Dispersal of wild-type and genetically-modified Pseudomonas spp from treated seeds or soil to aerial parts of radish plants

Raaijmakers, Jos M.; Sluis, I. van der; Hout, M. Van; Bakker, Peter A. H. M.; Schippers, B.

doi:10.1016/0038-0717(95)00065-m

Cited by 13 publications

(5 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These results are similar to those reported in endophyte studies on sweet corn, cotton, red bean, and tomato plants with the same strains used in this study (McInroy and Kloepper 1995;Hallman et al 1997) and to reports by Kluepfel (1993) that Pseudomonas aureofaciens L11 readily moved into leaves and stems of corn, wheat, and soybean through vascular systems. As suggested by Raaijmakers et al (1995) and Kluepfel (1993), populations in aboveground portions might also be due to contact with bacterial cells when seed germinated in soilless medium.…”

Section: Discussionmentioning

confidence: 83%

Survival and colonization of rhizobacteria in a tomato transplant system

Yan

Reddy²,

Kloepper³

2003

Can. J. Microbiol.

View full text Add to dashboard Cite

Plant-growth-promoting rhizobacteria (PGPR) are used on crops most often as seed treatments; however, an alternative application method for transplanted vegetables is mixing PGPR into the soilless medium in which the transplants are grown. Studies were undertaken to compare root colonization and persistence of rifampicin-resistant mutants of PGPR strains Bacillus pumilus SE34 and Pseudomonas fluorescens 89B61, SE34r and 89B61r, on tomato as a function of application method. When the bacteria were incorporated into Promix soilless medium at log 6, 7, and 8 colony- forming units/g, populations of strain SE34r per gram of medium maintained the initial inoculum densities, while populations of 89B61r decreased approximately one to two orders of magnitude by 4 weeks after planting. The populations of each PGPR strain colonizing roots after application into the soilless medium showed a similar pattern at 6 weeks as that at 4 weeks after planting, with higher populations on the whole roots and lateral roots than on the taproots. Strain SE34r but not 89B61r moved upwards and colonized the phyllosphere when incorporated into the soilless medium. Following application as seed treatment, populations of SE34r were significantly higher on upper roots and on the taproot than were populations following application through the soilless medium. Conversely, populations were higher on lower roots and lateral roots following application through the soilless medium than were populations following application as seed treatment. While strain SE34 enhanced plant growth with application both to the medium and as seed treatment, the level of growth promotion was significantly greater with application in the soilless medium. The results indicate that PGPR can be successfully incorporated into soilless media in vegetable transplant production systems.

show abstract

Section: Discussionmentioning

confidence: 83%

Survival and colonization of rhizobacteria in a tomato transplant system

Yan

Reddy²,

Kloepper³

2003

Can. J. Microbiol.

View full text Add to dashboard Cite

show abstract

“…6). The presence of the introduced bacterial strains on and in cotelydons, but not on and in true leaves of tomato plants, is most likely the result of passive colonization/contamination of the cotyledons during germination of the bacteria‐treated seeds and subsequent seedling emergence (Raaijmakers et al ., 1995). These results indicate that massetolide A contributes to colonization of tomato plants by P. fluorescens SS101 and extend the findings of Nielsen et al .…”

Section: Discussionmentioning

confidence: 99%

Role of the cyclic lipopeptide massetolide A in biological control of Phytophthora infestans and in colonization of tomato plants by Pseudomonas fluorescens

et al. 2007

Self Cite

View full text Add to dashboard Cite

Summary• Pseudomonas strains have shown promising results in biological control of late blight caused by Phytophthora infestans . However, the mechanism(s) and metabolites involved are in many cases poorly understood. Here, the role of the cyclic lipopeptide massetolide A of Pseudomonas fluorescens SS101 in biocontrol of tomato late blight was examined.• Pseudomonas fluorescens SS101 was effective in preventing infection of tomato ( Lycopersicon esculentum ) leaves by P. infestans and significantly reduced the expansion of existing late blight lesions. Massetolide A was an important component of the activity of P. fluorescens SS101, since the massA -mutant was significantly less effective in biocontrol, and purified massetolide A provided significant control of P. infestans , both locally and systemically via induced resistance.• Assays with nahG transgenic plants indicated that the systemic resistance response induced by SS101 or massetolide A was independent of salicylic acid signalling. Strain SS101 colonized the roots of tomato seedlings significantly better than its massAmutant, indicating that massetolide A was an important trait in plant colonization.• This study shows that the cyclic lipopeptide surfactant massetolide A is a metabolite with versatile functions in the ecology of P. fluorescens SS101 and in interactions with tomato plants and the late blight pathogen P. infestans .

