Disposable Pipette Extraction Followed by Direct MS/MS Analysis of Beta Amyloid Peptides (Aβ38, Aβ40, and Aβ42) in Cerebrospinal Fluid Samples
Caroline Grecco,
Eduardo José Crevelin,
Vitor Tumas
et al.
Abstract:Alzheimer’s disease (AD) is a neurodegenerative disorder characterized by extracellular accumulation of amyloid-β (Aβ) peptides in the brain. This study demonstrates the direct association of the disposable pipette extraction (DPX) with tandem mass spectrometry (MS/MS) for the analysis of Aβ peptides in cerebrospinal fluid (CSF) samples. Different parameters were optimized in order to improve detectability in the MS/MS including mobile phase percentage of ammonium hydroxide, mobile phase flow rate and acquisit… Show more
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