Dispersion of sulfur creates a valuable new growth medium formulation that enables earlier sulfur oxidation in relation to iron oxidation in <i>Acidithiobacillus ferrooxidans</i> cultures

Inaba, Yuta; Kernan, Timothy; West, Alan C.; Banta, Scott

doi:10.1002/bit.27847

Cited by 15 publications

(9 citation statements)

References 68 publications

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“…It is worth noticing that sulfur compounds have higher energy yields than Fe(II) since they have more electrons available (37). Although, A. ferrooxidans is believed to exhibit a preference for iron (38), evidence shows the simultaneous expression of genes forconsumption of iron and sulfur (39), being solubilization of sulfur compounds key to consuming both energy sources (40). This result is consistent with our analysis, where a preference for Fe(II) oxidation is only displayed when its availability surpasses thiosulfate.…”

Section: Resultsmentioning

confidence: 99%

Unveiling abundance-dependent metabolic phenotypes of microbial communities

Jiménez

Acuña

Cortés

et al. 2023

Preprint

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Constraint-based modeling has risen as an alternative for characterizing the metabolism of communities. Adaptations of Flux Balance Analysis have been proposed to model metabolic interactions in most cases, considering a unique optimal flux distribution derived from the maximization of biomass production. However, these approaches do not consider the development of other potentially novel essential functions not directly related to cell growth which forces them to display suboptimal growth rates in nature. Additionally, suboptimal states allow a degree of plasticity in the metabolism, thus allowing quick shifts between alternative flux distributions as an initial response to environmental changes. In this work, we present a method to explore the abundance-growth space as a representation of metabolic flux distributions of a community. This space is defined by the composition of a community, represented by its members' relative abundance and their growth rate. The analysis of this space allows us to represent the whole set of feasible fluxes without needing a complete description of the solution space unveiling abundance-dependent metabolic phenotypes displayed in a given environment. As an illustration, we consider a community composed of two bioleaching bacteria,Acidithiobacillus ferrooxidansWenelen andSulfobacillus thermosulfidooxidansCutipay, finding that changes in the composition of their available resources significantly affects their metabolic plasticity.

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Section: Resultsmentioning

confidence: 99%

Unveiling abundance-dependent metabolic phenotypes of microbial communities

Jiménez

Acuña

Cortés

et al. 2023

Preprint

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“…ferrooxidans cells with an initial optical density at 600 nm (OD600) of 0.001 corresponding to a cell density of 8.3 × 10 6 cells/mL were cultured in 100 mL of F2S and incubated in a shaking incubator (30 °C and 150 rpm). The F2S growth medium (pH 1.8) contains 0.8 g/L (NH 4 ) 2 SO 4 , 0.1 g/L HK 2 PO 4 , 2.0 g/L MgSO 4 ·7H 2 O, 5 mL/L trace element solution (MD-TMS; ATCC), 10 mM citric acid, 100 mM FeSO 4 ·7H 2 O, and 0.1% (w/v) dispersed sulfur (#S789400; Toronto Research Chemicals, Toronto, ON) . The grown cells were harvested at the stationary phase (120 h) by centrifugation (5,000 g for 7 min), and sulfur and iron precipitates were removed by low-speed centrifugation (3,000 g for 10 s).…”

Section: Methodsmentioning

confidence: 99%

Overexpression of AcoP in Acidithiobacillus ferrooxidans for Enhanced Copper Reclamation

Jung,

Kim,

Inaba

et al. 2023

ACS EST Eng.

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Copper is an essential critical metal that will be indispensable as we work to reduce our dependence on fossil fuel sources and move to electrification. Here, we explored the cellular overexpression of the AcoP protein to enhance copper binding ability of a representative bioleaching acidophile, Acidithiobacillus ferrooxidans. The engineered cells exhibited enhanced copper binding, regardless of the oxidation state of copper (1.5-fold improvement), as well as in the presence of a nontarget metal, iron (up to 2.7-fold improvement). The acid-and metal-tolerant physiology of A. ferrooxidans enabled the cells to grow in the presence of bottom ash produced from a municipal waste-to-energy facility, and the genetically engineered cells also exhibited enhanced copper recovery (9.5-fold higher selectivity) from this nonconventional feedstock. This work demonstrates a promising proof of concept where bioleaching cells can be modified for the effective reclamation of critical materials from different sources for emerging future applications.

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“…The grown cells were harvested by centrifugation (5000 g for 7 min). The F2S growth medium (pH 1.8) contains 0.8 g/L (NH 4 ) 2 SO 4 , 0.1 g/L HK 2 PO 4 , 2.0 g/L MgSO 4 ·7H 2 O, 5 mL/L trace mineral solution (MD-TMS), 10 mM citric acid, 100 mM FeSO 4 ·7H 2 O, and 0.1% (w/v) dispersed sulfur (#S789400; Toronto Research Chemicals, Toronto, ON) . The media were sterilized through a filter with a 0.2 mm pore size (Thermo Fisher Scientific, Waltham, MA).…”

Section: Methods and Materialsmentioning

confidence: 99%

Engineering Polyhistidine Tags on Surface Proteins of Acidithiobacillus ferrooxidans: Impact of Localization on the Binding and Recovery of Divalent Metal Cations

Jung

Inaba

Jiang

et al. 2022

ACS Appl. Mater. Interfaces

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Metal processing using microorganisms has many advantages including the potential for reduced environmental impacts as compared to conventional technologies. Acidithiobacillus ferrooxidansis an iron-and sulfur-oxidizing chemolithoautotroph that is known to participate in metal bioleaching, and its metabolic capabilities have been exploited for industrial-scale copper and gold biomining. In addition to bioleaching, microorganisms could also be engineered for selective metal binding, enabling new opportunities for metal bioseparation and recovery. Here, we explored the ability of polyhistidine (polyHis) tags appended to two recombinantly expressed endogenous proteins to enhance the metal binding capacity of A. ferrooxidans. The genetically engineered cells achieved enhanced cobalt and copper binding capacities, and the Langmuir isotherm captures their interaction behavior with these divalent metals. Additionally, the cellular localization of the recombinant proteins correlated with kinetic modeling of the binding interactions, where the outer membrane-associated polyHis-tagged licanantase peptide bound the metals faster than the periplasmically expressed polyHis-tagged rusticyanin protein. The selectivity of the polyHis sequences for cobalt over copper from mixed metal solutions suggests potential utility in practical applications, and further engineering could be used to create metal-selective bioleaching microorganisms.

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Dispersion of sulfur creates a valuable new growth medium formulation that enables earlier sulfur oxidation in relation to iron oxidation in Acidithiobacillus ferrooxidans cultures

Cited by 15 publications

References 68 publications

Unveiling abundance-dependent metabolic phenotypes of microbial communities

Unveiling abundance-dependent metabolic phenotypes of microbial communities

Overexpression of AcoP in Acidithiobacillus ferrooxidans for Enhanced Copper Reclamation

Engineering Polyhistidine Tags on Surface Proteins of Acidithiobacillus ferrooxidans: Impact of Localization on the Binding and Recovery of Divalent Metal Cations

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