Dispersing behaviour of T. infestans: evidence from a genetical study of field populations in Bolivia

Dujardin, Jean‐Pierre; Fuente, C. La; Cardozo, L.; Tibayrenc, Michel

doi:10.1590/s0074-02761988000500042

Cited by 10 publications

(7 citation statements)

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“…The "isolation by distance model," Þrst proposed by Dujardin et al (1988) and conÞrmed for T. infestans (Breniere et al 1998), may partially explain the increasing genetic distance with geographic distance for T. dimidiata domestic populations (separated by Ͻ100 km). A correlation between genetic and geographical distances was observed for populations of Triatoma brasiliensis Neiva 1911 by using RAPD-PCR (Borges et al 2000).…”

Section: Discussionmentioning

confidence: 91%

A Preliminary Assessment of Genetic Differentiation ofTriatoma dimidiata(Hemiptera: Reduviidae) in Guatemala by Random Amplification of Polymorphic DNA-Polymerase Chain Reaction

Calderón¹,

Dorn²,

Melgar³

et al. 2004

J Med Entomol

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The population genetics of Triatoma dimidiata (Latreille, 1811) from five different provinces in Guatemala, including three sylvan and three domestic populations, was investigated by random amplification of polymorphic DNA-polymerase chain reaction. There is a high degree of genetic variation in all of the T. dimidiata populations as evidenced by high levels of average expected heterozygosity and polymorphism. Domestic populations are more closely related to each other (D = 0.05-0.085, Nei's genetic distance) than are the sylvan (D = 0.121-0.189). Within the limited sample size of three populations, there was a correlation with geographic and genetic distance for the domestic populations, but not for the sylvan. Surprisingly, one of the sylvan populations was genetically very similar to the domestic populations. The FST demonstrated a high degree of differentiation at the country-wide level (FST = 0.175) and a moderate degree of differentiation within the sylvan (FST = 0.135) or domestic (FST = 0.097) populations. Although these results demonstrated that gene flow is limited between different provinces in Guatemala, hierarchical analysis showed that barriers between the Atlantic and Pacific drainage slopes were not biologically significant limiters of gene flow.

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Section: Discussionmentioning

confidence: 91%

A Preliminary Assessment of Genetic Differentiation ofTriatoma dimidiata(Hemiptera: Reduviidae) in Guatemala by Random Amplification of Polymorphic DNA-Polymerase Chain Reaction

Calderón¹,

Dorn²,

Melgar³

et al. 2004

J Med Entomol

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“…Variation in population structure among regions was established using twelve isozymes [9],[30]. There was significant differentiation of T. infestans populations between villages located 50 Km apart in Vallegrande, Santa Cruz yet in the Yungas of La Paz, populations only a few Km apart showed significant differences.…”

Section: Discussionmentioning

confidence: 99%

Microsatellites Reveal a High Population Structure in Triatoma infestans from Chuquisaca, Bolivia

2008

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BackgroundFor Chagas disease, the most serious infectious disease in the Americas, effective disease control depends on elimination of vectors through spraying with insecticides. Molecular genetic research can help vector control programs by identifying and characterizing vector populations and then developing effective intervention strategies.Methods and FindingsThe population genetic structure of Triatoma infestans (Hemiptera: Reduviidae), the main vector of Chagas disease in Bolivia, was investigated using a hierarchical sampling strategy. A total of 230 adults and nymphs from 23 localities throughout the department of Chuquisaca in Southern Bolivia were analyzed at ten microsatellite loci. Population structure, estimated using analysis of molecular variance (AMOVA) to estimate FST (infinite alleles model) and RST (stepwise mutation model), was significant between western and eastern regions within Chuquisaca and between insects collected in domestic and peri-domestic habitats. Genetic differentiation at three different hierarchical geographic levels was significant, even in the case of adjacent households within a single locality (R ST = 0.14, F ST = 0.07). On the largest geographic scale, among five communities up to 100 km apart, R ST = 0.12 and F ST = 0.06. Cluster analysis combined with assignment tests identified five clusters within the five communities.ConclusionsSome houses are colonized by insects from several genetic clusters after spraying, whereas other households are colonized predominately by insects from a single cluster. Significant population structure, measured by both R ST and F ST, supports the hypothesis of poor dispersal ability and/or reduced migration of T. infestans. The high degree of genetic structure at small geographic scales, inferences from cluster analysis and assignment tests, and demographic data suggest reinfesting vectors are coming from nearby and from recrudescence (hatching of eggs that were laid before insecticide spraying). Suggestions for using these results in vector control strategies are made.

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“…Multilocus enzyme electrophoresis data have provided important information on gene ßow and genetic structuring in domestic T. infestans populations. In Bolivia, the panmictic unit was suggested to be a group of villages separated by 50 km in Vallegrande, and single villages separated only by few kilometers in the Yungas valleys (Dujardin et al 1988(Dujardin et al , 1998b. In other areas of Bolivia, however, the panmictic unit was represented by a single house or chicken coop when larger numbers of bugs were analyzed (Breniè re et al 1998).…”

Section: Discussionmentioning

confidence: 99%

Spatial Structuring ofTriatoma infestans(Hemiptera, Reduviidae) Populations from Northwestern Argentina Using Wing Geometric Morphometry

et al. 2004

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Wing geometric morphometry was used to study the spatial structuring of populations of Triatoma infestans from different villages, ecotopes, and sites within a village in northwestern Argentina. A total of 308 male and 197 female wings of T. infestans collected from peridomestic and domestic ecotopes in March 2000 was analyzed. On average, female bugs had a significantly larger wing size than males. Triatomines collected from domiciles or structures associated with chickens had larger wings than bugs collected from goat or pig corrals. The wing size of bugs did not differ significantly between villages. Discriminant analyses of wing shape showed significant divergence between villages, ecotopes, and individual collection sites. The study of metric variation of males between sites belonging to the same ecotope also revealed significant heterogeneity. Indeed, within the same section of the village the difference between two goat corrals was sometimes greater than that between neighboring goat and pig corrals. Thus, morphometric heterogeneity within villages may be the result not only of ecotope and host associations, but also of physical isolation between subunits. The strong structuring of T. infestans populations in the study area indicates that recolonization could be traced back to a small geographic source.

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Dispersing behaviour of T. infestans: evidence from a genetical study of field populations in Bolivia

Cited by 10 publications

References 0 publications

A Preliminary Assessment of Genetic Differentiation ofTriatoma dimidiata(Hemiptera: Reduviidae) in Guatemala by Random Amplification of Polymorphic DNA-Polymerase Chain Reaction

A Preliminary Assessment of Genetic Differentiation ofTriatoma dimidiata(Hemiptera: Reduviidae) in Guatemala by Random Amplification of Polymorphic DNA-Polymerase Chain Reaction

Microsatellites Reveal a High Population Structure in Triatoma infestans from Chuquisaca, Bolivia

Spatial Structuring ofTriatoma infestans(Hemiptera, Reduviidae) Populations from Northwestern Argentina Using Wing Geometric Morphometry

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