show abstract

“…The migratory response of bacterial inoculants to compounds released by plant roots is often the first step required for establishment of the bacteria in the rhizosphere and rhizoplane (29,48,50). Following the initial colonization by introduced bacteria, these organisms may spread further to the aerial parts of the plant (35). The degree of root colonization by bacterial inoculants depends on, in addition to the mode of application (35), factors intrinsic to the organism used, like the presence of flagella (11) and/or the presence of particular outer membrane lipopolysaccharides (13).…”

mentioning

confidence: 99%

“…Following the initial colonization by introduced bacteria, these organisms may spread further to the aerial parts of the plant (35). The degree of root colonization by bacterial inoculants depends on, in addition to the mode of application (35), factors intrinsic to the organism used, like the presence of flagella (11) and/or the presence of particular outer membrane lipopolysaccharides (13). Such features may differ from strain to strain.…”

mentioning

confidence: 99%

Endophytic Colonization of Potato ( Solanum tuberosum L.) by a Novel Competent Bacterial Endophyte, Pseudomonas putida Strain P9, and Its Effect on Associated Bacterial Communities

Andreote

Araújo

Azevedo

et al. 2009

Appl Environ Microbiol

103

View full text Add to dashboard Cite

Pseudomonas putida strain P9 is a novel competent endophyte from potato. P9 causes cultivar-dependent suppression of Phytophthora infestans. Colonization of the rhizoplane and endosphere of potato plants by P9 and its rifampin-resistant derivative P9R was studied. The purposes of this work were to follow the fate of P9 inside growing potato plants and to establish its effect on associated microbial communities. The effects of P9 and P9R inoculation were studied in two separate experiments. The roots of transplants of three different cultivars of potato were dipped in suspensions of P9 or P9R cells, and the plants were planted in soil. The fate of both strains was followed by examining colony growth and by performing PCR-denaturing gradient gel electrophoresis (PCR-DGGE). Colonies of both strains were recovered from rhizoplane and endosphere samples of all three cultivars at two growth stages. A conspicuous band, representing P9 and P9R, was found in all Pseudomonas PCR-DGGE fingerprints for treated plants. The numbers of P9R CFU and the P9R-specific band intensities for the different replicate samples were positively correlated, as determined by linear regression analysis. The effects of plant growth stage, genotype, and the presence of P9R on associated microbial communities were examined by multivariate and unweighted-pair group method with arithmetic mean cluster analyses of PCR-DGGE fingerprints. The presence of strain P9R had an effect on bacterial groups identified as Pseudomonas azotoformans, Pseudomonas veronii, and Pseudomonas syringae. In conclusion, strain P9 is an avid colonizer of potato plants, competing with microbial populations indigenous to the potato phytosphere. Bacterization with a biocontrol agent has an important and previously unexplored effect on plant-associated communities.The colonization of plant tissue is of paramount importance for successful application of plant-growth-promoting bacteria. For instance, efficient root colonization was shown to be important for the control of Fusarium oxysporum in tomato by a phenazine-1-carboxamide-producing Pseudomonas chlororaphis strain (6). The migratory response of bacterial inoculants to compounds released by plant roots is often the first step required for establishment of the bacteria in the rhizosphere and rhizoplane (29,48,50). Following the initial colonization by introduced bacteria, these organisms may spread further to the aerial parts of the plant (35). The degree of root colonization by bacterial inoculants depends on, in addition to the mode of application (35), factors intrinsic to the organism used, like the presence of flagella (11) and/or the presence of particular outer membrane lipopolysaccharides (13). Such features may differ from strain to strain. Once attached to plant roots, the inoculant bacteria may evoke "protective" responses in the plants, enabling them to resist phytopathogen attack (4).Endophytic colonization, characterized by colonization of internal plant tissues concomitant with growth and systemic spread, can ...

show abstract

Dispersal of wild-type and genetically-modified Pseudomonas spp from treated seeds or soil to aerial parts of radish plants

Cited by 13 publications

References 29 publications

Survival and colonization of rhizobacteria in a tomato transplant system

Survival and colonization of rhizobacteria in a tomato transplant system

Role of the cyclic lipopeptide massetolide A in biological control of Phytophthora infestans and in colonization of tomato plants by Pseudomonas fluorescens

Endophytic Colonization of Potato ( Solanum tuberosum L.) by a Novel Competent Bacterial Endophyte, Pseudomonas putida Strain P9, and Its Effect on Associated Bacterial Communities

Contact Info

Product

Resources

About