Research on human resource practices within family businesses is limited, particularly in the wine industry. In this comparative study, 57 US and 37 Australian small and mid-sized family wineries responded to a comprehensive survey of their human resource systems. In addition to contrasting HR practices between family wineries in different countries, this study analyzed the impact of these activities on company sales and profitability. Results were consistent with the hypotheses: US firms were more likely to formalize HR practices than their Australian counterparts, Australian wineries emphasize worker training whereas US wineries focus on hiring qualified employees and both groups connected formalized HR practices to effectiveness and profitability. Finally, the US wineries reported higher profitability than Australian wineries. Implications of the results are discussed and directions for future research are suggested.Keywords Human resource practices . US family wineries . Australian family wineries As organizations grow, they tend to adopt formal policies and practices to manage growth more effectively (Frankl et al. 2000; Kuratko 1990, 2003). One of these areas is the formalization and implementation of human resource practices, which revolve around staffing, training, compensation, benefits, and employee relations. Research has shown that adoption of formalized and consistent procedures for hiring, developing, appraising and compensating employees can result in higher productivity and increased financial returns to the firm (