Disposable polydimethylsiloxane/silicon hybrid chips for protein detection

Li, Shifeng; Floriano, Pierre N.; Christodoulides, Nicolaos; Fozdar, David Y.; Shao, Dongbing; Ali, Mehnaaz F.; Dharshan, Priya; Mohanty, Sanghamitra; Neikirk, Dean P.; McDevitt, John T.; Chen, Shaochen

doi:10.1016/j.bios.2004.12.010

Cited by 30 publications

(22 citation statements)

References 25 publications

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“…In recent years, there has been a growing interest in inexpensive, self-contained diagnostic devices for use at the point-of-care, at home, and in resource-poor regions of the world, where sophisticated laboratory services are not readily available (Kim et al 2006; Wang et al 2006; Li et al 2005; Ho 2006; Ramachandran et al 2006; Lim and Zhang 2007; Chen et al 2007; Bhattacharyya and Klapperich 2007; Mauk et al 2007). Although home care tests such as dip-sticks and lateral flow immunochromatographic devices are widely available, they require the user to follow specific instructions and apply and blend reagents in a prescribed manner.…”

Section: Introductionmentioning

confidence: 99%

Finger-actuated, self-contained immunoassay cassettes

Qiu

Thompson

Chen

et al. 2009

Biomed Microdevices

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The building blocks for an inexpensive, disposable, luminescence-based microfluidic immunoassay cassette are described, and their integration in a point-of-care diagnostic system is demonstrated. Fluid motion in the cassette is driven by depressing finger-actuated pouches. All reagents needed for the immunoassay can be stored in the cassette in liquid form. Prior to use, the cassette consists of two separate parts. A top storage component contains pouches, sealed storage chambers, a metering chamber, and needle seats. The bottom processing component contains connection needles, a mixing chamber, and a detection chamber with immobilized proteins. Subsequent to sample introduction, the storage and processing components are mated. The needles form hydraulic connections between the two parts and, in some cases, close valves. The pouches are then actuated sequentially to induce flow of various reagents and facilitate process operations. The cassette is compatible with different detection modalities. Both a cassette with immunochromatographic-based detection and a cassette with microbead-based detection were constructed and evaluated. The immunochromatographic cassette was used to detect antibodies to HIV in saliva samples. The bead-based cassette was used to detect the proinflammatory chemokine IL-8. The experimental data demonstrates good repeatability and reasonable sensitivity.

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Section: Introductionmentioning

confidence: 99%

Finger-actuated, self-contained immunoassay cassettes

Qiu

Thompson

Chen

et al. 2009

Biomed Microdevices

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“…32 The pyramidal wells have been used by other researchers to trap atoms with lasers 8 and as microbeakers that contain bead-based chemical reactions for protein detection. 33,34 Mirrored pyramidal wells that are etched through the wafer offer additional opportunities for microfluidic flow applications and trans-illumination.…”

Section: Interpreting the Image: 3d Versus 2d Microscopymentioning

confidence: 99%

Micro-Mirrors for Nanoscale Three-Dimensional Microscopy

Seale¹,

Janetopoulos²,

Wikswo

2009

ACS Nano

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A research-grade optical microscope is capable of resolving fine structures in two-dimensional images. However, three-dimensional resolution, or the ability of the microscope to distinguish between objects lying above or below the focal plane from in-focus objects, is not nearly as good as in-plane resolution. In this issue of ACS Nano, McMahon et al. report the use of mirrored pyramidal wells with a conventional microscope for rapid, 3D localization and tracking of nanoparticles. Mirrors have been used in microscopy before, but recent work with MPWs is unique because it enables the rapid determination of the x-, y-, and z-position of freely diffusing nanoparticles and cellular nanostructures with unprecedented speed and spatial accuracy. As inexpensive tools for 3D visualization, mirrored pyramidal wells may prove to be invaluable aids in nanotechnology and engineering of nanomaterials.

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“…Rapid and simultaneous monitoring of target DNAs or proteins can be achieved in the miniaturized sample platform. Highly sensitive optical detection at these sample platforms generally depends on commercially available detectors such as microscope or microplate reader (Kukar et al 2002;Coleman et al 2004;Noerholm et al 2004;Li et al 2005;Wang et al 2006). However, these detectors are very expensive.…”

Section: Introductionmentioning

confidence: 99%

Low noise bipolar photodiode array protein chip based on on-chip bioassay for the detection of E. coli O157:H7

Kim

Yang²,

Kwon³

et al. 2007

Biomed Microdevices

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A bipolar photodiode array (PDA) protein chip is presented for the detection of E. coli O157:H7. Through unique design of the bipolar PDA microchip, the device was able to detect E. coli O157:H7 directly on the surface of the bipolar PDA. The bipolar PDA microchip maintained low noise level in the entire process of on-chip protein assay and demonstrated high performance of analog signal processing. At every reaction step of the on-chip bioassay, stability of wet photodiode detection elements was confirmed by monitoring the variance of their photosignals with respect to the irradiated red beam. The background signal represented less than 1.8% variance with respect to maximum signal of photodiode detection elements. As a result of using the on-chip bioassay, any complicated optical alignment and components could be removed in the constructed protein chip. This protein chip enables direct optical detection of E. coli O157:H7 eliminating the need of conventional expensive microplate reader that is incompatible with size of sampling platform of protein chip. The independence of the constructed protein chip on conventional microplate reader can contribute greatly to further miniaturization of protein chip and field usable lab-on-a-chip.

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Disposable polydimethylsiloxane/silicon hybrid chips for protein detection

Cited by 30 publications

References 25 publications

Finger-actuated, self-contained immunoassay cassettes

Finger-actuated, self-contained immunoassay cassettes

Micro-Mirrors for Nanoscale Three-Dimensional Microscopy

Low noise bipolar photodiode array protein chip based on on-chip bioassay for the detection of E. coli O157:H7

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