Disintegration of Tissue Using High Intensity Focused Ultrasound: Two Approaches That Utilize Shock Waves

Maxwell, Adam D.; Sapozhnikov, Oleg A.; Bailey, Michael R.; Crum, Lawrence A.; Xu, Zhen; Fowlkes, J. Brian; Cain, Charles A.; Khokhlova, Vera A.

doi:10.1121/1.4788649

Cited by 91 publications

(79 citation statements)

References 36 publications

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“…The treatment was shown to be well tolerated; however, to achieve the focal peak negative pressures sufficient for cavitation cloud formation, very large and highly focused (f-number 0.8) transducers are required, as well as the amplifiers capable of providing extremely high instantaneous power (on the order of several kilowatts). Boiling histotripsy requires significantly less power (on the order of hundreds of watts), making it possible to implement it clinically by using commercially available HIFU systems (19,20). Moreover, the requirements on the size, focusing gain (f-number 1), and frequency of the HIFU source are less restrictive, which is an advantage when only a limited acoustic window is available.…”

mentioning

confidence: 99%

Ultrasound-guided tissue fractionation by high intensity focused ultrasound in an in vivo porcine liver model

Khokhlova

Wang

Simon

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

102

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Significance High intensity focused ultrasound (HIFU) therapy is a promising, clinically adopted method of noninvasive tissue ablation used to treat both benign and malignant conditions. This work presents, to our knowledge, the first in vivo validation of a previously developed HIFU-based method that allows for noninvasive fractionation of targeted tissue into subcellular debris—boiling histotripsy—in a large animal model. While fractionating the targeted soft tissue, boiling histotripsy is shown to spare the adjacent connective tissue structures such as blood vessels. The process can be readily targeted and monitored by B-mode ultrasound. The resulting tissue debris are liquid, which provides a potential clinical benefit over thermal ablation in the treatment of tumors that exert uncomfortable pressure on surrounding tissues.

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mentioning

confidence: 99%

Ultrasound-guided tissue fractionation by high intensity focused ultrasound in an in vivo porcine liver model

Khokhlova

Wang

Simon

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

102

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“…These effects have been broadly utilized for non-contact therapeutic applications such as shockwave lithotripsy [3,4], hyperthermia-based tumor treatment [5][6][7], and thrombolysis [8,9]. In the local disruption process, cavitational disturbances are of especial interests because they can disintegrate tissues non-thermally (known as histotripsy) [9][10][11][12] and facilitate thermal ablation processes collaboratively [12]. Furthermore, the cavitational impacts, together with shock-induced effects, have offered great potentials for in vitro cellular engineering in terms of selective cell detachment, patterning, and harvesting for cell-based assays and secondary analyses [13][14][15][16].…”

Section: Introductionmentioning

confidence: 99%

Micro-ultrasonic cleaving of cell clusters by laser-generated focused ultrasound and its mechanisms

Baac

Lee

Guo

2013

Biomed. Opt. Express

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Laser-generated focused ultrasound (LGFU) is a unique modality that can produce single-pulsed cavitation and strong local disturbances on a tight focal spot (<100 μm). We utilize LGFU as a non-contact, non-thermal, high-precision tool to fractionate and cleave cell clusters cultured on glass substrates. Fractionation processes are investigated in detail, which confirms distinct cell behaviors in the focal center and the periphery of LGFU spot. For better understanding of local disturbances under LGFU, we use a high-speed laser-flash shadowgraphy technique and then fully visualize instantaneous microscopic processes from the ultrasound wave focusing to the micro-bubble collapse. Based on these visual evidences, we discuss possible mechanisms responsible for the focal and peripheral disruptions, such as a liquid jet-induced wall shear stress and shock emissions due to bubble collapse. The ultrasonic micro-fractionation is readily available for in vitro cell patterning and harvesting. Moreover, it is significant as a preliminary step towards high-precision surgery applications in future. time-resolved imaging and analysis of hydrodynamic effects," Biophys.

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“…Shock-scattering pulses up to 20 ls in duration have been utilized. 31 The longest pulses considered here were 5 ls in duration due to the large computational time ($20 h/calculation). Additional effects, such as the transfer of vapor, were not considered in these calculations, 32 and the assumptions of air as the gas content may not be appropriate in some physiologic conditions, particularly in ischemic environments.…”

Section: Discussionmentioning

confidence: 99%

The influence of gas diffusion on bubble persistence in shock-scattering histotripsy

Bader

Bollen

2018

The Journal of the Acoustical Society of America

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Bubble cloud persistence reduces the efficacy of mechanical liquefaction with shock-scattering histotripsy. In this study, the contribution of gas transfer to bubble longevity was investigated in silico by solving the equations for bubble oscillations and diffusion in parallel. The bubble gas content increased more than 5 orders of magnitude during the expansion phase, arresting the inertial collapse. The residual gas bubble required more than 15 ms for passive dissolution post excitation, consistent with experimental observation. These results demonstrate gas diffusion is an important factor in the persistence of histotripsy-induced cavitation.

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Disintegration of Tissue Using High Intensity Focused Ultrasound: Two Approaches That Utilize Shock Waves

Cited by 91 publications

References 36 publications

Ultrasound-guided tissue fractionation by high intensity focused ultrasound in an in vivo porcine liver model

Ultrasound-guided tissue fractionation by high intensity focused ultrasound in an in vivo porcine liver model

Micro-ultrasonic cleaving of cell clusters by laser-generated focused ultrasound and its mechanisms

The influence of gas diffusion on bubble persistence in shock-scattering histotripsy

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