Disintegration of Biomacromolecules by Dielectric Barrier Discharge Plasma in Helium at Atmospheric Pressure

Hou, Ying; Xiao, Dong; Hong, Yi; Li, Shuang; Ren, Chunsheng; Zhang, Dai Jia; Xiu, Zhi Long

doi:10.1109/tps.2008.927630

Cited by 20 publications

(10 citation statements)

References 12 publications

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“…Only about 1% of the cells remained alive at 210 s. The survival rate thus showed a saddle-shaped curve that was also observed in our earlier studies (Dong et al 2009Hou et al 2008).…”

Section: Resultssupporting

confidence: 87%

Dielectric barrier discharge plasma as a novel approach for improving 1,3-propanediol production in Klebsiella pneumoniae

Dong

Xiu

et al. 2010

Biotechnol Lett

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Dielectric barrier discharge plasma was used to generate a stable strain of Klebsiella pneumoniae (designated to as Kp-M2) with improved 1,3-propanediol production. The specific activities of glycerol dehydrogenase, glycerol dehydratase and 1,3-propanediol oxidoreductase in the crude cell extract increased from 0.11, 9.2 and 0.15 U mg(-1), respectively, for wild type to 0.67, 14.4 and 1.6 U mg(-1) for Kp-M2. The glycerol flux of Kp-M2 was redistributed with the flux to the reductive pathway being increased by 20% in batch fermentation. The final 1,3-propanediol concentrations achieved by Kp-M2 in batch and fed-batch fermentations were 19.9 and 76.7 g l(-1), respectively, which were higher than those of wild type (16.2 and 49.2 g l(-1)). The results suggested that dielectric barrier discharge plasma could be used as an effective approach to improve 1,3-propanediol production in K. pneumoniae.

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“…Only about 1% of the cells remained alive at 210 s. The survival rate thus showed a saddle-shaped curve that was also observed in our earlier studies (Dong et al 2009Hou et al 2008).…”

Section: Resultssupporting

confidence: 87%

Dielectric barrier discharge plasma as a novel approach for improving 1,3-propanediol production in Klebsiella pneumoniae

Dong

Xiu

et al. 2010

Biotechnol Lett

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“…Generally, DBD treatment can induce both inactivation and repair mechanisms in biological systems. For example, Hou et al observed the first decrease and subsequent increase in survival rate for helium-DBD treatment of K. pneumoniae 31 . So it is reasonable to assume that the residual effect may also include both residual inactivation effect and residual repair effect.…”

Section: Discussionmentioning

confidence: 99%

New insight into the residual inactivation of Microcystis aeruginosa by dielectric barrier discharge

Hong

Huang

2015

Sci Rep

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We report the new insight into the dielectric barrier discharge (DBD) induced inactivation of Microcystis aeruginosa, the dominant algae which caused harmful cyanobacterial blooms in many developing countries. In contrast with the previous work, we employed flow cytometry to examine the algal cells, so that we could assess the dead and living cells with more accuracy, and distinguish an intermediate state of algal cells which were verified as apoptotic. Our results showed that the numbers of both dead and apoptotic cells increased with DBD treatment delay time, and hydrogen peroxide produced by DBD was the main reason for the time-delayed inactivation effect. However, apart from the influence of hydrogen peroxide, the DBD-induced initial injures on the algal cells during the discharge period also played a considerable role in the inactivation of the DBD treated cells, as indicated by the measurement of intracellular reactive oxygen species (ROS) inside the algal cells. We therefore propose an effective approach to utilization of non-thermal plasma technique that makes good use of the residual inactivation effect to optimize the experimental conditions in terms of discharge time and delay time, so that more efficient treatment of cyanobacterial blooms can be achieved.Water eutrophication has become a serious global problem, which can cause harmful cyanobacterial blooms 1,2 . Among the blooming cyanobacteria, Microcystis aeruginosa produces and releases toxins which pose constant threat to our environment and human health. Therefore, there is an urgent need to develop efficient techniques to control and reduce the adverse impact of blooms. To suppress or remove cyanobacteria blooms, various methods have been adopted such as chemical treatment [3][4][5] , UV radiation 6,7 , ultrasound irradiation 8-10 , electron beam irradiation 11 and non-thermal plasma oxidation technology 2,12-16 . However, the chemical methods such as excessive use of algaecides can lead to secondary pollution, while the physical methods such as UV radiation, sonication and electron-beam irradiation have the limitation for bloom control with high efficiency or on a large scale.As an emerging technology, plasma oxidation technology has received special attention and been applied extensively in wastewater treatment 17 . Being one of advanced oxidation processes (AOPs), it possesses both the physical and chemical processing traits, and exhibits the advantages in simpler equipment, easier operation, higher energy efficiency and environmental compatibility 2,18,19 . Among various plasma techniques, the non-thermal atmospheric dielectric barrier discharge (DBD) may be a most promising one. In fact, non-thermal DBD has been widely applied in inactivation of pathogen 20,21 , chemical synthesis 22 , film deposition 23 , material surface modification 24 and etc. When plasma takes place either over the solution surface or in the solution, a variety of physical and/or chemical processes are initiated. Besides

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“…Proteins of large molecular weight (>44 kDa) are easily degraded, while proteins of small molecular weight (<20 kDa) are not significantly degraded. (30,76) Effects on secondary structure. The effects of NTAP on protein secondary structure have been confirmed by considerable studies, which is mainly exhibited as various alterations or destructions of protein folding.…”

Section: Gas Phasementioning

confidence: 99%

Effects of non-thermal atmospheric plasma on protein

Dai

et al. 2022

J. Clin. Biochem. Nutr.

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Currently, the advancement in non-thermal atmospheric plasma technology enables plasma treatments on some heat-sensitive targets, including biological substances, without unspecific damage caused by thermal effect. The significant effects of nonthermal atmospheric plasma modulating biological events have been demonstrated by considerable studies. Protein, one of the most important biomolecules, participates in the majority of the life-sustaining activities in all organisms, whose functions are derived from the diverse biochemical properties of amino acid compositions and four-tiered protein structure hierarchy. Therefore, the knowledge of how non-thermal atmospheric plasma affects protein greatly benefits the understanding and application of the non-thermal atmospheric plasma's effect in biological area. In this review, we summarize recent research progress on the effects of non-thermal atmospheric plasma, particularly its reactive species, on biochemical and biophysical characteristics of proteins at different structural levels that leads to their functional changes. Moreover, the physiological effects of non-thermal atmospheric plasma at cellular or organism level driven by the manipulations on protein and their relative application prospects are reviewed. Despite the exceptional application potential, the exploration of the non-thermal atmospheric plasma's effect on protein still confronts with difficulties due to the limited knowledge of the underlying mechanisms and the complexity of non-thermal atmospheric plasma operation systems, which requires further studies and standardization of non-thermal atmospheric plasma treatments.

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Disintegration of Biomacromolecules by Dielectric Barrier Discharge Plasma in Helium at Atmospheric Pressure

Cited by 20 publications

References 12 publications

Dielectric barrier discharge plasma as a novel approach for improving 1,3-propanediol production in Klebsiella pneumoniae

Dielectric barrier discharge plasma as a novel approach for improving 1,3-propanediol production in Klebsiella pneumoniae

New insight into the residual inactivation of Microcystis aeruginosa by dielectric barrier discharge

Effects of non-thermal atmospheric plasma on protein

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