Disorders on cardiovascular parameters in rats and in human blood cells caused by Lachesis acrochorda snake venom

Angel-Camilo, Karen Leonor; Guerrero-Vargas, Jimmy Alexander; Carvalho, Emanuella Feitosa de; Lima-Silva, Karine; Siqueira, Rodrigo José Bezerra de; Freitas, Lyara Barbosa Nogueira; Sousa, João Antônio Costa de; Mota, Mário Rogério Lima; Santos, Armênio Aguiar dos; Neves-Ferreira, Ana Gisele C.; Havt, Alexandre; Leal, Luzia Kalyne Almeida Moreira; Magalhães, Pedro Jorge Caldas

doi:10.1016/j.toxicon.2020.06.009

Cited by 8 publications

(1 citation statement)

References 38 publications

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“…Two bands were cut in the region bounded by the 45 and the 66 kDa molecular mass standards in each gel lane. Each gel band was then individually submitted to in-gel digestion with trypsin, as previously described [ 38 ]. After gel extraction, the peptides were dried in a vacuum centrifuge concentrator and suspended in 12 µL of 1% formic acid.…”

Section: Methodsmentioning

confidence: 99%

Combination of E- and NS1-Derived DNA Vaccines: The Immune Response and Protection Elicited in Mice against DENV2

Pinto

Azamor

Lima

et al. 2022

Viruses

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The occurrence of dengue disease has increased radically in recent decades. Previously, we constructed the pE1D2 and pcTPANS1 DNA vaccines encoding the DENV2 envelope (E) and non-structural 1 (NS1) proteins, respectively. To decrease the number of plasmids in a tetravalent candidate vaccine, we constructed a bicistronic plasmid, pNS1/E/D2, encoding these two proteins simultaneously. We evaluated the protective immunity induced in mice vaccinated with the pNS1/E/D2 candidate and compared to the responses elicited by immunization with the former vaccines isolated or in combination. We transfected BHK-21 cells with the different plasmids and detected recombinant proteins by immunofluorescence and mass spectrometry assays to confirm antigen expression. BALB/c mice were inoculated with the DNA vaccines followed by a lethal DENV2 challenge. ELISA, PRNT50, and IFN-gamma ELISPOT assays were performed for the investigation of the humoral and cellular responses. We observed the concomitant expression of NS1 and E proteins in pNS1/E/D2-transfected cells. All E-based vaccines induced anti-E and neutralizing antibodies. However, anti-NS1 antibodies were only observed after immunization with the pcTPANS1 administered alone or combined with pE1D2. In contrast, splenocytes from pNS1/E/D2- or pcTPANS1 + pE1D2-vaccinated animals responded to NS1- and E-derived synthetic peptides. All the DNA vaccines conferred protection against DENV2.

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Section: Methodsmentioning

confidence: 99%