Disposable paper-based bipolar electrode array for multiplexed electrochemiluminescence detection of pathogenic DNAs

Feng, Qiumei; Chen, Hong‐Yuan; Xu, Jing‐Juan

doi:10.1007/s11426-014-5295-4

Cited by 28 publications

(11 citation statements)

References 41 publications

(28 reference statements)

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“…Wu et al fabricated a microfluidic paper-based ECL origami cyto-device in which aptamer-modified Au electrodes were used as a working electrode for cancer cells capture and cyto-sensing [192]. Additionally, a bipolar electrode (BPE) array was fabricated for ECL detection of pathogenic DNA [193]. This paper-based BPE device had 15 units, each having six sensing and two reporting cells designed by wax.…”

Section: Detection Techniques and Applicationsmentioning

confidence: 99%

Fabrication, Flow Control, and Applications of Microfluidic Paper-Based Analytical Devices

2019

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Paper-based microfluidic devices have advanced significantly in recent years as they are affordable, automated with capillary action, portable, and biodegradable diagnostic platforms for a variety of health, environmental, and food quality applications. In terms of commercialization, however, paper-based microfluidics still have to overcome significant challenges to become an authentic point-of-care testing format with the advanced capabilities of analyte purification, multiplex analysis, quantification, and detection with high sensitivity and selectivity. Moreover, fluid flow manipulation for multistep integration, which involves valving and flow velocity control, is also a critical parameter to achieve high-performance devices. Considering these limitations, the aim of this review is to (i) comprehensively analyze the fabrication techniques of microfluidic paper-based analytical devices, (ii) provide a theoretical background and various methods for fluid flow manipulation, and iii) highlight the recent detection techniques developed for various applications, including their advantages and disadvantages.

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Section: Detection Techniques and Applicationsmentioning

confidence: 99%

Fabrication, Flow Control, and Applications of Microfluidic Paper-Based Analytical Devices

2019

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“…To perform an electrochemiluminescence immunoassay with a μPAD for sensitive and multiplexed analysis, Ge et al constructed a three-dimensional electrochemiluminescence μPAD based on wax-patterned technology and screen-printed electrodes for the detection of four tumor markers in serum samples ( Figure 11 ) [ 151 ]. Electrochemiluminescence μPADs with bipolar electrodes have been used to promote both reduction and oxidation reactions without the need for an external power supply [ 153 , 154 , 156 ]. Zhang et al developed a bipolar electrochemiluminescence paper-based platform consisting of three electrodes integrated into parallel into a single device for rapid, high-throughput detection of different biomarkers [ 153 ].…”

Section: Detection Techniquesmentioning

confidence: 99%

Recent Advances in Microfluidic Paper-Based Analytical Devices toward High-Throughput Screening

et al. 2020

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Microfluidic paper-based analytical devices (µPADs) have become promising tools offering various analytical applications for chemical and biological assays at the point-of-care (POC). Compared to traditional microfluidic devices, µPADs offer notable advantages; they are cost-effective, easily fabricated, disposable, and portable. Because of our better understanding and advanced engineering of µPADs, multistep assays, high detection sensitivity, and rapid result readout have become possible, and recently developed µPADs have gained extensive interest in parallel analyses to detect biomarkers of interest. In this review, we focus on recent developments in order to achieve µPADs with high-throughput capability. We discuss existing fabrication techniques and designs, and we introduce and discuss current detection methods and their applications to multiplexed detection assays in relation to clinical diagnosis, drug analysis and screening, environmental monitoring, and food and beverage quality control. A summary with future perspectives for µPADs is also presented.

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“…Xu’s group [113] developed a multi-channel pBPE-ECL for multiplex detection of pathogenic DNA (Figure 6I). When a target gene specifically binds to the capture probe covalently modified onto the BPE, the single probes labeled by Pt nanoparticles are also captured by forming a “capture probe-target-detection probe” sandwich structure.…”

Section: Electrochemiluminescence (Ecl)-based Detection Of Pathogenmentioning

confidence: 99%

“…( I ) Multi-channel paper-based bipolar electrode based on Ru(bpy) 3 2+ and TPrA reaction for pathogenic DNA detection. Reproduced with permission from [113], published by Science China Press and Springer-Verlag Berlin Heidelberg, 2015. ( II ) Schematic of paper-based bipolar electrode electrochemiluminescence (pBPE-ECL) analysis system for sensitive detection of pathogenic bacteria.…”

Section: Figurementioning

confidence: 99%

Recent Advances in Electrochemiluminescence Sensors for Pathogenic Bacteria Detection

2019

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Pathogenic bacterial contamination greatly threats human health and safety. Rapidly biosensing pathogens in the early stage of infection would be helpful to choose the correct drug treatment, prevent transmission of pathogens, as well as decrease mortality and economic losses. Traditional techniques, such as polymerase chain reaction and enzyme-linked immunosorbent assay, are accurate and effective, but are greatly limited because they are complex and time-consuming. Electrochemiluminescence (ECL) biosensors combine the advantages of both electrochemical and photoluminescence analysis and are suitable for high sensitivity and simple pathogenic bacteria detection. In this review, we summarize recent advances in ECL sensors for pathogenic bacteria detection and highlight the development of paper-based ECL platforms in point of care diagnosis of pathogens.

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Disposable paper-based bipolar electrode array for multiplexed electrochemiluminescence detection of pathogenic DNAs

Cited by 28 publications

References 41 publications

Fabrication, Flow Control, and Applications of Microfluidic Paper-Based Analytical Devices

Fabrication, Flow Control, and Applications of Microfluidic Paper-Based Analytical Devices

Recent Advances in Microfluidic Paper-Based Analytical Devices toward High-Throughput Screening

Recent Advances in Electrochemiluminescence Sensors for Pathogenic Bacteria Detection

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