Dismutation Properties of Purified and GDA Modified CuZnSOD from Chicken Heart

Demirel, L. Arzu Bozkaya; Tarhan, Leman

doi:10.1081/bio-200039654

Cited by 8 publications

(1 citation statement)

References 31 publications

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“…The SOD enzyme was not inhibited by DTT and betamercaptoethanol, but inhibited by CN(-) and H 2 O 2 [47]. SOD purified from chicken heart has a molecular weight 31.0 +/-1.0 kDa and is composed of two equally sized subunits each having 1.1 +/-0.03 and 0.97 +/-0.02 atoms of Cu and Zn elements, respectively [48]. The Mn-SOD cDNA in chicken heart was Vol.…”

Section: Sod In Avian Biologymentioning

confidence: 99%

Antioxidant Systems in Poultry Biology: Superoxide Dismutase

Surai¹

2016

J Anim Res Nutr

165

135

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Commercial poultry production is associated with various stresses responsible for decreasing productive and reproductive performance of growing chicks, breeders and commercial layers. A growing body of evidence shows that most of stresses in poultry production at the cellular level are associated with oxidative stress. Recently, a concept of the cellular antioxidant defense has been revised with special attention paid to cellular redox status maintenance and cell signaling. In fact, antioxidant systems of the living cell are based on three major levels of defense and superoxide dismutase (SOD) is shown to belong to the first level of the antioxidant defense network. Cellular antioxidant defenses are shown to include several options and vitagene activation in stress conditions is considered as a fundamental adaptive mechanism. The vitagene family includes various genes regulating synthesis of protective molecules including thioredoxins, sirtuins, heat shock proteins and SOD. However, by the time of writing no comprehensive review on the roles and effects of SOD in poultry biology has appeared. Therefore, the aim of this review is a critical analysis of the role of SOD in poultry biology with specific emphasis to its functions as an essential part of the vitagene network. From the analysis of the recent data related to SOD in poultry physiology and adaptation to stresses it is possible to conclude that: a) SOD as important vitagene is the main driving force in cell/body adaptation to various stress conditions. Indeed, in stress conditions additional synthesis of SOD is an adaptive mechanism to decrease ROS formation; b) If the stress is too high SOD activity is decreased and apoptosis is activated; c) there are tissue-specific differences in SOD expression which also depends on the strength of such stress-factors as heat, heavy metals, mycotoxins and other stressors; d) SOD is shown to provide an effective protection against lipid peroxidation in chicken embryonic tissues and semen; e) SOD is shown to be protective in heat and cold stress, toxicity stress as well as in other oxidative-stress related conditions in poultry production; f) there are complex interactions inside the antioxidant network of the cell/body to ensure an effective maintenance of homeostasis in stress conditions. Indeed, in many cases, nutritional antioxidants (vitamin E, selenium, carotenoids, phytochemicals, etc.) in the feed can increase SOD expression; g) nutritional means of SOD upregulation in stress conditions of poultry production and physiological and commercial consequences await further investigation; h) vitagene upregulation in stress conditions is emerging as an effective means for stress management.

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Section: Sod In Avian Biologymentioning

confidence: 99%

Antioxidant Systems in Poultry Biology: Superoxide Dismutase

Surai¹

2016

J Anim Res Nutr

165

135

View full text Add to dashboard Cite

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Purification and biochemical characterization of a novel copper, zinc superoxide dismutase from liver of camel (Camelus dromedarius): An antioxidant enzyme with unique properties

Chafik

Essamadi

Çelik

et al. 2019

Bioorganic Chemistry

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Synthesis and tyrosinase inhibitory properties of novel isoquinoline urea/thiourea derivatives

Genc

Zengin

Yavuz

et al. 2013

Artificial Cells, Nanomedicine, and Biotechnology

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A new series of isoquinoline urea/thiourea derivatives (1-11) were synthesized, and their inhibitory effects on tyrosinase were evaluated. Isoquinoline urea/thiourea derivatives were obtained as a result of the reaction of 5-aminoisoquinoline with isocyanates or isothiocyanates. The result showed that all the synthesized compounds inhibited the tyrosinase enzyme activity. Among the compounds synthesized, 1-(4-chlorophenyl)-3-(isoquinolin-5-yl)thiourea (3) was found to be the most active one (Ki = 119.22 μM), and the inhibition kinetics analyzed using Lineweaver-Burk double reciprocal plots revealed that compound 3 was a competitive inhibitor. We also calculated HOMO-LUMO energy levels, some selected the synthesized compounds (1, 4, 11, 3, 6, 2) using Gaussian software.

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Dismutation Properties of Purified and GDA Modified CuZnSOD from Chicken Heart

Cited by 8 publications

References 31 publications

Antioxidant Systems in Poultry Biology: Superoxide Dismutase

Antioxidant Systems in Poultry Biology: Superoxide Dismutase

Purification and biochemical characterization of a novel copper, zinc superoxide dismutase from liver of camel (Camelus dromedarius): An antioxidant enzyme with unique properties

Synthesis and tyrosinase inhibitory properties of novel isoquinoline urea/thiourea derivatives

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