. Microalgae have great potential as renewable fuel sources because they possess rapid growth rate and the ability to store high-quality lipids and carbohydrates inside their cells for biofuel production. Microalgae can be cultivated on opened or closed systems and require nutrients and CO 2 that may be supplied from wastewater and fossil fuel combustion. In addition, CO 2 capture via photosynthesis to directly fix carbon into microalgae has also attracted the attention of researchers. The conversion of CO 2 into chemical and fuel (energy) products without pollution via this approach is a promising way to not only reduce CO 2 emissions but also generate more economic value. The harvested microalgal biomass can be converted into biofuel products, such as biohydrogen, biodiesel, biomethanol, bioethanol, biobutanol and biohydrocarbons. Thus, microalgal cultivation can contribute to CO 2 fixation and can be a source of biofuels. This article reviews the literature on microalgae that were cultivated using captured CO 2 , technologies related to the production of biofuels from microalgae and the possible commercialization of microalgae-based biofuels to demonstrate the potential of microalgae. In this respect, a number of relevant topics are addressed: the nature of microalgae (e.g., species and composition); CO 2 capture via microalgae; the techniques for microalgal cultivation, harvesting and pretreatment; and the techniques for lipid extraction and biofuel production. The strategies for biofuel commercialization are proposed as well.

show abstract

Section: Mechanical Methodsmentioning

confidence: 99%

A Review: Microalgae and Their Applications in CO2 Capture and Renewable Energy

Klinthong

Yang

Huang

et al. 2015

Aerosol Air Qual. Res.

201

View full text Add to dashboard Cite

show abstract

“…The second method includes addition of Quartz sand to the sample and grounded directly. The third method includes drying the sample at 60°C for 7-8 h and grounded with Quartz sand [9].…”

Section: Manual Grindingmentioning

confidence: 99%

“…Treatment of C. vulgaris with lysozyme and cellulase resulted in lipid concentration of 22% and 24%, respectively, and had the highest efficiency among all enzymatic treatment (Zheng et al) [9]. Algal cell wall is made of many complex layers and lysis of all these layers with just cellulase and lipase is not possible.…”

Section: Cellulose Cellulase Glucosementioning

confidence: 99%

“…Its composition differs from each other, and hence, different disruption methods need to be developed. For example, the cell wall of Chlorella vulgaris predominantly consists of saccharides and hemicellulose for which grinding using liquid nitrogen has shown significant results [9]. The choice of the cell disruption technique depends on the type of algae, its cell wall structure, and composition.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

An Insight on Algal Cell Disruption for Biodiesel Production

Aarthy

Smita

Turkar

et al. 2018

Asian J Pharm Clin Res

View full text Add to dashboard Cite

Objective: This review article deals with the effect that various cell disruption techniques have on the efficiency of lipid extraction. We have reviewed existing algal cell disruption techniques that aid the biodiesel production process.Methods: Current rise in demand for energy has led the researcher to focus on the production of sustainable fuels, among which biodiesel has received greater attention. This is due to its larger lipid content, higher growth rate, larger biomass production, and lower land use. Extraction of lipid from algae (micro and macro) for the production of biodiesel involves numerous downstream processing steps, of which cell wall disruption is a crucial step. Bead milling, high-pressure homogenization, ultra-sonication, freeze-drying, acid treatment, and enzymatic lysis are some methods of cell disruption. The cell disruption technique needs to be optimized based on the structure and biochemical composition of algae. Result:The lipid extraction efficiency varies depending on the algal species and the cell disruption technique used. Conclusion:In-depth research and development of new techniques are required to further enhance the cell disruption of the algal cell wall for the enhanced recovery of lipids. In addition, the operating costs and energy consumption should also be optimized for the cost-effective recovery.

show abstract

“…The ultrasonication extraction process has been proven to efficiently destroy the hard cell walls of natural resources with high efficiency [13,14]. Moreover, ultrasound extraction could be an excellent alternative for avoiding high temperature during the processes, especially for extracting heat labile substances from natural resources, and it is also considered to be an excellent green process.…”

Section: Introductionmentioning

confidence: 99%

Enhancement of Skin Anti-Inflammatory Activities of Eclipta prostrata L. from the Ultrasonic Extraction Process

Lee

2017

Applied Sciences

View full text Add to dashboard Cite

This work first showed that the skin anti-inflammatory activities of Eclipta prostrata L. (E. prostrata L.)n be increased through an ultrasonic extraction process at a frequency of 120 kHz with 70% ethanol at 40 • C for 6 h. The extract from the ultrasound extraction (UE) contained 378.6 mg/100 g of wedelolactone (a major bioactive substance in E. prostrata L.), compared with the 172.9 mg/100 g from a conventional extraction process with 70% ethanol at 80 • C for 12 h (EE). The UE showed less than 10% of low cytotoxicity against normal fibroblast cells, and also exhibited much higher antioxidant activities than that from the EE when treating with 0.3 mg/mL of the extracts. It was proved that the high antioxidant activities of the UE were closely correlated with the enhancement of skin anti-inflammatory activities by effectively suppressing the production of tumor necrosis factor-alpha and interleukin-6 from macrophages. The production of prostaglandin E 2 from human skin cells was also greatly reduced, compared with the EE for all ranges of the treatments. These results strongly indicate that the various biological activities of E. prostrata L. can be improved by a simple ultrasound extraction process because of the high elution of bioactive substances even at a low temperature.

show abstract

Disruption of Chlorella vulgaris Cells for the Release of Biodiesel-Producing Lipids: A Comparison of Grinding, Ultrasonication, Bead Milling, Enzymatic Lysis, and Microwaves

Cited by 303 publications

References 22 publications

A Review: Microalgae and Their Applications in CO2 Capture and Renewable Energy

A Review: Microalgae and Their Applications in CO2 Capture and Renewable Energy

An Insight on Algal Cell Disruption for Biodiesel Production

Enhancement of Skin Anti-Inflammatory Activities of Eclipta prostrata L. from the Ultrasonic Extraction Process

Contact Info

Product

Resources

About