Displacement-type Quartz Crystal Microbalance Immunosensing Platform for Ultrasensitive Monitoring of Small Molecular Toxins

Tang, Dianping; Zhang, Bing; Tang, Juan; Hou, Li; Chen, Guonan

doi:10.1021/ac401599t

Cited by 56 publications

(42 citation statements)

References 44 publications

(71 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It was reported that a graphene functionalized sensing-based biosensor combined with a quartz crystal microbalance immunosensor was used t to detect BTX [82]. A dendrimer decorated with gold nanoparticles was used to fabricate electrochemical immunosensors to detect BTXs [83].…”

Section: The Detection Of Representative Marine Toxins By Aptasensorsmentioning

confidence: 99%

Marine Toxins Detection by Biosensors Based on Aptamers

Wei

Liu

Zhang

et al. 2019

Toxins

View full text Add to dashboard Cite

Marine toxins cause great harm to human health through seafood, therefore, it is urgent to exploit new marine toxins detection methods with the merits of high sensitivity and specificity, low detection limit, convenience, and high efficiency. Aptasensors have emerged to replace classical detection methods for marine toxins detection. The rapid development of molecular biological approaches, sequencing technology, material science, electronics and chemical science boost the preparation and application of aptasensors. Taken together, the aptamer-based biosensors would be the best candidate for detection of the marine toxins with the merits of high sensitivity and specificity, convenience, time-saving, relatively low cost, extremely low detection limit, and high throughput, which have reduced the detection limit of marine toxins from nM to fM. This article reviews the detection of marine toxins by aptamer-based biosensors, as well as the selection approach for the systematic evolution of ligands by exponential enrichment (SELEX), the aptamer sequences. Moreover, the newest aptasensors and the future prospective are also discussed, which would provide thereotical basis for the future development of marine toxins detection by aptasensors.Key Contribution: This article reviews systematically the toxicity of marine toxins, the development of aptarmer-based biosensors, and progress in the marine toxin detection by aptasensors.Toxins 2020, 12, 1 2 of 22 selection technology and biosensor fabrication technology offer a new solution for the detection of marine toxins with high efficiency and sensitivity [13][14][15][16][17].Marine toxins are a class of small molecular compounds mainly produced by red tide algae. In recent years, with the aggravation of environmental pollution, human deaths due to accidental ingestion of toxic shellfish are also often reported [18][19][20][21]. At present, the main methods for the detection of marine toxins include mouse bioassay (MBA) [22], enzyme-linked immunosorbent assay (ELISA) [23][24][25], high-performance liquid chromatography (HPLC) and liquid chromatography-mass spectrometry (LC-MS/MS), etc. [26][27][28]. However, these traditional methods have some drawbacks including the requirement of a technician, poor repeatability, expensive equipment, and issues related to animal ethics [22]. As a new detection technology, the biosensor is a kind of analysis systems using cell molecules and other bio-materials as sensitive elements, combined with a secondary sensor to detect a variety of chemicals by cascade amplified signal [29]. Because of their advantages of simple operation, high speed, high sensitivity, miniaturization, and easy automation, biosensors have been widely used in different fields including cell physiology [30], drug screening [31,32], food safety detection [33], disease diagnosis [34], etc.Aptamer, meaning "to fit", is a kind of oligonucleotide which can bind to target molecules with high selectivity and affinity. On account of the existence of aptamers in nature and the po...

show abstract

Section: The Detection Of Representative Marine Toxins By Aptasensorsmentioning

confidence: 99%

Marine Toxins Detection by Biosensors Based on Aptamers

Wei

Liu

Zhang

et al. 2019

Toxins

View full text Add to dashboard Cite

show abstract

“…Up to date, various methods and strategies based on different signal-transducer principles have been reported and developed for this purpose, e.g. by using surface plasmon resonance, quartz crystal microbalance, electrochemiluminescence, fluorescence (FL), chemiluminescence (CL), electrochemistry and colorimetric method891011121314. Typically, the colorimetric method is the most commonly used method in immunoassays because of several important advantages, e.g.…”

mentioning

confidence: 99%

Enhanced Colorimetric Immunoassay Accompanying with Enzyme Cascade Amplification Strategy for Ultrasensitive Detection of Low-Abundance Protein

