Disposable aptamer-sensor aided by magnetic nanoparticle enrichment for detection of salivary cortisol variations in obstructive sleep apnea patients

Fernandez, Renny Edwin; Umasankar, Yogeswaran; Manickam, Pandiaraj; Nickel, Jeffrey C.; Iwasaki, Laura R.; Kawamoto, Burt; Todoki, Kristen C.; Scott, Joanna; Bhansali, Shekhar

doi:10.1038/s41598-017-17835-8

Cited by 63 publications

(23 citation statements)

References 39 publications

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“…In addition, they can be designed for different degrees of affinity for a targeted molecule vs. a modified disturbing analog 40,41 . Moreover, aptamers are less affected by temperature fluctuations and are more stable for long term storage 18,42 . They can be covalently immobilized on most surfaces by modifying the 5′-or 3′-end 43 .…”

Section: Aptamersmentioning

confidence: 99%

“…Three different apatmers have been introduced for detection of the cortisol. They have 40 44 , 61 18 , and 85 38,45 nucleotides. The one with 85 nucleotides has been previously applied for a FET sensor with the detection limit of 50 nM 38 .…”

Section: Aptamersmentioning

confidence: 99%

“…Inkjetprinted electrochemical biosensors have been proposed by exploiting metalloporphyrin-based macrocyclic catalyst ink for the direct reduction of the cortisol captured by the aptamers-functionalized magnetic nanoparticles. However, the preparation of magnetic nanoparticles and catalyst inks are not yet fully mature for highperformance commercial sensors 18 . Other electrochemical methods are based on electrochemical impedance spectroscopy (EIS) or cyclic voltammetry via a mediator like Fe 3+ /Fe 4+ .…”

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confidence: 99%

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Extended gate field-effect-transistor for sensing cortisol stress hormone

et al. 2021

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Cortisol is a hormone released in response to stress and is a major glucocorticoid produced by adrenal glands. Here, we report a wearable sensory electronic chip using label-free detection, based on a platinum/graphene aptamer extended gate field effect transistor (EG-FET) for the recognition of cortisol in biological buffers within the Debye screening length. The device shows promising experimental features for real-time monitoring of the circadian rhythm of cortisol in human sweat. We report a hysteresis-free EG-FET with a voltage sensitivity of the order of 14 mV/decade and current sensitivity up to 80% over the four decades of cortisol concentration. The detection limit is 0.2 nM over a wide range, between 1 nM and 10 µM, of cortisol concentrations in physiological fluid, with negligible drift over time and high selectivity. The dynamic range fully covers those in human sweat. We propose a comprehensive analysis and a unified, predictive analytical mapping of current sensitivity in all regimes of operation.

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Section: Aptamersmentioning

confidence: 99%

Section: Aptamersmentioning

confidence: 99%

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confidence: 99%

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Extended gate field-effect-transistor for sensing cortisol stress hormone

et al. 2021

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“…Electrochemical sensors have been reported in the literature for the detection of cortisol, but only few of these are related to dogs [ 14 ]. Both antibodies and aptamers can be used to assemble cortisol sensors as can be seen in Table 1 [ 9 , 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 , 37 , 38 , 39 , 40 , 41 , 42 , 43 , 44 ].…”

Section: Introductionmentioning

confidence: 99%

Immunosensor for Assessing the Welfare of Trainee Guide Dogs

et al. 2021

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Cortisol is a well established biomarker hormone that regulates many processes in the body and is widely referred to as the stress hormone. Cortisol can be used as a stress marker to allow for detection of stress levels in dogs during the training process. This test will indicate if they will handle the stress under the training or if they might be more suitable as an assistant or companion dog. An immunosensor for detection of cortisol was developed using electrochemical impedance spectroscopy (EIS). The sensor was characterized using chemical and topographical techniques. The sensor was calibrated and its sensitivity determined using a cortisol concentration range of 0.0005 to 50 μg/mL. The theoretical limit of detection was found to be 3.57 fg/mL. When the immunosensor was tested on canine saliva samples, cortisol was detected and measured within the relevant physiological ranges in dogs.

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“…But they suffer from long detection time, lengthy sample preparation time and analysis, low sensitivity and cost. Recently, novel electrochemical immunosensor, aptamer sensor and imprinted sensor 21 , 22 method has been developed to detect cortisol owing to their fast analysis speed, high sensitivity, and low cost 7 , 23 , 24 . However, these sensors involve the use of traditional Au metallic disk, glassy carbon electrode (GCE), metal electrodes, metallic fibers or printed electrodes.…”

Section: Introductionmentioning

confidence: 99%

Carbon fiber based electrochemical sensor for sweat cortisol measurement

Sekar

Manickam

Bhansali

et al. 2019

Sci Rep

111

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This study examines the use of a conductive carbon fiber to construct a flexible biosensing platform for monitoring biomarkers in sweat. Cortisol was chosen as a model analyte. Functionalization of the conductive carbon yarn (CCY) with ellipsoidal Fe2O3 has been performed to immobilize the antibodies specific to cortisol. 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) and N-Hydroxysuccinimide (NHS) chemistry has been used to immobilize the antibodies onto the Fe2O3 modified CCY. Crystallinity, structure, morphology, flexibility, surface area, and elemental analysis were studied using X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), Raman spectroscopy, Field emission scanning electron microscopy with energy dispersive X-ray spectroscopy (FE-SEM/EDS) and Brunauer–Emmett–Teller (BET) analysis. Mechanical properties of the fiber such as tensile strength, young’s modulus have also been investigated. Under optimal parameters, the fabric sensor exhibited a good linearity (r2 = 0.998) for wide a linear range from 1 fg to 1 μg with a detection limit of 0.005 fg/mL for the sensitive detection of cortisol. Repeatability, reliability, reproducibility, and anti-interference properties of the current sensor have been investigated. Detection of cortisol levels in human sweat samples has also been investigated and the results were validated with commercial chemiluminescence immunoassay (CLIA) method.

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Disposable aptamer-sensor aided by magnetic nanoparticle enrichment for detection of salivary cortisol variations in obstructive sleep apnea patients

Cited by 63 publications

References 39 publications

Extended gate field-effect-transistor for sensing cortisol stress hormone

Extended gate field-effect-transistor for sensing cortisol stress hormone

Immunosensor for Assessing the Welfare of Trainee Guide Dogs

Carbon fiber based electrochemical sensor for sweat cortisol measurement

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