Disinfection Processes

Munakata, Naoko; Kuo, Jeff

doi:10.2175/106143014x14031280667651

Cited by 1 publication

(1 citation statement)

References 92 publications

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“…Hospital acquired infections such as methicillin‐resistant Staphylococcus aureus (MRSA) are a great challenge for public health , and the search for new methods to control infections caused by pathogenic microorganisms is a priority . A very promising approach is the photodynamic inactivation of microorganisms (PDI), which is receiving considerable attention since it is unlikely that resistance can be developed to this treatment .…”

Section: Introductionmentioning

confidence: 99%

Real‐time imaging of photodynamic action in bacteria

Gollmer

Felgentraeger

Maisch

et al. 2016

Journal of Biophotonics

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Fluorescence imaging studies of the processes leading to photodynamic inactivation of bacteria have been limited due to the small size of microorganisms as well as by the faint fluorescence of most photosensitizers. A versatile method based on highly-sensitive fluorescence microscopy is presented which allows to study, in real time, the incorporation of photosensitizers inside S. aureus upon photodynamic action. The method takes advantage of the fluorescence enhancement of phenothiazine and porphyrin photosensitizers upon entering the bacterial cytosol after the cell wall has been compromised. In combination with typical assays, such as the addition of specific enhancers of reactive oxygen species, it is possible to extract mechanistic information about the pathway of photodynamic damage at the single-cell level. Imaging experiments in deuterated buffer strongly support a Type-I mechanism for methylene blue and a very minor role of singlet oxygen.

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Section: Introductionmentioning

confidence: 99%