Sex estimation methods based on skeletal remains vary on the selection of skeletal element, data acquisition and statistical approach resulting in variable classification accuracies that are highly dependent on the sample population. The only exception of this rule seems is the os coxa that appears to differ consistently between males and females across the globe. Currently sex based on the os coxa can be easily by estimated taking ten measurements, input these in the DPS2 software and get a sex estimate and the probability of correct group assessment. The performance of the software is highly reliable as confirmed by a validation study by Brůžek and colleagues (2017). Yet, there are still many populations not represented in the reference sample used to develop the software. The current study aimed to validate DPS2 using a sample from Crete, Greece. A total of 133 os coxae were measured following instructions on DSP2. Data were used to estimate sex with the software and to create population specific formulae for the Greeks. DSP2 classified 117/133 (85.7%) of the sample with over 95% posterior probability (PP) of correct classification. Of the individuals classified with over 95% PP, only 3 were misclassified (2.6%).The best population specific formula only improved this percentage by 2.1% which indicates that DSP2 is a reliable tool for sex estimation in the Greek sample and it is recommended as method of choice in sex estimation of remains of unknown ancestry. If Greek ancestry is confirmed, population-specific formulae can be used in conjunction with DSP2 for a more reliable sex estimation.