Dispersal and inhibitory roles of mannose, 2‐deoxy‐d‐glucose and N‐acetylgalactosaminidase on the biofilm of Desulfovibrio vulgaris

Abstract

Biofilms of sulfate-reducing bacteria (SRB) are often the major cause of microbiologically influenced corrosion. The representative SRB Desulfovibrio vulgaris has previously been shown to have a biofilm that consists primarily of protein. In this study, by utilizing lectin staining, we identified that the biofilm of D. vulgaris also consists of the matrix components mannose, fucose and N-acetylgalactosamine (GalNAc), with mannose predominating. Based on these results, we found that the addition of mannose and the nonmetabolizable mannose analog 2-deoxy-d-glucose inhibits the biofilm formation of D. vulgaris as well as that of D. desulfuricans; both compounds also dispersed the SRB biofilms. In addition, the enzyme N-acetylgalactosaminidase, which degrades GalNAc, was effective in dispersing D. vulgaris biofilms. Therefore, by determining composition of the SRB biofilm, effective biofilm control methods may be devised.

Glycoside hydrolase DisH from Desulfovibrio vulgaris degrades the N‐acetylgalactosamine component of diverse biofilms

“…Since the biofilm of D. vulgaris consists partially of GalNAc (Poosarla et al ., ), DisH 48–481 was investigated for its ability to disperse D. vulgaris biofilms. For 24 h biofilms formed at 30°C in modified Baar's medium, planktonic cells were removed and 50 ng μl −1 (Itoh et al ., ) of DisH 48–481 was added in non‐nutritive buffer to the biofilm and incubated at 30°C for 2 to 18 h. Also, protease (savinase) was used as a positive control and both inactivated DisH 48–481 and PBS buffer (pH 7.4) were used as negative controls.…”

“…Desulfovibrio vulgaris Hildenborough is a Gram‐negative model SRB whose biofilm consists primarily of proteins (Clark et al ., ), while the biofilm of another Desulfovibrio species, D. desulfuricans , consists of EPS that includes glucose (major carbohydrate), mannose and galactose (Beech et al ., ). Our work has determined that the biofilm of D. vulgaris Hildenborough also includes mannose, fucose and N ‐acetyl β‐ D ‐galactosamine (GalNac) (Poosarla et al ., ).…”

σ54‐Dependent regulator DVU2956 switches Desulfovibrio vulgaris from biofilm formation to planktonic growth and regulates hydrogen sulfide production

“…The SRB biofilm extracellular matrix consists of protein (Clark et al, 2007) and polymers of mannose, N-acetylβ-D-galactosamine (GalNAc) and fucose (Poosarla et al, 2017). Hence, SRB biofilms may be dispersed by protease (Clark et al, 2007) and by its own glycoside hydrolase (Zhu et al, 2018).…”

Rhamnolipids fromPseudomonas aeruginosaDisperse the Biofilms of Sulfate-Reducing Bacteria

“…Sulfate-reducing bacteria (SRB) are an important type of microorganisms causing iron corrosion on metal surfaces under both anaerobic and aerobic conditions [1][2][3] . Desulfovibrio vulgaris Hildenborough is a sequenced 4 Gram-negative SRB that has been used as an SRB model organism to study biocorrosion and bioremediation of toxic metal ions 4 as well as biofilm formation 5,6 and bioimmobilization in superfund sites 7 . It is also called the "petroleum pest" because it is commonly found in oil fields and causes 20 "souring" of petroleum and damage to topside equipment and pipelines 8 .…”

“…The biofilms of D. vulgaris consists primarily of protein 5 , mannose 6 , fucose 6 , and N-acetylgalactosamine 6 .…”