Dispersal of harmful fruit fly pests by international trade and a loop-mediated isothermal amplification assay to prevent their introduction

Blaser, Simon; Heusser, Cornelia; Diem, Hanspeter; Felten, Andreas von; Gueuning, Morgan; Andreou, Michael; Boonham, Neil; Tomlinson, J. A.; Müller, Pie; Utzinger, Jürg; Frey, Jürg E.; Frey, Beatrice; Bühlmann, Andreas

doi:10.4081/gh.2018.726

Cited by 9 publications

(9 citation statements)

References 19 publications

(46 reference statements)

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“…Enormous volumes of fruit are traded across borders daily (United Nations, ) and many of those consignments are infested with non‐native pests (Steffen et al ., ; Blaser et al ., ). Data from EUROPHYT, the European notification system for plant health interceptions, shows that fruit flies were among the most intercepted groups of harmful organisms on fruit and vegetables, ranging between 300 and 700 interceptions each year in the period 2013–2017 (EC‐DG SANTE, 2018a).…”

Section: Discussionmentioning

confidence: 97%

Survey on Bactrocera spp. (Tephritidae, Diptera) in Austria

et al. 2019

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Fruit fly species (Diptera: Tephritidae) of the genus Bactrocera are among the most serious orchard pests worldwide but are not native to Austria. The unexpected finding of one Bactrocera zonata adult in Vienna in 2011 initiated increasing survey efforts in Austria since 2012. Traps with male attracting lures were employed at several sites during the fruiting periods and catches were analysed. At sites in Vienna (urban area) Bactrocera specimens were trapped between 2012 and 2018, whereas no Bactrocera specimens were caught in agricultural areas or commercial orchards outside the city. Twelve specimens were identified as B. zonata and nine specimens as B. dorsalis sensu lato, though for the latter it was not possible to distinguish between B. dorsalis s.l. and B. carambolae using molecular analysis (ITS1). Molecular sequencing showed that the specimens caught were genetically different (with only a few exceptions), indicating genetically different origins. It is evident that the repeated findings of Bactrocera specimens in Vienna, where winter temperatures do not allow the establishment of tropical fruit flies, are linked to repeated entries of juveniles with infested fruits (in luggage or consignments). The results of our study reveal the need to strengthen phytosanitary import requirements for fruit commodities and travellers' luggage in order to protect fruit production in Austria and probably in other countries.

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Section: Discussionmentioning

confidence: 97%

Survey on Bactrocera spp. (Tephritidae, Diptera) in Austria

et al. 2019

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“…The most common application of DNA based detection in environmental biosecurity investigations is species identification (Linacre 2021). As a result, the use of LAMP to combat IWT extends to adulterated meat products (Cho et al 2014;Liu et al 2019;Nikunj and Vivek 2019;Sul et al 2019), detection of conservation significant species (But et al 2020;Wimbles et al 2021;Yu et al 2019), biosecurity screening (Blaser et al 2018b;Kyei-Poku et al 2020), invasive species detection in novel ecosystems (Rizzo et al 2021;Vythalingam et al 2021;Williams et al 2017), and disease monitoring (Sahoo et al 2016). Additionally, LAMP has a strong presence in bacterial and viral point-of-care detection methods research (Kashir and Yaqinuddin 2020;Nguyen et al 2019b).…”

Section: Lamp Research Applied To Environmental Biosecuritymentioning

confidence: 99%

“…Red fire ants are classed as a super pest with introductions as stowaways linked to early global trade routes (Gotzek et al 2015), continued interest in their further spread throughout Australia and Asia demands robust biosecurity testing (Wylie et al 2020). A large focus area is the detection of fruit fly species (Blaser et al 2018a;Blaser et al 2018b;Huang et al 2009;Sabahi et al 2018) (Blaser et al 2018a). Several studies have focused on the detection of fall armyworm (Agarwal et al 2022;Congdon et al 2021;Kim et al 2021).…”

Section: Border Surveillance Of Emerging Insect Incursionsmentioning

confidence: 99%

Shining a LAMP on the applications of isothermal amplification to monitoring illegal wildlife trade

Deliveyne¹,

Young²,

Austin³

et al. 2022

Preprint

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Environmental biosecurity risks associated with the Illegal Wildlife Trade (IWT) include the loss of biodiversity, threats to public health, and the proliferation of invasive alien species. To assist enforcement agencies in identifying trafficked species, rapid forensic techniques enable the detection of trace Environmental DNA (eDNA) where physical identification is not possible. Loop Mediated Isothermal Amplification (LAMP) is an emerging technique with recent applications in biosecurity and forensic sciences, and with potential to function as a field-based detection tool. Here we provide an overview of current research that applies LAMP to human and wildlife forensic science, including identification of ornamental wildlife parts, consumer products, and invasive species monitoring and biosecurity detection. We discuss the current scope of LAMP as applied to various wildlife crime scenarios and biosecurity checkpoint monitoring, highlight the specificity, sensitivity, and robustness for these applications, and review the potential utility of LAMP for rapid field-based detection within the IWT. Based on our assessment of the literature we recommend broader interest, research, and investment in LAMP as an appropriate field-based species detection method for a wide range of environmental biosecurity scenarios.

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“…Although it has many advantages, DNA barcoding is limited by the fact that the method can only identify species for which reference sequences as well as primer information are available (Armstrong & Ball, 2005; Blaser et al, 2018). Further limitations can arise when the degree of genetic differentiation of the barcoding fragment is too low to enable clear differentiation between closely related species (Armstrong & Ball, 2005).…”

Section: Introductionmentioning

confidence: 99%

First records of Ceroplastes ceriferus (Fabricius) (Hemiptera: Coccidae) and Ceroplastes japonicus (Gray) in Switzerland identified by DNA barcoding

et al. 2021

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Wax soft scales (Hemiptera: Coccidae) include many important pest species of agricultural and ornamental plants which spread along international trading networks of plants and plant material. Here we report the first findings of the wax scales Ceroplastes japonicus and Ceroplastes ceriferus for Switzerland. The two new findings of Ceroplastes were collected on Laurus nobilis and Vaccinium myrtillus, respectively, and identified by DNA barcoding.

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Dispersal of harmful fruit fly pests by international trade and a loop-mediated isothermal amplification assay to prevent their introduction

Cited by 9 publications

References 19 publications

Survey on Bactrocera spp. (Tephritidae, Diptera) in Austria

Survey on Bactrocera spp. (Tephritidae, Diptera) in Austria

Shining a LAMP on the applications of isothermal amplification to monitoring illegal wildlife trade

First records of Ceroplastes ceriferus (Fabricius) (Hemiptera: Coccidae) and Ceroplastes japonicus (Gray) in Switzerland identified by DNA barcoding

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