Dispersed growth of Streptomyces in liquid culture

Hobbs, Glyn; Frazer, Catherine M.; Gardner, David C.; Cullum, John; Oliver, Stephen G.

doi:10.1007/bf00258408

Cited by 247 publications

(161 citation statements)

References 12 publications

Supporting

Mentioning

155

Contrasting

Unclassified

Order By: Relevance

“…Protoplast preparation and transformation were as described by Hopwood et al (1985). SFM medium (mannitol, 20 g l ¹1 ; soya flour, 20 g l ¹1 ; agar, 20 g l ¹1 , dissolved in tap water and autoclaved twice) is a modified version of that reported by Hobbs et al (1989) and was used to make spore suspensions. Liquid minimal medium (NMMP; Hopwood et al, 1985) containing 1% (w/v) glucose, mannitol, maltose or maltose plus glucose, and unless otherwise stated 0.05% (w/v) casamino acids, or SMM (Strauch et al, 1992) containing 0.1% (w/v) casamino acids and 1% (w/v) glucose or maltose, were used to assess carbon-source utilization and for RNA isolation.…”

Section: Methodsmentioning

confidence: 99%

**Substrate induction and glucose repression of maltose utilization by Streptomyces coelicolor A3(2) is controlled by malR, a member of the lacI–galR family of regulatory genes**

Wezel

White

Young

et al. 1997

Molecular Microbiology

View full text Add to dashboard Cite

SummarymalR of Streptomyces coelicolor A3(2) encodes a homologue of the LacI/GalR family of repressor proteins, and is divergently transcribed from the malEFG gene cluster, which encodes components of an ATPdependent transport system that is required for maltose utilization. Transcription of malE was induced by maltose and repressed by glucose. Disruption or deletion of malR resulted in constitutive, glucoseinsensitive malE transcription at a level markedly above that observed in the parental malR þ strain, and overproduction of MalR prevented growth on maltose as carbon source. Consequently, MalR plays a crucial role in both substrate induction and glucose repression of maltose utilization. malR is expressed from a single promoter with transcription initiating at the first G of the predicted GTG translation start codon.

show abstract

Section: Methodsmentioning

confidence: 99%

**Substrate induction and glucose repression of maltose utilization by Streptomyces coelicolor A3(2) is controlled by malR, a member of the lacI–galR family of regulatory genes**

Wezel

White

Young

et al. 1997

Molecular Microbiology

View full text Add to dashboard Cite

show abstract

“…coelicolor in chemically defined growth media. We (Hobbs et al, 1989) have recently devised a medium which overcomes many of these difficulties and allows the dispersed growth of S . coelicolor under chemically defined conditions.…”

Section: Introductionmentioning

confidence: 99%

Pigmented antibiotic production by Streptomyces coelicolor A3(2): kinetics and the influence of nutrients

Hobbs¹,

Frazer²,

Gardner

et al. 1990

Journal of General Microbiology

View full text Add to dashboard Cite

~_ _ _ _ _ _~ _ _ _ _ _ _ _~The production of the pigments actinorhodin and undecylprodigiosin by Stveptomyces coelicolor A3(2) was examined in a chemically defined medium which permits dispersed growth of the organism. The physiological controls on the production of the two pigments were markedly disparate. Actinorhodin production occurred mainly in the stationary phase of batch cultures grown with glucose and sodium nitrate as the principal carbon and nitrogen sources. In the same batch cultures, undecylprodigiosin accumulated during the exponential growth phase. The production of both pigments was sensitive to the levels of ammonium and phosphate in the medium. Actinorhodin production was exquisitely sensitive to ammonium concentration, and was completely inhibited by as little as 1 mM-ammonium chloride, whereas more than 50 mhl-amnonium chloride was required to prevent undecylprodigiosin production. A similar, but less extreme effect was seen with phosphate: actinorhodin production was completely inhibited by 24 mM-phosphate, whereas undecylprodigiosin was still formed at this high phosphate concentration. The effects of ammonium inhibition of pigmented antibiotic production were relieved by reducing the concentration of phosphate in the medium, but changing the ammonium concentration had no effect on phosphate inhibition. Thus the regulation of pigment production by these two nutrients is interrelated, with phosphate control being epistatic to that of ammonium. The results implicate a phosphorylated intermediate as a major regulator of secondary metabolite synthesis by S. coelicolor.

