Disrupting MALAT1/miR-200c sponge decreases invasion and migration in endometrioid endometrial carcinoma

Li, Qiulian; Zhang, Chao; Chen, Ruichao; Xiong, Hanzhen; Qiu, Fuman; Liu, Shaoyan; Zhang, Minfen; Wang, Fang; Wang, Yu; Zhou, Xuan; Xiao, Guohong; Wang, Xudong; Jiang, Qingping

doi:10.1016/j.canlet.2016.09.019

Cited by 110 publications

(99 citation statements)

References 43 publications

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“…For instance, lncRNA SPRY4-IT1 acted as an endogenous sponge to downregulate the miR-101-3p expression in bladder cancer [26]. LncRNA MALAT1 could act as a ceRNA by directly binding to miR-200c and downregulating miR-200c expression in endometrioid endometrial carcinoma [27]. The same negative correlation and regulatory and control mechanisms also present in other lncRNA/miRNA, such as H19/miR-29a [28], SNHG6–003/miR-26a/b [29], and Unigene56159/miR-140-5p [30].…”

Section: Discussionmentioning

confidence: 99%

Long noncoding RNA MIR31HG inhibits hepatocellular carcinoma proliferation and metastasis by sponging microRNA-575 to modulate ST7L expression

Yan

Tang

Chen

et al. 2018

J Exp Clin Cancer Res

100

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BackgroundEmerging evidences have indicated that long noncoding RNAs (lncRNAs) play essential roles in the development and progression of cancers. Dysregulation of lncRNA MIR31HG has recently been reported in several types of cancers, and researches on the function of MIR31HG in cancers suggested that MIR31HG could act as either oncogene or tumor suppressor. But the functional involvement of MIR31HG has not been studied in hepatocellular carcinoma (HCC).MethodsIn this study, MTS assays, colony formation assay, Wound-healing assay, Transwell assy, and tumor xenografts experiments were used to identify biological effects of MIR31HG on HCC cells HCC proliferation and metastasis in vitro and in vivo. Dual-luciferase reporter assay and RNA immunoprecipitation (RIP) assay were performed to show the interactions of MIR31HG and miR-575. The bioinformatics methods were completed to find the target genes of miR-575. And Dual-luciferase reporter assay and Western blot analysis were further used to confirm the target gene of miR-575.ResultsWe found that overexpression of MIR31HG obviously suppressed HCC proliferation and metastasis in vitro and in vivo, whereas knockdown of MIR31HG had the opposite effects. Besides, overexpression of MIR31HG significantly decreased the expression of microRNA-575 (miR-575), which plays an oncogenic role in HCC. Moreover, dual-luciferase reporter assay and RNA immunoprecipitation (RIP) assay revealed that MIR31HG exerted tumor-suppressive functions by binding directly to miR-575, and there was a reciprocal inhibition between MIR31HG and miR-575 in the same RNA-induced silencing complex (RISC). Furthermore, overexpression of MIR31HG enhanced the expression of suppression of tumorigenicity 7 like (ST7L), which was identified as a downstream target gene of miR-575. Thus, MIR31HG positively regulated ST7L expression through sponging miR-575, and acted as tumor suppressor in HCC.ConclusionsOverall, our study illuminates the role of MIR31HG as a miRNA sponge in HCC, and sheds new light on lncRNA-directed diagnostics and therapeutics in HCC.Electronic supplementary materialThe online version of this article (10.1186/s13046-018-0853-9) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Long noncoding RNA MIR31HG inhibits hepatocellular carcinoma proliferation and metastasis by sponging microRNA-575 to modulate ST7L expression

Yan

Tang

Chen

et al. 2018

J Exp Clin Cancer Res

100

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“…When this interaction was altered, EMT was decreased, as well as the invasive capacity of RL-952 cells, leading to a reduced in vivo endometrioid endometrial cancer cell growth in a xenograft model [59]. …”

Section: Lncrnas With a Role In Several Cancers Of The Female Reprodumentioning

confidence: 99%

Dysregulated expression of long noncoding RNAs in gynecologic cancers

et al. 2017

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Cancers of the female reproductive system include ovarian, uterine, vaginal, cervical and vulvar cancers, which are termed gynecologic cancer. The emergence of long noncoding RNAs (lncRNAs), which are believed to play a crucial role in several different biological processes, has made the regulation of gene expression more complex. Although the function of lncRNAs is still rather elusive, their broad involvement in the initiation and progression of various cancers is clear. They are also involved in the pathogenesis of cancers of the female reproductive system. LncRNAs play a critical physiological role in apoptosis, metastasis, invasion, migration and cell proliferation in these cancers. Different expression profiles of lncRNAs have been observed in various types of tumors compared with normal tissues and between malignant and benign tumors. These differential expression patterns may lead to the promotion or suppression of cancer development and tumorigenesis. In the current review, we present the lncRNAs that show a differential expression between cancerous and normal tissues in ovarian, cervical and endometrial cancers, and highlight the associations between lncRNAs and some of the molecular pathways involved in these cancers.

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“…Application of miR-200 antagomirs can reverse EMT caused by the depletion of ERRα molecules, with an increase of Snail expression [68]. Many in vitro studies have strongly supported that miR-200s expression determines cellular expression of epithelial or mesenchymal biomarkers in endometrial [69] and ovarian cancer cell lines [40]. The resulting cells display distinct MET or EMT phenotypes [70].…”

Section: Mir-200 and Ovarian Cancermentioning

confidence: 99%

The Functions of MicroRNA-200 Family in Ovarian Cancer: Beyond Epithelial-Mesenchymal Transition

Choi

2017

IJMS

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The majority of studies on microRNA-200 family members (miR-200s) in human cancers are based on the premise that miR-200s maintain epithelial cell integrity by suppressing epithelial-mesenchymal transition (EMT) through direct inhibition of mesenchymal transcription factors zinc finger E-box-binding homeobox 1/2 (ZEB1/ZEB2) and transforming growth factor-β (TGF-β), a potent inducer of EMT. Hence, downregulation of miR-200 in cancer cells promotes EMT and cancer metastasis. Yet, miR-200s are highly expressed in ovarian cancer, and ovarian cancer metastasizes primarily by dissemination within the pelvic cavity. In this review, we will refocus the epithelial property of ovarian cancer cells and the role of miR-200s in safeguarding this property, as well as the diverse roles of miR-200s in inclusion cyst formation, cancer cell growth, collective movement, angiogenesis, exosome-mediated cell communication, and chemoresponse. Taken together, miR-200s play a significant role in the initiation, progression and metastasis of ovarian cancer and may serve as diagnostic biomarkers and a target in therapeutic development.

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Disrupting MALAT1/miR-200c sponge decreases invasion and migration in endometrioid endometrial carcinoma

Cited by 110 publications

References 43 publications

Long noncoding RNA MIR31HG inhibits hepatocellular carcinoma proliferation and metastasis by sponging microRNA-575 to modulate ST7L expression

Long noncoding RNA MIR31HG inhibits hepatocellular carcinoma proliferation and metastasis by sponging microRNA-575 to modulate ST7L expression

Dysregulated expression of long noncoding RNAs in gynecologic cancers

The Functions of MicroRNA-200 Family in Ovarian Cancer: Beyond Epithelial-Mesenchymal Transition

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