Disparity in the nasopharyngeal microbiota between healthy cattle on feed, at entry processing and with respiratory disease

Zeineldin, Mohamed; Lowe, James; Godoy, M de; Maradiaga, Nidia; Ramirez, Chelsey; Ghanem, Mohamed M.; El-Raof, Yassein Abd; Aldridge, Brian

doi:10.1016/j.vetmic.2017.07.006

Cited by 62 publications

(77 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The high abundance of Moraxella in the nasal passageways observed in the current study agreed with findings from previous research. Moraxella is often found to be one of the most abundant genera in the URT of cattle [14,[40][41][42]. The role of this genus in BP has been brought into question before, with one previous study finding an association between Moraxella and the development of pneumonia and/or otitis in early life of dairy calves [43].…”

Section: Discussionmentioning

confidence: 99%

Topography of the respiratory tract bacterial microbiota in cattle

McMullen

Alexander

Léguillette

et al. 2020

Preprint

View full text Add to dashboard Cite

BackgroundBacterial bronchopneumonia (BP) is the leading cause of morbidity and mortality in cattle. While the bacterial composition of the bovine upper respiratory tract (URT) has not been studied in detail, the nasopharynx is generally accepted as the primary source of pathogenic bacteria that cause BP.However, it has recently been shown in humans that the oropharynx may act as the primary reservoir for pathogens that reach the lung. The objective was therefore to describe the bacterial microbiota present along the entire cattle respiratory tract to determine which URT niches may contribute the most to the composition of the lung microbiota. MethodsSeventeen upper and lower respiratory tract locations were sampled from 15 healthy feedlot steer calves. Samples were collected using a combination of swabs, protected specimen brushes, and saline washes. DNA was extracted from each sample and the 16S rRNA gene (V3-V4) was sequenced.Community composition, alpha-diversity, and beta-diversity were compared among sampling locations. ResultsMicrobiota composition differed across sampling locations, with physiologically and anatomically distinct locations showing different relative abundances of 1,137 observed sequence variants (SVs).An analysis of similarities showed that the lung was more similar to the nasopharynx (R-statistic = 0.091) than it was to the oropharynx (R-statistic = 0.709) or any other URT sampling location. Five distinct metacommunities were identified across all samples after clustering at the genus level using Dirichlet multinomial mixtures. This included a metacommunity found primarily in the lung and nasopharynx that was dominated by Mycoplasma . Further clustering at the SV level showed a shared metacommunity between the lung and nasopharynx that was dominated by Mycoplasma dispar .Other metacommunities found in the nostrils, tonsils, and oral microbiotas were dominated by Moraxella , Fusobacterium , and Streptococcus , respectively.

show abstract

Section: Discussionmentioning

confidence: 99%

Topography of the respiratory tract bacterial microbiota in cattle

McMullen

Alexander

Léguillette

et al. 2020

Preprint

View full text Add to dashboard Cite

show abstract

“…The dynamics of change in the NP microbiota between clinically healthy calves and those that develop BRD were reported in detail in our previous published study (12). In this study, we compared the relative abundance of the most abundant bacterial phyla that accounted for more than 1% of the total (Firmicutes, Actinobacteria, Tenericutes, Proteobacteria, and Bacteroidetes) across all three groups.…”

Section: Comparison Of Np Microbiota Across the Different Groupsmentioning

confidence: 89%

“…This study was a part of a larger experiment that examined the clinical and microbial predictors of susceptibility to BRD in beef cattle (12,13). The use of the animals, and all experiments procedures were performed in accordance with relevant guidelines, and under the approval of, the University of Illinois Institutional Animal Care and Use Committee (IACUC Protocol: #15064).…”

Section: Study Populations and Sample Collectionmentioning

confidence: 99%

“…All calves were processed within 24 h after arrival to the farm. During the first month after arrival, all calves were monitored daily for signs of respiratory disease according to industry-standard protocols (anorexia, nasal discharge, change in respiratory pattern, rectal temperature ≥ 40 • C and Whisper lung score ≥ 3) (12). Deep NP samples were collected with double-guarded sterile culture swab (Kalayjian Industries, Inc. U.S.A.) from calves diagnosed with BRD at the initial diagnosis and prior to treatment (BRD group, n = 9) according to published techniques (14).…”

Section: Study Populations and Sample Collectionmentioning

confidence: 99%

“…The nasopharyngeal microbiota is believed to be a key player in the health of the upper respiratory tract, and has been shown to be significantly modified during episodes of immunological stress and clinical respiratory disease (21). As a result of these observations, it has been suggested that disturbances in NP microbial communities may contribute to the pathophysiology of BRD in feedlot cattle (12). Although several studies have investigated the bovine NP microbiota in the predisease and disease states (22)(23)(24), little information is available on the change of NP microbiota associated with clinical recovery from BRD.…”

Section: Overall Taxonomic Classification and Diversity Of Np Microbiotamentioning

confidence: 99%

See 2 more Smart Citations

Effects of Tilmicosin Treatment on the Nasopharyngeal Microbiota of Feedlot Cattle With Respiratory Disease During the First Week of Clinical Recovery

2020

Self Cite

View full text Add to dashboard Cite

While the nasopharyngeal (NP) microbiota is believed to be a key player in bovine respiratory health, there is limited published information about the change of NP microbiota associated with clinical recovery from bovine respiratory disease (BRD). The objective of this study was to evaluate the effect of tilmicosin treatment on the NP microbiota composition and diversity of BRD-affected calves during the first week of clinical recovery. Deep NP swabs were collected from diseased calves at the initial diagnosis of BRD, and again 7 days after the administration of a single dose of tilmicosin. As an experimental control, samples were collected from clinically healthy, pen-matched calves at the time of initial BRD diagnosis. In general, the NP microbiota from the control calves were more diverse than the NP microbiota from tilmicosin treated and BRD-affected calves. Principle coordinate analysis (PCOA) of Bray-Curtis and Jaccard dissimilarity also revealed that the overall composition of NP microbial communities in tilmicosin-treated calves closely resembled that of BRD-affected calves but differed significantly from pen-matched healthy calves. Overall, it appeared that there were only minor changes in NP microbial communities following tilmicosin treatment and, during the early phase of clinical recovery the NP microbiota in treated animals was disparate from that observed in healthy control calves. Understanding the potential impact of this prolonged recovery in mucosal microbiota would be important in optimizing the use of antimicrobials in health management programs in the feedlot industry.

show abstract