Background: Hospital indoor air consists of a diverse and wide range of micro-organism. Since time the concept of sterilization of rooms was introduced by Lister, it has been a topic of worry regarding the sterilization of operation theatres. In view of this, we carried a microbiological surveillance of the overall efficiency of various disinfection methods of instrument sterilization in a health care centre where ophthalmic surgeries were carried out.
Materials & Methods:The present study was carried out among patients that underwent various major and minor ophthalmic surgical procedures. In all the three operating room, sampling was conducted 184 times in each room. Before the starting of the next surgical procedure, sealing of the room was done before 12 -14 hours of the surgery. Weekly assessment of Environmental Bacteria Carrying Particle (BCP) load was done by sedimentation method prior to the commencement of the surgeries. In the surgical operating room, swabs were collected from the surfaces of the representative areas. Once in every two months, the evaluation of the contamination was done. Results: In all the three operating room, sampling was conducted 184 times in each room. 8 sampling procedures in room 3 exhibited the highest bacterial count among the three rooms followed by 4 and 6 in room 1 and room 2 respectively. S. aureus was detected in 4 and 6 procedural cases in room 2