Disposable orbitally shaken TubeSpin bioreactor 600 for Sf9 cell cultivation in suspension

Characterizing the fluid dynamics of the inverted frustoconical shaking bioreactor

“…However, at “out of phase” operating conditions, the P/V L decreased as shaking speed increased, and the value of P/V L had a trend to increase as filling volume increasing at a constant shaking speed, which indicate that the “out of phase” state may have more damping effect on the power input of the IFSB‐5L with lower filling volumes. In the IFSB‐5L with the filling volume of 1 L at the shaking speed of 53 rpm, the highest P/V L of 330 W·m 3 was found, which is a typical value of P/V L used for an orbitally shaken animal cells culture . The CFD predicted P/V L values were compared with the experiment result and the difference was found below 10%, validating the CFD results (Figure ).…”

Studies on fluid dynamics of the flow field and gas transfer in orbitally shaken tubes

“…Single‐use cell culture technology is playing an important role in biopharmaceutical manufacturing because of the commercial availability of a wide variety of disposable bioreactors and of the need for greater flexibility in production processes . Disposable orbitally shaken bioreactors (OSRs) with working volumes from small (<2 L) to large scale (<2000 L) have been used to cultivate animal cells in suspension . In contrast to stirred‐tank bioreactors (STRs) that require the sparging of pure oxygen into the liquid phase, it is possible with OSRs to adequately supply oxygen for high‐density animal cell cultures with air only, providing the gas passively or actively into the head space over the liquid phase without a sparger.…”

“…[1][2][3][4] Disposable orbitally shaken bioreactors (OSRs) with working volumes from small (<2 L) to large scale (<2000 L) have been used to cultivate animal cells in suspension. [5][6][7][8][9][10] In contrast to stirred-tank bioreactors (STRs) that require the sparging of pure oxygen into the liquid phase, it is possible with OSRs to adequately supply oxygen for high-density animal cell cultures with air only, providing the gas passively or actively into the head space over the liquid phase without a sparger. Among the small-scale bioreactors for animal cell culture, 50-mL ventilated centrifuge tubes (OSR50) have been used for the high-density cultivation of Chinese hamster ovary (CHO) cells, the predominant mammalian cell host for commercial recombinant protein production in suspension culture, as well as other mammalian and insect cells.…”

Analyzing the suitability of a baffled orbitally shaken bioreactor for cells cultivation using the computational fluid dynamics approach

“…Recently, orbitally shaken bioreactors (OSRs) is becoming increasingly used for mammalian cells cultivation in suspension . The working volumes of OSRs are commercially available from 10 mL up to 200 L now . In contrast to stirred tanks (STRs), the OSRs have more simplified vessel structure which is more favorable in scale‐up process, and more gentle hydrodynamic stress environment which is more suitable to cultivate stress‐sensitive cells like Chinese hamster ovary cells (CHO).…”

“…In order to improve the efficiency of mixing and mass transfer in OSRs, different vessel geometries had been developed based on the cylindrical structure. For example, a conical bottom was designed for disposable 50 and 600‐mL orbitally shaken tubes (OSR50 and OSR600) to facilitate the purification process and a helical track was attached to the inside wall of a 30‐L OSR to enhance the level of oxygen transfer rate (OTR) . However, increasing the mixing and mass transfer normally has the penalty of generating high shear stress which is harmful to cells growth.…”