Acid Red B dye wastewater was collaborative degradated by ozone and hydrogen peroxide. Various reaction conditions are studied which affect on decoloration rates of wastewater. The decoloration rate of Wastewater increases with O3 gas flow rate increasing, and also increases with pH value increasing. O3/H2O2 collaborative effects are better than O3 alone, and the decoloration rate is higher with more H2O2 addition. Ozone Oxidation have a good effect to degrade Acid Red B dye wastewater, the decoloration rate can reach 98% with inflating O3 30min. H2O2 synergy can greatly increase the reaction rate, shorten the reaction time, improve the utilization of ozone.