show abstract

“…[36] Tristhiazolylpyridin 42, [37] (57), [36] 1,2-(Z)-Methoxycarbonyl-1,3-bis(trimethyl-silyloxy)-1,3-butadien (65), [37] 2-Nitro-6-(2'-tetrahydropyranyloxymethyl)phenyl-brenztrauben-säureethylester (75), [153] 3-Hydrox-ypyridin-5,6-bis(trideutero-methoxycarbonyl)-2-carbon-säuremethylester (97-d 6 ), [241] 131 3-Hydroxypicolinsäuremethylester (100), [278] L-Boc-Thr(TBS)-OH (140), [201] L-FmocThr(TBS)-OH (143), [202] 2'-Bromacetyl-pyridin (161), [219] 6-Brom-3-hydroxypicolinsäure-methyl-ester (204), [267] 4-(Tributyl-stannyl)-1-trityl-1H-imidazol (217), [269] 4,6-Dibrom-3-hydroxy-picolinsäure-diemethylester (228), [278] Dess-Martin-Periodinan, [312] 3-Hydroxypyridin-2,5,6-tricarbonsäure-trimethylester, [37] 3-Meth-oxypicolinsäuremethyl-ester(240) [308] Indol-2-boron-säurepinakolester, [313] Indol-3-boronsäurepinakol-ester, [314] 1H-Pyrrol-3-boronsäure-pinakolester, [315] 1-(Tetrahydro-2H-pyran-2-yl)-1H-pyrazol-5-boronsäurepinakol-ester, [268] Pyrazol-4-boronsäurepinakolester, [314] (R)-2-Azido-3-(tritylthio)-propansäureallyl-ester, [37] PyDOP. [225] Dichlormethan [316] die Sporen wurden anschließend geerntet und in Glycerol gelagert. [317] Hemmhoftest Eine S. coelicolor-Sporenlösung (100 µL, 1×10 8 aktive Sporen/ml) wurde auf Petrischalen gleichmäßig verteilt (8.5 cm) die 30 mL Minimal-Medium enthielten.…”

Section: Synthese Der Nicht Fluorierten Biscarboxamid-ligandenunclassified

Totalsynthese von Nosiheptid

Wojtas

Riedrich

et al. 2016

Angewandte Chemie

View full text Add to dashboard Cite

Eine Totalsynthese des bismakrocyclischen Thiopeptidantibiotikums Nosiheptid gelang durch den Aufbau einer voll funktionalisierten linearen Vorstufe und konsekutive Makrocyclisierungen. Schlüsselmerkmale waren eine kritische Makrothiolactonisierung und eine milde Entschützungsstrategie für den 3‐Hydroxypyridin‐Kern. Der Naturstoff war mit isoliertem authentischem Material im Hinblick auf spektroskopische Daten und antimikrobielle Aktivität identisch.

show abstract

Dispersed growth of Streptomyces in liquid culture

Cited by 247 publications

References 12 publications

**Substrate induction and glucose repression of maltose utilization by Streptomyces coelicolor A3(2) is controlled by malR, a member of the lacI–galR family of regulatory genes**

**Substrate induction and glucose repression of maltose utilization by Streptomyces coelicolor A3(2) is controlled by malR, a member of the lacI–galR family of regulatory genes**

Pigmented antibiotic production by Streptomyces coelicolor A3(2): kinetics and the influence of nutrients

Totalsynthese von Nosiheptid

Contact Info

Product

Resources

About