Gao

Hou

et al. 2014

Sci Rep

Self Cite

141

114

View full text Add to dashboard Cite

Methods based on enzyme labels have been developed for colorimetric immunoassays, but most involve poor sensitivity and are unsuitable for routine use. Herein, we design an enhanced colorimetric immunoassay for prostate-specific antigen (PSA) coupling with an enzyme-cascade-amplification strategy (ECAS-CIA). In the presence of target PSA, the labeled alkaline phosphatase on secondary antibody catalyzes the formation of palladium nanostructures, which catalyze 3,3′,5,5′-tetramethylbenzidine-H2O2 system to produce the colored products, thus resulting in the signal cascade amplification. Results indicated that the ECAS-CIA presents good responses toward PSA, and allows detection of PSA at a concentration as low as 0.05 ng mL−1. Intra- and inter-assay coefficients of variation are below 9.5% and 10.7%, respectively. Additionally, the methodology is validated for analysis of clinical serum specimens with consistent results obtained by PSA ELISA kit. Importantly, the ECAS-CIA opens a new horizon for protein diagnostics and biosecurity.

show abstract

“…5B). Obviously, the LOD of our strategy was even lower than those of cardiomyocyte-based potential biosensor (1.5 ng mL -1 ) (Wang et al, 2015), enzyme-free electrochemical immunoassay (5 pg mL -1 ) (Lin et al, 2015b), aptamer-based competitive electrochemical biosensor (106 pg mL -1 ) (Eissa et al, 2015), Ag(I)-TMB-based colorimetric immunoassay (0.1 ng kg -1 ) (Lai et al, 2015), hollow gold nanosphere-based immunodipstick (0.1 ng mL -1 ) (Zhang et al, 2014), homogenous electrochemical immunoassay (6 pg mL -1 ) , displacement-type quartz crystal microbalance immunoassay (1.0 pg mL -1 ) (Tang et al, 2013), capillary electrophoresis-based immunoassay (0.1 ng mL -1 ) , glucose meter-based assay (0.1 ng mL -1 ) (Gao et al, 2014b) and commercialized BTB ELISA kit from Abraxis LLC Inc. (50 ng kg -1 ). Due to the legal limit of brevetoxin (≤ 80 μg of toxin per 100 g of shellfish tissue) (Hunt et al, 1979;Trainer et al, 1991), the Fenton reaction-based colorimetric immunoassay could completely meet the need of BTB monitoring in seafood.…”

Section: Analytical Performance Of Fenton Reaction-based Colorimetricmentioning

confidence: 99%

Fenton reaction-based colorimetric immunoassay for sensitive detection of brevetoxin B

Lai

Wei

Zhuang

et al. 2016

Biosensors and Bioelectronics

Self Cite

View full text Add to dashboard Cite

We designed a new colorimetric immunoassay for sensitive monitoring of brevetoxin B (BTB) using enzyme-controlled Fenton reaction with a high-resolution 3,3',5,5'-tetramethylbenzidine (TMB)-based visual colored system. Upon addition of hydrogen peroxide (H2O2), the equivalent iron(II) could be first converted into iron(III) and free hydroxyl radical (•OH) via the classical Fenton reaction. Then the as-produced iron(III) and •OH could cause a perceptible change from colorless to blue with the increasing H2O2 concentration in the presence of TMB. Based on Fenton reaction-triggered visual colored system, a novel competitive-type colorimetric enzyme immunoassay was developed for the quantitative screening of target BTB on the bovine serum albumin-BTB-modified magnetic bead using glucose oxidase/anti-BTB antibody-labeled gold nanoparticle as the signal-transduction tag. Upon target BTB introduction, the analyte competed with the conjugated BTB on the magnetic bead for anti-BTB antibody on gold nanoparticle. The carried glucose oxidase with the gold nanoparticle could implement the oxidation of glucose to produce H2O2, and the generated H2O2 promoted the above-mentioned Fenton reaction for color development. Under the optimal conditions, the absorbance decreased with the increasing target BTB in the range from 0.1 to 150 ng kg(-1) with a low detection limit (LOD) of 0.076 ng kg(-1). The LOD was 500-fold lower than that of commercialized Abraxis BTB ELISA kit. Non-specific adsorption was not observed. The precision, reproducibility and specificity were acceptable. Finally, the method accuracy was also validated for monitoring spiked seafood samples, giving results well matched with the referenced brevetoxin ELISA kit.

show abstract

Displacement-type Quartz Crystal Microbalance Immunosensing Platform for Ultrasensitive Monitoring of Small Molecular Toxins

Cited by 56 publications

References 44 publications

Marine Toxins Detection by Biosensors Based on Aptamers

Marine Toxins Detection by Biosensors Based on Aptamers

Enhanced Colorimetric Immunoassay Accompanying with Enzyme Cascade Amplification Strategy for Ultrasensitive Detection of Low-Abundance Protein

Fenton reaction-based colorimetric immunoassay for sensitive detection of brevetoxin B

Contact Info

Product

Resources

About